Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACEN8U_RS12085 Genome accession   NZ_CP168531
Coordinates   47037..48554 (+) Length   505 a.a.
NCBI ID   WP_015001557.1    Uniprot ID   K0DI60
Organism   Paraburkholderia phenoliruptrix strain CT11-68     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 42037..53554
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN8U_RS12060 (ACEN8U_12055) kdsD 43156..44139 (+) 984 WP_013587053.1 arabinose 5-phosphate isomerase KdsD -
  ACEN8U_RS12065 (ACEN8U_12060) - 44139..44699 (+) 561 WP_013587052.1 KdsC family phosphatase -
  ACEN8U_RS12070 (ACEN8U_12065) lptC 44696..45307 (+) 612 WP_013587051.1 LPS export ABC transporter periplasmic protein LptC -
  ACEN8U_RS12075 (ACEN8U_12070) lptA 45328..45999 (+) 672 WP_310104997.1 lipopolysaccharide transport periplasmic protein LptA -
  ACEN8U_RS12080 (ACEN8U_12075) lptB 45996..46781 (+) 786 WP_013587049.1 LPS export ABC transporter ATP-binding protein -
  ACEN8U_RS12085 (ACEN8U_12080) rpoN/rpoN1 47037..48554 (+) 1518 WP_015001557.1 RNA polymerase factor sigma-54 Machinery gene
  ACEN8U_RS12090 (ACEN8U_12085) hpf 48726..49082 (+) 357 WP_006048027.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACEN8U_RS12095 (ACEN8U_12090) ptsN 49344..49859 (+) 516 WP_013587047.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACEN8U_RS12100 (ACEN8U_12095) hprK 49999..50967 (+) 969 WP_006048029.1 HPr(Ser) kinase/phosphatase -
  ACEN8U_RS12105 (ACEN8U_12100) rapZ 51071..51964 (+) 894 WP_310104990.1 RNase adapter RapZ -
  ACEN8U_RS12110 (ACEN8U_12105) - 52004..52639 (+) 636 WP_013587045.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 505 a.a.        Molecular weight: 55272.29 Da        Isoelectric Point: 5.0000

>NTDB_id=1044500 ACEN8U_RS12085 WP_015001557.1 47037..48554(+) (rpoN/rpoN1) [Paraburkholderia phenoliruptrix strain CT11-68]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAISQNPLLENEDDWIASPLRVAADGSLIAQAPGSATPPDQM
GGNGSSSSTSGSERSENGEPQGVDEYNGLSGDGNGDASQWNLDDYGRSGNASDDDDLPPLQIHESSTSLRDHLMAQLRVT
QAGQRDRALVTFLIESLDDDGYLAATLEEVLADLPEELEVDLDELNAALALLHSFDPAGVGARSASECLKLQLLRLEPSP
TRALALDIVAHYLELLAARDFTRLRKHLKASDDDLRDAHALIRSLEPFPGAAYGKAEADYVVPDIMVRKTAQGWQAELNP
EVVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKSFFVHGEIAMRPLVLREIA
DTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAENAKTPLSDSRIAELLAEQGF
VVARRTVAKYREALRIPAVNLRKSL

Nucleotide


Download         Length: 1518 bp        

>NTDB_id=1044500 ACEN8U_RS12085 WP_015001557.1 47037..48554(+) (rpoN/rpoN1) [Paraburkholderia phenoliruptrix strain CT11-68]
ATGAAAGCCAGCCTCCAACTCCGCCTATCGCAGCATCTTGCGTTGACCCCGCAACTGCAGCAGTCCATCCGGCTGCTTCA
GCTGTCTACGCTCGAACTGCAGCAGGAAGTGGCCACCGCGATCTCGCAAAACCCGCTCCTCGAGAACGAAGACGACTGGA
TCGCGAGCCCGCTTCGCGTGGCGGCCGACGGCTCGCTGATCGCGCAGGCGCCCGGCTCCGCCACCCCGCCCGACCAGATG
GGCGGCAACGGCTCGTCGTCGTCCACGAGCGGCAGCGAGCGCTCGGAGAACGGCGAGCCGCAAGGCGTGGACGAGTACAA
CGGCCTCTCTGGCGACGGCAACGGCGACGCGTCCCAATGGAATCTCGACGACTACGGCCGCTCCGGCAACGCCTCGGACG
ACGACGACCTGCCGCCGCTGCAAATTCACGAATCGAGTACTTCGCTGCGCGATCACCTGATGGCGCAATTGCGCGTCACG
CAGGCCGGTCAGCGCGACCGGGCGCTCGTCACTTTTCTGATCGAATCGCTCGACGACGACGGCTATCTCGCCGCCACCCT
CGAAGAAGTGCTCGCGGACCTGCCCGAAGAGCTGGAAGTCGACCTCGACGAACTGAATGCGGCGCTTGCGCTGCTGCACA
GCTTCGACCCAGCGGGCGTCGGGGCGCGCTCCGCGTCCGAGTGCCTGAAGCTGCAATTATTGCGGCTCGAGCCGTCGCCT
ACGCGCGCGCTCGCGCTCGACATCGTTGCCCATTATCTGGAACTGCTCGCCGCGCGCGATTTCACGCGCCTGCGCAAGCA
TCTGAAAGCAAGCGACGACGACCTGCGCGACGCCCACGCGCTCATCCGCTCGCTGGAACCATTCCCGGGCGCGGCCTACG
GCAAGGCCGAGGCGGATTACGTCGTGCCGGACATCATGGTGCGTAAAACCGCGCAGGGCTGGCAGGCCGAACTCAATCCC
GAAGTGGTGCCGAAACTGCGCATCAACCACCTGTACGCCAATATTTTGCGCAACAACCGGGGCGATCCCGGCAGCGGTTC
GTTGCGCCAGCAACTGCAGGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGTTTCGAAACAATCCTGCGTGTCGCGC
AGGCCATTGTCGAGCGTCAAAAGAGCTTTTTTGTGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGAGAAATTGCT
GATACGCTGGGCCTACACGAGTCAACTGTCTCGCGTGTGACAACCGGTAAGTACATGCTCACCCCGTTCGGGACGCTCGA
GTTCAAATACTTCTTCGGTTCGCATGTTTCGACGGACACGGGCGGCGCGGCGTCTTCAACGGCCATTCGCGCGCTCATCA
AGCAACTGATAGGAGCGGAAAACGCAAAAACGCCTCTCTCAGACAGCCGCATAGCCGAACTGCTGGCGGAACAGGGCTTC
GTGGTCGCACGGCGCACCGTGGCGAAGTATCGCGAAGCGCTCAGGATTCCGGCAGTCAACCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(141-332)

Sigma-54, DNA binding domain (Sigma54_DBD)

(348-503)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K0DI60

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.601

100

0.671

Multiple sequence alignment