Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACEI37_RS12745 Genome accession   NZ_CP168303
Coordinates   361702..363180 (-) Length   492 a.a.
NCBI ID   WP_373379182.1    Uniprot ID   -
Organism   Cupriavidus nantongensis strain HB4B5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 356702..368180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEI37_RS12715 (ACEI37_12715) - 357139..357798 (-) 660 WP_373380251.1 LON peptidase substrate-binding domain-containing protein -
  ACEI37_RS12720 (ACEI37_12720) rapZ 357890..358768 (-) 879 WP_373379179.1 RNase adapter RapZ -
  ACEI37_RS12725 (ACEI37_12725) - 358884..359276 (-) 393 WP_373379180.1 PsiF family protein -
  ACEI37_RS12730 (ACEI37_12730) hprK 359448..360419 (-) 972 WP_012351664.1 HPr(Ser) kinase/phosphatase -
  ACEI37_RS12735 (ACEI37_12735) - 360502..360957 (-) 456 WP_373379181.1 PTS sugar transporter subunit IIA -
  ACEI37_RS12740 (ACEI37_12740) hpf 361303..361653 (-) 351 WP_010814386.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACEI37_RS12745 (ACEI37_12745) rpoN/rpoN1 361702..363180 (-) 1479 WP_373379182.1 RNA polymerase factor sigma-54 Machinery gene
  ACEI37_RS12750 (ACEI37_12750) lptB 363304..364104 (-) 801 WP_115708053.1 LPS export ABC transporter ATP-binding protein -
  ACEI37_RS12755 (ACEI37_12755) lptA 364113..364757 (-) 645 WP_373379183.1 lipopolysaccharide transport periplasmic protein LptA -
  ACEI37_RS12760 (ACEI37_12760) lptC 364874..365503 (-) 630 WP_111518221.1 LPS export ABC transporter periplasmic protein LptC -
  ACEI37_RS12765 (ACEI37_12765) - 365550..366533 (-) 984 WP_373379184.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 492 a.a.        Molecular weight: 54662.96 Da        Isoelectric Point: 4.9633

>NTDB_id=1043770 ACEI37_RS12745 WP_373379182.1 361702..363180(-) (rpoN/rpoN1) [Cupriavidus nantongensis strain HB4B5]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNLQSAPAPAPAEPQ
GNGDARAEGAADDNYSDNSSGDDYGSGDWSLDDFARRPQGDEDEKTPMQLRDAEPTLREYLMEQLTPLKISARDKGLAIF
LIESLDDDGYLSASLEEICAELPEELEFDPDEVHAILTLLQSFDPPGVGARNAAECLALQLRRLTHPQRELALTIVTHHL
ELLAVRDYTRLKKALQVDEVALKAAHDLIRSLAPYPGHAYSRPEADFVVPDVFVRKSGGGWIAQLNPDVMPRLRINDMYA
QILRGAKGETGTAGLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKNFFTHGEIAMRPLVLREIADTLGLHESTISRV
TTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPRNPLSDSRIAELLGEQGFVVARRTVAKYREA
LKIPAVNLRKSL

Nucleotide


Download         Length: 1479 bp        

>NTDB_id=1043770 ACEI37_RS12745 WP_373379182.1 361702..363180(-) (rpoN/rpoN1) [Cupriavidus nantongensis strain HB4B5]
ATGAAACCGTCGCTACAGCTCCGCCTCTCCCAGCACCTGGCACTGACCCCGCAGCTGCAACAGTCGATCCGGCTGCTGCA
GCTTTCCACGCTGGAATTGCAGCAGGAAGTCGAACAGGCGCTGACGGAAAACCCGCTGCTCGAGCGGGAGAACGACTGGA
TCGAAAGCCCGCTGCGCGTGGCTGCCGATGGCTCGGTCAACCTCCAGAGCGCGCCAGCGCCCGCCCCCGCCGAGCCCCAG
GGCAATGGCGACGCACGCGCCGAAGGCGCCGCCGATGACAACTACAGCGACAACAGCAGCGGCGACGATTATGGCAGCGG
CGACTGGAGCCTGGACGATTTCGCCCGCCGGCCGCAAGGCGACGAGGACGAAAAGACGCCAATGCAGCTGCGCGATGCCG
AGCCCACGCTGCGCGAATACCTGATGGAGCAGCTGACGCCGCTGAAGATCTCGGCGCGTGACAAGGGCCTCGCCATCTTC
CTGATCGAATCGCTCGATGACGATGGCTACCTGAGCGCCTCGCTCGAAGAGATCTGCGCGGAACTGCCGGAAGAACTCGA
GTTCGACCCCGACGAAGTCCACGCCATCCTGACGCTGCTGCAGAGCTTCGATCCGCCCGGCGTGGGCGCGCGCAATGCCG
CCGAATGCCTGGCGCTGCAATTGCGCCGCCTGACGCACCCGCAGCGCGAACTGGCGCTGACCATCGTCACGCATCACCTG
GAGCTGCTGGCGGTACGCGACTACACGCGGCTGAAGAAAGCCCTGCAGGTCGATGAAGTCGCGCTCAAGGCCGCGCATGA
CCTGATCCGCTCGCTGGCACCGTACCCAGGCCATGCGTACAGCCGCCCCGAGGCCGATTTCGTCGTGCCGGATGTGTTCG
TGCGCAAGAGCGGCGGCGGCTGGATCGCGCAACTCAATCCGGATGTGATGCCGAGGCTGCGCATCAACGATATGTATGCC
CAGATCCTGCGTGGCGCCAAGGGCGAGACCGGTACCGCCGGACTGCAGCAAAAGCTGCAAGAGGCACGGTGGCTGATCAA
GAATATCCAGCAAAGGTTCGACACGATCCTGCGTGTCTCGCAGGCCATTGTCGAGCGTCAAAAGAACTTTTTCACCCACG
GTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAGATTGCCGATACACTGGGTTTACACGAGTCAACCATCTCCCGGGTG
ACGACCAATAAATACATGGCCACGCCGATGGGTACTTTCGAACTGAAGTACTTCTTTGGCAGCCACGTGTCCACCGAAAC
CGGTGGCGCGGCTTCATCAACGGCCATCCGCGCCTTGATCAAGCAACTGATAGGAGCCGAAGACCCGAGGAATCCCCTTT
CCGACAGTCGCATCGCCGAACTGCTGGGCGAGCAGGGCTTCGTTGTCGCACGCCGAACCGTCGCCAAATATCGTGAAGCC
TTGAAAATCCCCGCAGTCAATCTCCGCAAGTCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(335-490)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-319)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

79.4

100

0.807

Multiple sequence alignment