Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACEN9J_RS17400 Genome accession   NZ_CP168540
Coordinates   3779925..3781514 (-) Length   529 a.a.
NCBI ID   WP_261209303.1    Uniprot ID   A0A2E9BVW2
Organism   Variovorax sp. Varisp41 strain CT11-41     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3774925..3786514
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN9J_RS17375 (ACEN9J_17375) - 3775772..3776383 (+) 612 WP_057598366.1 cytochrome b -
  ACEN9J_RS17380 (ACEN9J_17380) - 3776380..3776964 (+) 585 WP_413761628.1 YceI family protein -
  ACEN9J_RS17385 (ACEN9J_17385) - 3776998..3777639 (+) 642 WP_413761629.1 YceI family protein -
  ACEN9J_RS17390 (ACEN9J_17390) - 3777662..3778306 (+) 645 WP_413761630.1 LysE family translocator -
  ACEN9J_RS17395 (ACEN9J_17395) - 3778316..3779914 (-) 1599 WP_413761631.1 phospholipase D family protein -
  ACEN9J_RS17400 (ACEN9J_17400) rpoN/rpoN1 3779925..3781514 (-) 1590 WP_261209303.1 RNA polymerase factor sigma-54 Machinery gene
  ACEN9J_RS17405 (ACEN9J_17405) lptB 3781545..3782339 (-) 795 WP_413761632.1 LPS export ABC transporter ATP-binding protein -
  ACEN9J_RS17410 (ACEN9J_17410) lptA 3782336..3783049 (-) 714 WP_057595580.1 lipopolysaccharide transport periplasmic protein LptA -
  ACEN9J_RS17415 (ACEN9J_17415) - 3783249..3783896 (-) 648 WP_413761633.1 thiol:disulfide interchange protein DsbA/DsbL -
  ACEN9J_RS17420 (ACEN9J_17420) - 3784085..3784849 (-) 765 WP_057595594.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58556.64 Da        Isoelectric Point: 4.6205

>NTDB_id=1044517 ACEN9J_RS17400 WP_261209303.1 3779925..3781514(-) (rpoN/rpoN1) [Variovorax sp. Varisp41 strain CT11-41]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAAREEFGLDAADTPAPREDTEREAESD
YAGAAPSTSTTSTETASSSEAEPALDSPEREPDWEGDGTVDMAPDDSEWGSDAPARNNSSTGDDERADATELARSQESLQ
SFLHRQALSLRLNENDSAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDELVHHFQVALGLLQSLEPAGVGARDLG
ECLAIQLRALADEDETEEEELVRKTALAICKQPMELLARRDFKRLATLTRTNEELVRSALQVIARLEPKPGRRFVDVERN
IVIPDVIVTKTGRGTNVKFRVMLNPEVMPRLRVHDIYAGALKSHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVSNAI
VERQKSYFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPFGTVELKYFFGSALGTETGGNASSTAVRALIKQF
VGSESVKKPLSDSQISEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=1044517 ACEN9J_RS17400 WP_261209303.1 3779925..3781514(-) (rpoN/rpoN1) [Variovorax sp. Varisp41 strain CT11-41]
ATGAAACAAGGCTTGTCGCTGCGCGTTTCGCAGCATCTCGCGCTGACGCCTCAGCTGCAGCAGTCGATCCGGCTGCTGCA
GCTCTCCACGCTCGAGCTGAGCCAGGAGGTCGAGCAGATGCTCGACGAGAACCCCTTCCTCGAGCGCACCGCCGAGGAAG
CCGCGCGCGAGGAATTCGGCCTCGACGCCGCCGACACGCCCGCGCCGCGCGAGGACACCGAGCGCGAGGCCGAGAGCGAC
TACGCGGGTGCCGCGCCCTCGACCTCGACCACGTCCACCGAGACCGCCTCGTCGTCCGAGGCCGAGCCGGCCCTCGACTC
GCCCGAGCGCGAGCCCGACTGGGAAGGCGACGGCACCGTCGACATGGCGCCCGACGACAGCGAGTGGGGCAGCGACGCGC
CCGCGCGCAACAACAGCAGCACCGGCGACGACGAGCGCGCCGATGCCACCGAGCTCGCGCGCAGCCAGGAATCGCTGCAG
TCCTTCCTGCACCGCCAGGCGCTGAGCCTGCGGCTCAACGAGAACGACAGCGCCGCGCTGCGCTTCCTGATCGAGTCGCT
CAACGACGACGGCTACCTCGAGGATTCGCTGCCGGCCCTGGCCTCGGGCCTCGCGGGCGACGACAACGACCAGTTCGACG
AGCTGGTGCACCACTTCCAGGTCGCGCTCGGCCTGCTGCAGAGCCTGGAGCCCGCGGGCGTGGGCGCGCGCGATCTCGGC
GAATGCCTCGCGATCCAGCTGCGCGCGCTGGCCGACGAAGACGAGACAGAGGAAGAGGAACTGGTGCGCAAGACCGCGCT
CGCCATCTGCAAGCAGCCGATGGAGCTGCTCGCGCGGCGCGACTTCAAGCGCCTGGCCACGCTCACGCGCACCAACGAGG
AGCTGGTGCGCTCGGCGCTGCAGGTCATCGCGCGCCTGGAGCCCAAGCCGGGCCGCCGCTTCGTCGACGTCGAGCGCAAC
ATCGTCATTCCCGACGTGATCGTCACCAAGACCGGCCGCGGCACCAACGTGAAGTTCCGCGTCATGCTCAACCCCGAGGT
GATGCCGCGGCTGCGCGTGCACGACATCTATGCCGGCGCGCTCAAGTCGCACAAGGGCGAGGGCAGCCAGGCGCTGTCGC
AGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGAGCAACGCCATC
GTCGAGCGGCAGAAGAGCTACTTCGTGCACGGCGAGCTCGCGATGCGCCCGCTGGTGCTGCGCGAGATCGCCGACGAGCT
CGGGCTGCACGAATCCACCATCTCGCGCGTGACCACCGCCAAGTACATGGCCACGCCCTTCGGAACGGTCGAGCTCAAGT
ACTTCTTCGGCTCGGCGCTGGGCACCGAGACCGGCGGCAATGCCTCGAGCACCGCGGTGCGCGCGCTGATCAAGCAGTTC
GTCGGTTCGGAGAGCGTGAAGAAGCCGCTGTCGGACAGCCAGATCTCCGAGATGCTCAAGGAGCAGGGCATCGAGTGCGC
GCGCCGCACCGTCGCCAAGTACCGCGAGGCGCTGCGCATCGCGCCCGCCAACCTGCGCAAGGCCCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(371-527)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2E9BVW2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.845

100

0.531

Multiple sequence alignment