Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   R8493_RS05725 Genome accession   NZ_AP028341
Coordinates   1100941..1101891 (+) Length   316 a.a.
NCBI ID   WP_317647068.1    Uniprot ID   -
Organism   Campylobacter coli strain BCH-10328     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1086714..1112022 1100941..1101891 within 0


Gene organization within MGE regions


Location: 1086714..1112022
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8493_RS05655 (B10328_10980) - 1086714..1087643 (-) 930 WP_317647056.1 glycosyltransferase family 2 protein -
  R8493_RS05660 (B10328_10990) pglH 1087636..1088712 (-) 1077 WP_317647057.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  R8493_RS05665 (B10328_11000) pglK 1088709..1090409 (-) 1701 WP_317647058.1 ABC-type lipopolysaccharide transporter PglK -
  R8493_RS05670 (B10328_11010) galE 1090403..1091389 (-) 987 WP_025399072.1 UDP-glucose 4-epimerase GalE -
  R8493_RS05675 (B10328_11020) - 1091448..1092239 (-) 792 WP_025399073.1 3'-5' exonuclease -
  R8493_RS05680 (B10328_11030) waaC 1092304..1093332 (+) 1029 WP_317647061.1 lipopolysaccharide heptosyltransferase I -
  R8493_RS05685 (B10328_11040) htrB 1093322..1094209 (+) 888 WP_317647062.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  R8493_RS05690 (B10328_11050) - 1094206..1094767 (+) 562 Protein_1098 glycosyltransferase family 2 protein -
  R8493_RS09120 - 1094875..1094973 (+) 99 Protein_1099 glycosyltransferase family 2 protein -
  R8493_RS05695 (B10328_11060) - 1094966..1096027 (+) 1062 WP_220575214.1 glycosyltransferase family 4 protein -
  R8493_RS05700 (B10328_11070) - 1095993..1096886 (-) 894 WP_072230871.1 glycosyltransferase family 2 protein -
  R8493_RS05705 (B10328_11080) - 1096879..1098051 (-) 1173 WP_371929706.1 glycosyltransferase -
  R8493_RS05710 (B10328_11090) - 1098165..1099136 (-) 972 WP_317647065.1 glycosyltransferase family 2 protein -
  R8493_RS05715 (B10328_11100) - 1099129..1100019 (-) 891 WP_096568548.1 glycosyltransferase -
  R8493_RS05720 (B10328_11110) - 1100073..1100882 (-) 810 WP_317647066.1 glycosyltransferase family 2 protein -
  R8493_RS05725 (B10328_11120) waaF 1100941..1101891 (+) 951 WP_317647068.1 lipopolysaccharide heptosyltransferase II Regulator
  R8493_RS05730 (B10328_11130) - 1101877..1102641 (-) 765 WP_002825331.1 glycosyltransferase family 25 protein -
  R8493_RS05735 (B10328_11140) gmhA 1102641..1103201 (-) 561 WP_002787744.1 D-sedoheptulose 7-phosphate isomerase -
  R8493_RS05740 (B10328_11150) rfaE1 1103198..1104583 (-) 1386 WP_052804138.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  R8493_RS05745 (B10328_11160) rfaD 1104576..1105529 (-) 954 WP_002859254.1 ADP-glyceromanno-heptose 6-epimerase -
  R8493_RS05750 (B10328_11170) - 1105530..1106090 (-) 561 WP_396335582.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  R8493_RS05755 (B10328_11180) - 1106167..1106466 (+) 300 WP_002777238.1 cytochrome c -
  R8493_RS05760 (B10328_11190) ccoS 1106496..1106696 (-) 201 WP_317647070.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  R8493_RS05765 (B10328_11200) - 1106689..1109052 (-) 2364 WP_096568556.1 heavy metal translocating P-type ATPase -
  R8493_RS05770 (B10328_11210) rho 1109165..1110463 (+) 1299 WP_002780613.1 transcription termination factor Rho -
  R8493_RS05775 (B10328_11220) - 1110466..1112022 (+) 1557 WP_317647071.1 DNA polymerase III subunit gamma/tau -

Sequence

Protein


Download         Length: 316 a.a.        Molecular weight: 36151.73 Da        Isoelectric Point: 10.2421

>NTDB_id=104461 R8493_RS05725 WP_317647068.1 1100941..1101891(+) (waaF) [Campylobacter coli strain BCH-10328]
MKIFIHLPTWLGDAVMASPALYAIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKLSRYKQALSLRKELGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLGIKAHFKDLKLPFKLKFQSPLVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKIKTVAIFGPTKFTQTSPWQNENATLVHLDLACMPCMQKTCPLKHHKCMKDLKPEVIVKAIQNLINI

Nucleotide


Download         Length: 951 bp        

>NTDB_id=104461 R8493_RS05725 WP_317647068.1 1100941..1101891(+) (waaF) [Campylobacter coli strain BCH-10328]
ATGAAAATTTTTATACATCTTCCCACTTGGCTAGGCGATGCAGTGATGGCTTCACCTGCTTTATACGCTATAAAAGAACA
TTTTAAAAATGCCCAATTTATCCTTTATGGATCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACTATCCCGTTATAAACAAGCTCTATCTTTACGCAAAGAACTTGGTAAGATCGATTTTAGCTTTGCT
TTTAGGTCTGCGTTTTCTTCTAAGATTATCTTGCATATTCTTAAAACGAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTGGCATAAAAGCTCATTTTAAAGACTTAA
AGCTTCCCTTTAAGCTAAAATTTCAAAGCCCTCTTGTCTTAAAAAATGGCAAAAAAATTCTAGGACTCAACCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAGATTTT
AATCTTTGGCGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGACCTATGCACATAAGTGCGGTCTATAAGATAAAAACCGTGGCTATTTTTGGTCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAACGAAAATGCAACATTAGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAGCCTGAAGTGATTGTAAAAGCGATTCAAAATTTAATTAATATCTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(96-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

92.994

99.367

0.924

Multiple sequence alignment