Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   ACCK33_RS00200 Genome accession   NZ_CP168442
Coordinates   47774..49123 (-) Length   449 a.a.
NCBI ID   WP_138642932.1    Uniprot ID   -
Organism   Staphylococcus aureus strain J03     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 39359..54402 47774..49123 within 0


Gene organization within MGE regions


Location: 39359..54402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCK33_RS00160 (ACCK33_00160) mecA 39359..41368 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  ACCK33_RS00165 (ACCK33_00165) - 41465..42451 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  ACCK33_RS00170 (ACCK33_00170) - 42438..42683 (+) 246 Protein_31 type I restriction enzyme endonuclease domain-containing protein -
  ACCK33_RS00175 (ACCK33_00175) - 42674..44284 (-) 1611 Protein_32 IS1182 family transposase -
  ACCK33_RS00180 (ACCK33_00180) - 44333..44839 (-) 507 WP_001092189.1 DUF1643 domain-containing protein -
  ACCK33_RS00185 (ACCK33_00185) - 44854..45165 (-) 312 WP_000700851.1 DUF960 family protein -
  ACCK33_RS00190 (ACCK33_00190) - 45252..45602 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  ACCK33_RS00195 (ACCK33_00195) ccrB 46124..47752 (-) 1629 WP_001186608.1 cassette chromosome recombinase CcrB Machinery gene
  ACCK33_RS00200 (ACCK33_00200) ccrA 47774..49123 (-) 1350 WP_138642932.1 cassette chromosome recombinase CcrA Machinery gene
  ACCK33_RS00205 (ACCK33_00205) cch1 49357..51150 (-) 1794 WP_031806891.1 cassette chromosome replicative helicase -
  ACCK33_RS00210 (ACCK33_00210) ssb 51150..51443 (-) 294 WP_001587828.1 cassette chromosome ssDNA-binding protein -
  ACCK33_RS00215 (ACCK33_00215) - 51678..52817 (+) 1140 Protein_40 IS3 family transposase -
  ACCK33_RS00220 (ACCK33_00220) - 52888..54402 (-) 1515 WP_001587831.1 hypothetical protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52601.13 Da        Isoelectric Point: 9.8792

>NTDB_id=1044151 ACCK33_RS00200 WP_138642932.1 47774..49123(-) (ccrA) [Staphylococcus aureus strain J03]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGQLITTHAPFGYEYH
NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRLQKQTKQTSSDNQLKQKINCPCCNATLTNMTVRKKNHILRYYVCPKNMNASRFVCDFKGINAQTFEDKVLEV
CRDFYQNQRIYTKIKGAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHTIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=1044151 ACCK33_RS00200 WP_138642932.1 47774..49123(-) (ccrA) [Staphylococcus aureus strain J03]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGTCGCAAAGATAATCGTAGTGGGT
ATCGACAAATAACACAATTAATTCAACAAGGACAGTGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACGTACCATGTTCATATCTTAAGCGTACATGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGACTCAAGCTTAATATCTTCATCAGTTTAGCCGAACTTGAATCGGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCAATTGATTACAACACATGCACCTTTTGGTTACGAATATCAC
AACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATTGCAAAAACAAACCAAACAGACATCTTCGGATAATCAACTCAAACAAAAAATCAA
TTGCCCATGTTGTAATGCAACACTTACAAATATGACCGTTAGAAAAAAGAATCATATATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTATGTGATTTCAAAGGCATCAATGCACAAACATTTGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAGGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATCGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAACAACCTCAACGCACTACATCTATCAATGGACATCAAATACAACACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATCGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(169-248)

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

97.55

100

0.976
  ccrA Staphylococcus aureus COL

74.833

100

0.748

Multiple sequence alignment