Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACEPWQ_RS20300 Genome accession   NZ_CP168388
Coordinates   4264208..4265641 (+) Length   477 a.a.
NCBI ID   WP_032614481.1    Uniprot ID   A0A3E1ZUH7
Organism   Leclercia adecarboxylata strain LN01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4259208..4270641
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPWQ_RS20270 (ACEPWQ_20270) - 4259754..4260731 (+) 978 WP_032614470.1 calcium/sodium antiporter -
  ACEPWQ_RS20275 (ACEPWQ_20275) kdsD 4260745..4261731 (+) 987 WP_144838499.1 arabinose-5-phosphate isomerase KdsD -
  ACEPWQ_RS20280 (ACEPWQ_20280) kdsC 4261746..4262312 (+) 567 WP_032614474.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACEPWQ_RS20285 (ACEPWQ_20285) lptC 4262309..4262884 (+) 576 WP_032614475.1 LPS export ABC transporter periplasmic protein LptC -
  ACEPWQ_RS20290 (ACEPWQ_20290) lptA 4262853..4263428 (+) 576 WP_032614476.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACEPWQ_RS20295 (ACEPWQ_20295) lptB 4263435..4264160 (+) 726 WP_032614478.1 LPS export ABC transporter ATP-binding protein -
  ACEPWQ_RS20300 (ACEPWQ_20300) rpoN/rpoN1 4264208..4265641 (+) 1434 WP_032614481.1 RNA polymerase factor sigma-54 Machinery gene
  ACEPWQ_RS20305 (ACEPWQ_20305) hpf 4265664..4265951 (+) 288 WP_032614483.1 ribosome hibernation promoting factor -
  ACEPWQ_RS20310 (ACEPWQ_20310) ptsN 4266034..4266525 (+) 492 WP_032614485.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACEPWQ_RS20315 (ACEPWQ_20315) rapZ 4266571..4267425 (+) 855 WP_032614487.1 RNase adapter RapZ -
  ACEPWQ_RS20320 (ACEPWQ_20320) npr 4267422..4267694 (+) 273 WP_032614489.1 PTS phosphocarrier protein NPr -
  ACEPWQ_RS20325 (ACEPWQ_20325) mtgA 4267691..4268416 (-) 726 WP_373407757.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACEPWQ_RS20330 (ACEPWQ_20330) elbB 4268413..4269066 (-) 654 WP_077226308.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54186.01 Da        Isoelectric Point: 4.3155

>NTDB_id=1043912 ACEPWQ_RS20300 WP_032614481.1 4264208..4265641(+) (rpoN/rpoN1) [Leclercia adecarboxylata strain LN01]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVETQQTQDNETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLDD
ILESIGDDEIDLEEVEAVLKRVQRFDPIGVAARDLRDCLLIQLSQFVKETPWLEEARLIVSEHLDLLANHDFRSLMRVTR
LKEEILKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGLWTVELNSDSIPRLQINQQYASMCTSSRNDSDNQFIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=1043912 ACEPWQ_RS20300 WP_032614481.1 4264208..4265641(+) (rpoN/rpoN1) [Leclercia adecarboxylata strain LN01]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCAATGACGCCGCAGTTACAACAGGCCATTCGTCTGTTGCA
GCTGTCGACGCTAGAACTTCAGCAGGAGCTTCAGCAAGCGCTGGACAGTAATCCCCTGCTTGAGCAAACCGATCTTCACG
ACGAGGTAGAAACCCAGCAAACGCAGGATAACGAAACGCTCGACAGCGTCGACGCGCTGGAGCAAAAAGAGATGCCAGAC
GAGCTTCCTCTTGATGCCAGCTGGGATGAGATCTACACCGCCGGCACGCCATCCGGTACGCGCGCTGATTACCAGGATGA
TGAGTTGCCGGTGTATCAGGGCGAAACCACCCAGTCCCTCCAGGATTACCTGATGTGGCAGGTGGAGCTAACCCCGTTTT
CCGATACCGATCGCGCCATTGCCACCTCCATTGTCGATGCCGTCGATGAGACCGGCTATTTAACCGTGCCGCTGGACGAC
ATTCTGGAAAGCATCGGTGATGATGAGATCGATCTTGAAGAGGTCGAAGCGGTCCTGAAGCGCGTGCAGCGTTTTGATCC
GATTGGCGTTGCGGCACGTGATTTACGTGACTGCCTGCTGATTCAGCTTTCGCAGTTCGTCAAAGAGACGCCGTGGCTGG
AAGAGGCTCGTCTGATCGTCAGCGAGCATCTGGATCTGCTGGCCAACCACGACTTCCGCTCGTTGATGCGGGTGACGCGA
TTGAAAGAAGAGATCCTGAAAGAGGCGGTCAATCTTATTCAGTCGTTGGATCCGCGTCCGGGGCAATCCATCCAGACAAG
CGAGCCTGAGTACGTTATTCCCGATGTACTGGTACGCAAGCATAACGGCCTGTGGACCGTGGAGCTGAATTCCGACAGCA
TCCCGCGCCTGCAAATTAATCAGCAATACGCCTCGATGTGCACCAGTTCGCGCAACGACTCGGATAACCAGTTTATCCGC
AGCAACCTGCAGGAGGCGCGCTGGCTGATCAAAAGCCTCGAGAGCCGCAATGATACGCTGCTACGCGTGAGCCGCTGCAT
CGTGGAGCAGCAGCAGGCCTTCTTTGAACAAGGTGAAGAATTTATGAAGCCGATGGTGCTGGCCGATATTGCCCAGGCCG
TCGAAATGCATGAATCAACGATTTCCCGCGTGACGACGCAGAAGTATCTGCACAGCCCGCGCGGTATTTTTGAGCTTAAG
TATTTTTTCTCCAGCCATGTCAATACCGAAGGCGGTGGCGAAGCTTCGTCTACAGCCATCCGGGCGCTGGTGAAAAAATT
GATCGCCGCGGAAAACCCCGCGAAACCACTGAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAAGGTATCATGGTGG
CACGCCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3E1ZUH7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment