Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACEO96_RS12725 Genome accession   NZ_CP168657
Coordinates   2778057..2779067 (-) Length   336 a.a.
NCBI ID   WP_373433102.1    Uniprot ID   -
Organism   Vibrio anguillarum strain VA1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2773057..2784067
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEO96_RS12700 (ACEO96_12700) aroK 2773924..2774445 (-) 522 WP_010317691.1 shikimate kinase AroK -
  ACEO96_RS12705 (ACEO96_12705) pilQ 2774632..2776347 (-) 1716 WP_013855889.1 type IV pilus secretin PilQ Machinery gene
  ACEO96_RS12710 (ACEO96_12710) pilP 2776371..2776892 (-) 522 WP_373433101.1 pilus assembly protein PilP Machinery gene
  ACEO96_RS12715 (ACEO96_12715) pilO 2776879..2777472 (-) 594 WP_013855887.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACEO96_RS12720 (ACEO96_12720) pilN 2777465..2778067 (-) 603 WP_047690569.1 PilN domain-containing protein Machinery gene
  ACEO96_RS12725 (ACEO96_12725) pilM 2778057..2779067 (-) 1011 WP_373433102.1 type IV pilus assembly protein PilM Machinery gene
  ACEO96_RS12730 (ACEO96_12730) - 2779217..2781715 (+) 2499 WP_373433103.1 penicillin-binding protein 1A -
  ACEO96_RS12735 (ACEO96_12735) - 2781753..2782214 (-) 462 Protein_2429 transposase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37433.02 Da        Isoelectric Point: 7.9013

>NTDB_id=1044837 ACEO96_RS12725 WP_373433102.1 2778057..2779067(-) (pilM) [Vibrio anguillarum strain VA1]
MGKSMVTGIDIGHHSIKAVVLKLAGDSYALVGYKELFITEDIFSDNHTLDYQKIVKKLKELRKGLPLFSRKVAISVPDNA
VISKVLQIDSDLQAREKEFAIYQAFSHQSPFPIEELSLDFVKVMEKSVSRSSTVSYQVYATRKDVVESRALAAQKAGFLP
IAVDVQIHSLLNIWQLASRAQHRSDWMLMDVGHTQTALCIDFTDKAPFHKEIPLGCQWLSQDSAMSGTNAELADKNERFV
HELIERLQRQLQLFTSVNGAGSLAGIWLSGGGADTPMLAQEISRRLKLECEILDPFLMFNNKLAKRQRGKQSGQSFSTAA
GLALRGIEWLESSHVA

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=1044837 ACEO96_RS12725 WP_373433102.1 2778057..2779067(-) (pilM) [Vibrio anguillarum strain VA1]
ATGGGTAAATCAATGGTTACGGGTATAGATATTGGCCACCATAGTATTAAAGCGGTGGTGTTAAAGCTAGCGGGTGATTC
ATATGCACTCGTCGGTTATAAAGAATTATTTATTACTGAGGACATATTCTCCGATAACCACACGCTGGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAGAAAGGGTTTACCACTATTTAGTCGCAAAGTCGCCATATCGGTACCGGACAATGCG
GTAATAAGTAAGGTATTACAGATAGATAGTGACCTGCAGGCGCGTGAAAAAGAGTTTGCTATTTATCAGGCATTTTCTCA
TCAGTCACCGTTCCCGATAGAAGAACTGAGTCTCGATTTCGTCAAAGTGATGGAAAAAAGCGTAAGTCGCAGTAGCACCG
TAAGTTATCAAGTTTACGCCACTCGTAAAGACGTCGTGGAAAGCCGTGCGTTAGCGGCTCAAAAAGCGGGCTTTTTACCG
ATCGCGGTTGATGTGCAAATACACAGTTTGTTAAACATTTGGCAGTTGGCGAGCAGGGCACAACATCGCAGCGATTGGAT
GCTCATGGATGTGGGCCATACCCAAACGGCGCTCTGTATTGATTTTACTGATAAAGCCCCTTTTCATAAAGAGATCCCTC
TCGGTTGTCAGTGGTTGTCCCAAGACAGCGCGATGTCGGGCACCAATGCGGAATTAGCCGATAAAAACGAGCGCTTTGTG
CATGAGTTGATCGAGCGACTTCAACGTCAGTTGCAGCTTTTCACCTCCGTCAATGGCGCGGGCAGTTTAGCAGGCATTTG
GCTCTCAGGTGGTGGTGCAGATACTCCGATGTTGGCGCAAGAGATCTCGCGGCGTCTCAAGCTTGAGTGCGAGATTCTCG
ATCCCTTTCTTATGTTTAACAATAAGTTAGCGAAACGCCAGCGAGGTAAGCAGTCTGGGCAGAGTTTTAGCACCGCAGCT
GGTCTTGCACTGCGTGGTATCGAGTGGTTGGAGTCTTCTCATGTTGCATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

66.864

100

0.673
  pilM Vibrio campbellii strain DS40M4

52.381

100

0.524

Multiple sequence alignment