Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACEN2W_RS23440 Genome accession   NZ_CP168246
Coordinates   3125224..3126723 (+) Length   499 a.a.
NCBI ID   WP_346824836.1    Uniprot ID   -
Organism   Ralstonia solanacearum strain 071-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3120224..3131723
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN2W_RS23415 (ACEN2W_23405) - 3121403..3122386 (+) 984 WP_014618022.1 SIS domain-containing protein -
  ACEN2W_RS23420 (ACEN2W_23410) - 3122419..3123006 (+) 588 WP_373338328.1 KdsC family phosphatase -
  ACEN2W_RS23425 (ACEN2W_23415) lptC 3123008..3123640 (+) 633 WP_014618024.1 LPS export ABC transporter periplasmic protein LptC -
  ACEN2W_RS23430 (ACEN2W_23420) lptA 3123708..3124304 (+) 597 WP_346836308.1 lipopolysaccharide transport periplasmic protein LptA -
  ACEN2W_RS23435 (ACEN2W_23425) lptB 3124338..3125126 (+) 789 WP_346836309.1 LPS export ABC transporter ATP-binding protein -
  ACEN2W_RS23440 (ACEN2W_23430) rpoN/rpoN1 3125224..3126723 (+) 1500 WP_346824836.1 RNA polymerase factor sigma-54 Machinery gene
  ACEN2W_RS23445 (ACEN2W_23435) hpf 3126775..3127128 (+) 354 WP_003262275.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACEN2W_RS23450 (ACEN2W_23440) ptsN 3127478..3127933 (+) 456 WP_003262274.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACEN2W_RS23455 (ACEN2W_23445) hprK 3128004..3128978 (+) 975 WP_346824837.1 HPr(Ser) kinase/phosphatase -
  ACEN2W_RS23460 (ACEN2W_23450) - 3129169..3129501 (+) 333 WP_039550978.1 PsiF family protein -
  ACEN2W_RS23465 (ACEN2W_23455) rapZ 3129595..3130485 (+) 891 WP_014618030.1 RNase adapter RapZ -
  ACEN2W_RS23470 (ACEN2W_23460) - 3130563..3131216 (+) 654 WP_014618031.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55157.57 Da        Isoelectric Point: 4.9028

>NTDB_id=1043445 ACEN2W_RS23440 WP_346824836.1 3125224..3126723(+) (rpoN/rpoN1) [Ralstonia solanacearum strain 071-2]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVSALQSNSATPPPTE
SPPPAANGSDHADTAEASYENGSDFDSDYGSDRSDWSLDDFARKPQSDEDNRAPMQLREAEPTLREFLMEQLAPLKLSMR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLESPAKPLAL
AIVNQHLELLAARDYTRLKKALSVDEGALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=1043445 ACEN2W_RS23440 WP_346824836.1 3125224..3126723(+) (rpoN/rpoN1) [Ralstonia solanacearum strain 071-2]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCTCAGCATCTGGCGCTCACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAACTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTGGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGATGGTTCGGTCAGCGCGCTGCAGAGCAACAGCGCCACCCCGCCGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGCTCGGACCACGCCGACACAGCCGAAGCATCGTACGAAAACGGCAGCGACTTCGACAG
CGACTACGGCTCCGATCGCTCCGACTGGAGCCTCGACGACTTCGCACGCAAACCCCAGTCCGACGAAGACAACCGCGCCC
CGATGCAGCTGCGCGAGGCCGAGCCCACGCTGCGCGAATTCCTGATGGAACAGCTGGCGCCGCTCAAATTGTCCATGCGT
GACAAGGGCCTCGTCATCTTCCTGATCGAGTCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGACGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGTCGAACTCGACGAACTGCAAGCCGCGCTGCGCATGCTGCAGAGCTTCGACCCGGCCG
GCATCGGTGCACGCTCGGCTGCGGAATGCCTGACGCTGCAGTTGCAGCGCCTGGAGTCGCCAGCCAAGCCGCTCGCGCTG
GCCATCGTCAACCAGCATCTCGAGCTGCTCGCCGCGCGCGATTACACGCGCCTGAAGAAAGCGCTGTCCGTCGACGAAGG
CGCGCTGAAGGCCGCGCATGAACTGATCCGCTCGCTGGCGCCGTTCCCGGGCCACGCCTTCGGGCGCGCCGAGGCCGACT
TCGTCGTGCCCGATGTCGTGGTGCGTAAAACCGCCGCAGGCTGGATGGCGCAACTGAACCCCGACGTGATGCCGCGCCTG
CGCATCAACGACATGTACGCGCAGATCCTGCGCAGCAGCCGCGGCGAGTCGGGCGCCGCGAACCTGCAGCAAAAGCTGCA
GGAAGCGCGCTGGCTGATCAAAAACATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCGATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAGTACATGGCGACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAAATCCCAGCAGTGAATTTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

97.395

100

0.974

Multiple sequence alignment