Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   P6F15_RS03290 Genome accession   NZ_CP168247
Coordinates   681053..683272 (-) Length   739 a.a.
NCBI ID   WP_373332465.1    Uniprot ID   -
Organism   Thiopseudomonas alkaliphila strain 177     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 676053..688272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P6F15_RS03275 (P6F15_03275) - 677939..678418 (-) 480 WP_373332464.1 NYN domain-containing protein -
  P6F15_RS03280 (P6F15_03280) - 678516..679220 (-) 705 WP_053107954.1 SPOR domain-containing protein -
  P6F15_RS03285 (P6F15_03285) argS 679224..680966 (-) 1743 WP_053100011.1 arginine--tRNA ligase -
  P6F15_RS03290 (P6F15_03290) priA 681053..683272 (-) 2220 WP_373332465.1 primosomal protein N' Machinery gene
  P6F15_RS03295 (P6F15_03295) - 683345..684646 (-) 1302 WP_053107952.1 glutathionylspermidine synthase family protein -
  P6F15_RS03300 (P6F15_03300) - 684650..685198 (-) 549 WP_053100007.1 hypothetical protein -
  P6F15_RS03305 (P6F15_03305) - 685305..685985 (-) 681 WP_373332466.1 lytic transglycosylase domain-containing protein -
  P6F15_RS03310 (P6F15_03310) - 686111..686569 (-) 459 WP_053105875.1 NUDIX hydrolase -
  P6F15_RS03315 (P6F15_03315) icd 686931..688184 (+) 1254 WP_373332467.1 NADP-dependent isocitrate dehydrogenase -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 82211.38 Da        Isoelectric Point: 8.3535

>NTDB_id=1043455 P6F15_RS03290 WP_373332465.1 681053..683272(-) (priA) [Thiopseudomonas alkaliphila strain 177]
MSALYLRLALPSPLRRLFDYLPGNAVDPMQLQPGIRIKVPFANRELIGILVEVVSHSEVPQNKLKPIVAVLDQTPLIPKS
LFSLCQWSAKYYHHSLGDTFNWALPVLLRQGEAASKKPSLFWHALPEAQVTDPAIAKAPKQQHALAILLQHPKGLAHTQL
GQFGLSKDLLDKLASKQLAVCREQVFNLAQKPCPTLAQPELTLNEQQQAALAAILDKLQQFQVFLLAGVTGSGKTEVYLQ
AIKHCLAQGQQVLVLIPEINLGPQTIQRFSQRFKAKIALLNSEVNDRQRLDGWIEANSGEADIVIGTRSALFTPMPNLGL
IIIDEEHDSSYKQQEGLRYHARDVAIVRAMQAKIPIVLGSATPSLESLYNAEQGRYTLLKLTQRAGQAAEPSFIRLDIKH
LPLDAGISQALQKEMRQTLAAGQQVLVFLNRRGFAPVLMCHDCGWISHCHACDARLTLHQRYNELRCHHCGHIEPRPHHC
PKCQHLDLRPIGSGTERTEERLAIMFPDTPVLRIDRDSTARKGALQGMLEIINQGQPCILLGTQMLAKGHHFPKVTLVAI
LNADGGLFSADFRASEYMAQQILQVAGRAGRAAEPGRVIIQSHLADHPLLVQLTEQGYFAFAKQALEERRHALMPPLSHL
VLLRAESVKPEQGHLFLEQAHALAEQLQLSLDCPEVELLGPVPAPMERRQGRHRAQLLLLSTQRTQLHCLVNALVHNLEP
LPSARSVRWSVDVDPIELF

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=1043455 P6F15_RS03290 WP_373332465.1 681053..683272(-) (priA) [Thiopseudomonas alkaliphila strain 177]
GTGTCTGCTTTGTATCTGCGTCTTGCTTTACCTTCACCGTTGCGACGGCTCTTTGATTACTTACCGGGTAATGCCGTAGA
TCCCATGCAGTTACAACCAGGCATTAGAATCAAGGTTCCCTTTGCTAACCGCGAGCTGATTGGCATTTTGGTTGAAGTGG
TTAGCCACAGCGAAGTGCCACAAAATAAACTAAAACCGATTGTGGCTGTGCTCGATCAAACGCCGCTTATTCCCAAAAGC
TTATTTAGCCTCTGCCAATGGAGCGCCAAGTATTATCACCACAGCCTCGGTGATACCTTTAACTGGGCGTTGCCGGTGTT
ACTGCGCCAAGGCGAAGCCGCCAGCAAAAAACCTAGTCTCTTCTGGCACGCCCTCCCCGAGGCCCAGGTTACCGATCCTG
CCATTGCTAAAGCACCCAAACAGCAGCACGCCCTTGCGATCCTATTGCAGCACCCCAAAGGTCTTGCCCATACGCAACTG
GGGCAATTTGGGTTAAGCAAAGACTTGCTCGATAAACTGGCCAGCAAACAGCTTGCCGTCTGCCGCGAGCAAGTATTTAA
CCTGGCGCAAAAGCCATGTCCAACGCTTGCCCAACCAGAGTTAACGCTCAATGAGCAGCAACAAGCCGCTTTAGCGGCTA
TTTTAGATAAGCTGCAGCAGTTTCAAGTGTTTTTATTGGCCGGGGTGACCGGAAGCGGTAAAACCGAAGTTTATCTACAA
GCAATTAAACATTGCTTGGCCCAAGGCCAGCAAGTGCTGGTGTTAATTCCAGAGATTAACTTAGGCCCACAAACCATTCA
GCGTTTTAGCCAACGCTTTAAGGCCAAGATTGCTCTGCTTAATTCTGAAGTGAATGATCGCCAGCGTTTAGACGGTTGGA
TCGAAGCGAACAGCGGTGAGGCGGATATTGTCATTGGTACCCGCTCGGCGCTGTTTACTCCAATGCCCAACTTAGGCCTG
ATTATTATTGATGAAGAGCACGACAGCTCCTATAAGCAGCAAGAAGGCTTACGCTATCACGCCCGTGATGTGGCGATTGT
GCGGGCCATGCAAGCCAAGATTCCAATTGTTTTAGGCTCGGCGACCCCATCCCTAGAAAGCCTCTACAATGCCGAACAAG
GCCGCTATACGTTACTCAAGCTAACGCAACGCGCTGGCCAAGCGGCTGAGCCTAGTTTTATTCGGTTAGATATTAAACAC
TTGCCACTGGATGCGGGTATTTCCCAAGCGCTGCAAAAAGAGATGCGTCAAACTTTAGCTGCAGGCCAACAAGTGCTGGT
GTTTTTAAACCGCCGTGGCTTTGCTCCAGTCTTGATGTGCCACGACTGCGGTTGGATTAGCCACTGCCACGCCTGCGATG
CTCGCTTAACCTTGCATCAACGCTATAACGAATTACGCTGTCATCACTGCGGTCATATCGAGCCACGCCCACACCACTGC
CCTAAGTGCCAACATCTAGACTTACGCCCGATTGGCAGCGGCACTGAACGCACCGAAGAACGCCTAGCCATTATGTTTCC
AGATACTCCGGTGTTACGCATTGATCGAGACAGCACCGCACGCAAAGGCGCTCTGCAAGGCATGCTTGAAATTATTAACC
AAGGCCAACCCTGTATTTTGCTAGGCACGCAAATGCTGGCTAAAGGCCATCACTTTCCGAAAGTGACCTTGGTGGCAATT
TTAAATGCCGATGGTGGATTATTTTCTGCCGATTTTCGCGCCAGTGAATACATGGCGCAACAGATTCTGCAAGTTGCAGG
ACGCGCCGGACGCGCGGCTGAACCGGGCCGGGTGATTATCCAAAGCCACCTTGCCGACCATCCGCTATTGGTGCAACTCA
CCGAGCAAGGTTACTTTGCCTTTGCTAAGCAGGCTCTAGAAGAGCGTCGTCATGCCCTGATGCCGCCACTTAGTCATCTG
GTGTTACTGCGCGCAGAATCAGTCAAGCCAGAGCAAGGGCATTTATTTCTTGAGCAGGCCCATGCTTTAGCTGAGCAACT
GCAGCTGAGCTTAGACTGCCCTGAAGTTGAACTGCTAGGCCCAGTACCTGCCCCCATGGAGCGCCGCCAAGGCCGCCATC
GCGCACAGTTGCTACTGCTAAGTACCCAGCGCACCCAATTGCACTGTTTAGTGAACGCCTTAGTGCATAATTTAGAGCCT
TTACCCAGTGCCCGAAGCGTACGCTGGTCTGTTGATGTTGACCCAATCGAGCTGTTTTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(201-364)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.715

100

0.48

Multiple sequence alignment