Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   R8575_RS06400 Genome accession   NZ_AP028336
Coordinates   1221186..1222145 (+) Length   319 a.a.
NCBI ID   WP_317653969.1    Uniprot ID   -
Organism   Campylobacter jejuni strain BCH-10190     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1200856..1234365 1221186..1222145 within 0


Gene organization within MGE regions


Location: 1200856..1234365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8575_RS06285 (B10190_12350) pglI 1200856..1201785 (-) 930 WP_032583777.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  R8575_RS06290 (B10190_12360) pglH 1201778..1202857 (-) 1080 WP_002859237.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  R8575_RS06295 (B10190_12370) pglK 1202854..1204548 (-) 1695 WP_002867213.1 ABC-type lipopolysaccharide transporter PglK -
  R8575_RS06300 (B10190_12380) galE 1204542..1205528 (-) 987 WP_032587260.1 UDP-glucose 4-epimerase GalE -
  R8575_RS06305 (B10190_12390) - 1205587..1206381 (-) 795 WP_038813904.1 3'-5' exonuclease -
  R8575_RS06310 (B10190_12400) waaC 1206448..1207476 (+) 1029 WP_317653966.1 lipopolysaccharide heptosyltransferase I -
  R8575_RS06315 (B10190_12410) htrB 1207466..1208344 (+) 879 WP_100624280.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  R8575_RS06320 (B10190_12420) - 1208341..1209042 (+) 702 WP_002876132.1 glycosyltransferase family 2 protein -
  R8575_RS06325 (B10190_12430) rfbA 1209053..1209931 (+) 879 WP_002869595.1 glucose-1-phosphate thymidylyltransferase RfbA -
  R8575_RS06330 (B10190_12440) rfbB 1209931..1210962 (+) 1032 WP_100647782.1 dTDP-glucose 4,6-dehydratase -
  R8575_RS06335 (B10190_12450) wlaRA 1210965..1211414 (+) 450 WP_002912325.1 class E lipooligosaccharide biosynthesis 3,4-ketoisomerase WlaRA -
  R8575_RS06340 (B10190_12460) wlaRB 1211411..1211815 (+) 405 WP_002860228.1 class E lipooligosaccharide biosynthesis 3,4-ketoisomerase WlaRB -
  R8575_RS06345 (B10190_12470) - 1211805..1212248 (+) 444 WP_002860229.1 N-acetyltransferase -
  R8575_RS06350 (B10190_12480) - 1212270..1212797 (+) 528 WP_002876185.1 GNAT family N-acetyltransferase -
  R8575_RS06355 - 1212825..1213076 (+) 252 Protein_1239 hypothetical protein -
  R8575_RS06360 (B10190_12490) - 1213095..1214171 (+) 1077 WP_038402147.1 glycosyltransferase family 2 protein -
  R8575_RS06365 (B10190_12500) wlaRG 1214168..1215253 (+) 1086 WP_038402148.1 class E lipooligosaccharide biosynthesis aminotransferase WlaRG -
  R8575_RS06370 (B10190_12510) - 1215285..1216538 (-) 1254 WP_100647785.1 DUF2972 domain-containing protein -
  R8575_RS06375 (B10190_12520) - 1216535..1217557 (-) 1023 WP_002891861.1 hypothetical protein -
  R8575_RS06380 (B10190_12530) - 1217628..1218401 (+) 774 WP_002891857.1 glycosyltransferase family 25 protein -
  R8575_RS06385 (B10190_12540) wlaTB 1218394..1219350 (+) 957 WP_002860237.1 class E lipooligosaccharide biosynthesis protein WlaTB -
  R8575_RS06390 (B10190_12550) wlaTC 1219347..1220306 (+) 960 WP_002860238.1 class E lipooligosaccharide biosynthesis protein WlaTC -
  R8575_RS06395 (B10190_12560) - 1220309..1221127 (-) 819 WP_002876193.1 glycosyltransferase family 2 protein -
  R8575_RS06400 (B10190_12570) waaF 1221186..1222145 (+) 960 WP_317653969.1 lipopolysaccharide heptosyltransferase II Regulator
  R8575_RS06405 (B10190_12580) gmhA 1222126..1222686 (-) 561 WP_002876196.1 D-sedoheptulose 7-phosphate isomerase -
  R8575_RS06410 (B10190_12590) rfaE1 1222683..1224068 (-) 1386 WP_002901184.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  R8575_RS06415 (B10190_12600) rfaD 1224061..1225014 (-) 954 WP_002860242.1 ADP-glyceromanno-heptose 6-epimerase -
  R8575_RS06420 (B10190_12610) gmhB 1225015..1225576 (-) 562 Protein_1252 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  R8575_RS06425 (B10190_12620) - 1225657..1225959 (+) 303 WP_002852762.1 c-type cytochrome -
  R8575_RS06430 (B10190_12630) ccoS 1225982..1226188 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  R8575_RS06435 (B10190_12640) - 1226185..1228542 (-) 2358 WP_317653970.1 heavy metal translocating P-type ATPase -
  R8575_RS06440 (B10190_12650) rho 1228651..1229949 (+) 1299 WP_002852852.1 transcription termination factor Rho -
  R8575_RS06445 (B10190_12660) dnaX 1229953..1231482 (+) 1530 WP_002878814.1 DNA polymerase III subunit gamma/tau -
  R8575_RS06450 (B10190_12670) - 1231479..1232000 (-) 522 WP_317653972.1 DUF2165 family protein -
  R8575_RS06455 (B10190_12680) copA 1232072..1234171 (-) 2100 WP_317653974.1 copper-translocating P-type ATPase CopA -
  R8575_RS06460 (B10190_12690) - 1234171..1234365 (-) 195 WP_002852760.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 319 a.a.        Molecular weight: 36635.13 Da        Isoelectric Point: 10.2870

>NTDB_id=104389 R8575_RS06400 WP_317653969.1 1221186..1222145(+) (waaF) [Campylobacter jejuni strain BCH-10190]
MKIFIHLPTWLGDAVMVSPALYAIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKELGKIDLSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLSIKAHSKDLKLPFKLKLQDPLVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKSEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKVKTVAIFGPTKFTQTSPWQNENAILVHLDLACMPCMQKTCPLKHHKCMKDLKPQRVIEEARNLLKNSHR

Nucleotide


Download         Length: 960 bp        

>NTDB_id=104389 R8575_RS06400 WP_317653969.1 1221186..1222145(+) (waaF) [Campylobacter jejuni strain BCH-10190]
ATGAAAATTTTTATACATCTTCCCACTTGGCTGGGCGATGCAGTGATGGTTTCACCTGCTTTATACGCTATAAAAGAACA
TTTTAAAAATGCCCAATTTATCCTTTATGGCTCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCTCGCTATAAACAAGCCCTATCTTTACGCAAAGAACTTGGTAAGATCGATCTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTGCATATCCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTAGTATAAAAGCTCATTCTAAAGACTTAA
AGCTTCCCTTTAAGCTAAAACTTCAAGACCCTCTTGTCTTAAAAAATGGTAAAAAAATTCTAGGACTCAATCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAAATTTT
AATCTTTGGCGCAGGAAAATCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAACAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGTCCTATGCACATAAGTGCGGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATGAAAACGCAATATTAGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATCTAAAGCCTCAAAGGGTTATAGAAGAGGCTAGAAATTTACTTAAAAACTCTCATCGATGA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(94-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

93.711

99.687

0.934

Multiple sequence alignment