Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACEN3H_RS15860 Genome accession   NZ_CP168231
Coordinates   3398722..3399522 (-) Length   266 a.a.
NCBI ID   WP_201783225.1    Uniprot ID   -
Organism   Acinetobacter lactucae strain 2024CK-01095     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3393722..3404522
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN3H_RS15840 (ACEN3H_15840) - 3394787..3395485 (-) 699 WP_061874264.1 tetratricopeptide repeat protein -
  ACEN3H_RS15845 (ACEN3H_15845) hisS 3395514..3396806 (-) 1293 WP_373358069.1 histidine--tRNA ligase -
  ACEN3H_RS15850 (ACEN3H_15850) ispG 3396803..3397918 (-) 1116 WP_016144301.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACEN3H_RS15855 (ACEN3H_15855) - 3397940..3398731 (-) 792 WP_373358070.1 helix-turn-helix domain-containing protein -
  ACEN3H_RS15860 (ACEN3H_15860) pilF 3398722..3399522 (-) 801 WP_201783225.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACEN3H_RS15865 (ACEN3H_15865) rlmN 3399537..3400772 (-) 1236 WP_373358071.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACEN3H_RS15870 (ACEN3H_15870) ndk 3400896..3401327 (-) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  ACEN3H_RS15875 (ACEN3H_15875) iscX 3401441..3401641 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  ACEN3H_RS15880 (ACEN3H_15880) - 3401741..3402292 (-) 552 WP_016144298.1 phosphatase PAP2 family protein -
  ACEN3H_RS15885 (ACEN3H_15885) - 3402317..3403603 (-) 1287 WP_087090003.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30046.30 Da        Isoelectric Point: 9.2541

>NTDB_id=1043326 ACEN3H_RS15860 WP_201783225.1 3398722..3399522(-) (pilF) [Acinetobacter lactucae strain 2024CK-01095]
MSIPKLKTVLCMGIAVAFLASGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKTNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMGRYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
VNAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQALVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1043326 ACEN3H_RS15860 WP_201783225.1 3398722..3399522(-) (pilF) [Acinetobacter lactucae strain 2024CK-01095]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCAGTTGCATTTCTGGCAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCGGAAAAAGCAGTAAAAGTGCGTACACAACTTGCTGCTGAACATATCCGTTCTGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCAACAGCAAACATGATGATGGGGATTTTG
CTACAGCAAGAAGGTAGCAAAACCAACTTAGAAAAAGCAGAACATTATTTTAAACGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATACCAAATGGGGCGTTATAATGATGCAATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GTGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGTGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCTCTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGCGTTAGTAAACCAG
TTACGTGCCTTGTTCCCAGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_16)

(89-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.609

100

0.936
  pilF Acinetobacter baylyi ADP1

75.385

97.744

0.737

Multiple sequence alignment