Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACEN9H_RS27875 Genome accession   NZ_CP168562
Coordinates   6358946..6360448 (-) Length   500 a.a.
NCBI ID   WP_413672856.1    Uniprot ID   -
Organism   Massilia cellulosiltytica strain CT11-92     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6353946..6365448
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN9H_RS27845 (ACEN9H_27850) - 6354393..6354875 (+) 483 WP_259448148.1 PTS sugar transporter subunit IIA -
  ACEN9H_RS27850 (ACEN9H_27855) hprK 6354892..6355827 (+) 936 WP_036238906.1 HPr(Ser) kinase/phosphatase -
  ACEN9H_RS27855 (ACEN9H_27860) rapZ 6356015..6356878 (+) 864 WP_413672853.1 RNase adapter RapZ -
  ACEN9H_RS27860 (ACEN9H_27865) - 6356895..6357347 (-) 453 WP_413672854.1 SRPBCC family protein -
  ACEN9H_RS27865 (ACEN9H_27870) - 6357344..6358420 (-) 1077 WP_413672855.1 enoyl-CoA hydratase/isomerase family protein -
  ACEN9H_RS27870 (ACEN9H_27875) hpf 6358523..6358882 (-) 360 WP_036238902.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACEN9H_RS27875 (ACEN9H_27880) rpoN/rpoN1 6358946..6360448 (-) 1503 WP_413672856.1 RNA polymerase factor sigma-54 Machinery gene
  ACEN9H_RS27880 (ACEN9H_27885) lptB 6360451..6361227 (-) 777 WP_371765226.1 LPS export ABC transporter ATP-binding protein -
  ACEN9H_RS27885 (ACEN9H_27890) lptA 6361242..6361811 (-) 570 WP_413672857.1 lipopolysaccharide transport periplasmic protein LptA -
  ACEN9H_RS27890 (ACEN9H_27895) lptC 6361825..6362436 (-) 612 WP_056329249.1 LPS export ABC transporter periplasmic protein LptC -
  ACEN9H_RS27895 (ACEN9H_27900) - 6362444..6362977 (-) 534 WP_413672858.1 KdsC family phosphatase -
  ACEN9H_RS27900 (ACEN9H_27905) - 6362977..6363999 (-) 1023 WP_413672859.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 500 a.a.        Molecular weight: 54885.29 Da        Isoelectric Point: 4.7845

>NTDB_id=1044571 ACEN9H_RS27875 WP_413672856.1 6358946..6360448(-) (rpoN/rpoN1) [Massilia cellulosiltytica strain CT11-92]
MKQTLQLKTSQHLALTPQLQQSIRLLQLSTLELHQEIEQVLSENPLLERLDDPLDRSVRLLADGAINSAPASSQASEAPA
AQGNGEEGGSAPAENANGEANDAGGEGGEGGGEGGEDWGEYGRSSGSGGEDEDGRPQLEASPVTLREHLMEQVRMTCCAA
RDCALVELVVDALDDNGYLEEPLEDIHARLPEELEIEFDELRTALAMVQSMDPPGVGARSAAECLALQIRRMPGVPMVTR
RMALVIVEKYLTWFAQREFNKLKKALDCDDEDLREAQAVIRQCNPHPGAAFASDVSDFVVPDVIVRRAKDGWSVLLNPEV
MPRLRVNSLYASLMKQGKSETQMNAQLQEAKWLIKNMRQRFDTILRVAQAIVDRQRNFFSHGAVAMRPLVLREIADTLGL
HESTISRVTTQKYMLTPHGMFELKYFFGSHVATEAGGEASSTAIRALIVQLTGAEDPKNPLSDSKIAEMLGEQGMVIARR
TVAKYREALKIPPVSLRKSL

Nucleotide


Download         Length: 1503 bp        

>NTDB_id=1044571 ACEN9H_RS27875 WP_413672856.1 6358946..6360448(-) (rpoN/rpoN1) [Massilia cellulosiltytica strain CT11-92]
ATGAAACAGACGCTGCAACTCAAGACCTCACAGCACCTTGCACTGACGCCGCAACTGCAGCAATCGATCCGCCTGCTGCA
GTTGTCCACGCTCGAGCTGCACCAGGAAATCGAGCAGGTCCTGAGCGAGAACCCGCTGCTCGAACGGCTCGACGATCCGC
TCGACCGCTCCGTGCGGTTGCTCGCCGACGGCGCGATCAACAGCGCCCCGGCGTCATCGCAGGCAAGCGAGGCGCCGGCC
GCGCAGGGCAACGGCGAGGAAGGCGGCAGCGCGCCCGCCGAGAACGCCAATGGCGAAGCGAATGACGCCGGCGGCGAGGG
CGGAGAGGGCGGCGGCGAAGGCGGCGAGGACTGGGGCGAATACGGCCGCAGCAGCGGCAGCGGCGGCGAGGACGAGGACG
GCCGCCCGCAGCTGGAAGCGAGCCCCGTCACCCTGCGCGAGCATTTGATGGAACAGGTGCGCATGACGTGCTGCGCGGCG
CGCGACTGCGCGCTCGTGGAACTCGTCGTCGACGCGCTCGACGACAACGGCTACCTGGAAGAACCGCTGGAAGACATCCA
CGCGCGCCTGCCGGAAGAACTCGAGATCGAGTTCGACGAATTGCGCACGGCGCTCGCCATGGTGCAGAGCATGGATCCGC
CCGGCGTGGGCGCGCGCAGCGCGGCCGAGTGTCTGGCGCTGCAGATCCGCCGCATGCCCGGCGTGCCGATGGTGACGCGG
CGCATGGCGCTCGTCATCGTCGAAAAATACCTGACGTGGTTCGCGCAGCGCGAATTCAACAAGCTGAAGAAGGCGCTCGA
TTGCGACGACGAAGACCTGCGCGAAGCCCAGGCCGTCATCCGCCAGTGCAATCCGCATCCGGGCGCGGCGTTCGCGTCGG
ACGTGTCGGACTTCGTCGTGCCCGACGTGATCGTGCGCCGCGCCAAGGATGGCTGGAGCGTGCTGCTGAATCCGGAAGTG
ATGCCGCGCCTGCGCGTGAATAGCCTGTACGCGTCGCTGATGAAGCAGGGCAAATCGGAAACACAGATGAACGCGCAGTT
GCAGGAAGCCAAGTGGCTCATCAAGAACATGCGCCAGCGCTTCGACACGATCCTGCGTGTAGCCCAAGCAATTGTCGATC
GCCAGCGCAACTTTTTTTCCCATGGCGCAGTCGCGATGCGGCCCCTTGTTTTACGTGAGATAGCTGATACACTAGGTCTA
CACGAGAGCACAATTTCTCGAGTCACAACTCAGAAATATATGCTGACCCCGCACGGCATGTTCGAGTTGAAGTATTTCTT
CGGGAGTCACGTTGCCACCGAAGCGGGAGGCGAAGCATCGTCGACGGCAATACGGGCCCTCATCGTACAACTCACAGGAG
CAGAAGACCCCAAGAATCCATTATCCGATAGCAAGATCGCGGAGATGCTAGGGGAACAGGGAATGGTCATCGCGCGACGC
ACGGTCGCAAAATACCGTGAGGCGCTGAAAATCCCGCCCGTCAGTCTCCGCAAATCCTTGTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(139-330)

Sigma-54, DNA binding domain (Sigma54_DBD)

(342-498)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

58.09

100

0.596

Multiple sequence alignment