Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   ACEPOT_RS10300 Genome accession   NZ_CP168499
Coordinates   2152345..2153415 (-) Length   356 a.a.
NCBI ID   WP_000776422.1    Uniprot ID   -
Organism   Staphylococcus aureus strain J2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2147345..2158415
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPOT_RS10260 (ACEPOT_10260) gcvPA 2147522..2148868 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  ACEPOT_RS10265 (ACEPOT_10265) gcvT 2148888..2149979 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  ACEPOT_RS10270 (ACEPOT_10270) - 2150138..2150662 (-) 525 WP_001015121.1 shikimate kinase -
  ACEPOT_RS10275 (ACEPOT_10275) - 2150652..2150798 (-) 147 WP_001789879.1 hypothetical protein -
  ACEPOT_RS10280 (ACEPOT_10280) comGF 2150895..2151392 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACEPOT_RS10285 (ACEPOT_10285) comGE 2151310..2151609 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  ACEPOT_RS10290 (ACEPOT_10290) comGD 2151596..2152042 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACEPOT_RS10295 (ACEPOT_10295) comGC 2152020..2152331 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  ACEPOT_RS10300 (ACEPOT_10300) comGB 2152345..2153415 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACEPOT_RS10305 (ACEPOT_10305) comGA 2153387..2154361 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  ACEPOT_RS10310 (ACEPOT_10310) - 2154413..2155036 (-) 624 WP_001223010.1 MBL fold metallo-hydrolase -
  ACEPOT_RS10315 (ACEPOT_10315) - 2155033..2155362 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ACEPOT_RS10320 (ACEPOT_10320) - 2155362..2156348 (-) 987 WP_000161314.1 ROK family glucokinase -
  ACEPOT_RS10325 (ACEPOT_10325) - 2156345..2156548 (-) 204 WP_000087561.1 YqgQ family protein -
  ACEPOT_RS10330 (ACEPOT_10330) - 2156529..2157992 (-) 1464 WP_001017865.1 rhomboid family intramembrane serine protease -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41447.84 Da        Isoelectric Point: 10.0606

>NTDB_id=1044387 ACEPOT_RS10300 WP_000776422.1 2152345..2153415(-) (comGB) [Staphylococcus aureus strain J2]
MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1044387 ACEPOT_RS10300 WP_000776422.1 2152345..2153415(-) (comGB) [Staphylococcus aureus strain J2]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGATTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGTACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATCAAATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATTATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTTTCTATCTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAATTTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTGCCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGGAAGCTAGAAGTAGAACTAAAGTTATATTCACAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

100

100

1
  comGB Staphylococcus aureus N315

100

100

1

Multiple sequence alignment