Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACEN3N_RS18515 Genome accession   NZ_CP168228
Coordinates   3935864..3936667 (-) Length   267 a.a.
NCBI ID   WP_373350541.1    Uniprot ID   -
Organism   Acinetobacter bereziniae strain 2024CK-01176     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3930864..3941667
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN3N_RS18495 (ACEN3N_18495) - 3931924..3932625 (-) 702 WP_004825005.1 tetratricopeptide repeat protein -
  ACEN3N_RS18500 (ACEN3N_18500) hisS 3932656..3933951 (-) 1296 WP_373350540.1 histidine--tRNA ligase -
  ACEN3N_RS18505 (ACEN3N_18505) ispG 3933948..3935063 (-) 1116 WP_004825009.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACEN3N_RS18510 (ACEN3N_18510) - 3935085..3935873 (-) 789 WP_004825011.1 RodZ domain-containing protein -
  ACEN3N_RS18515 (ACEN3N_18515) pilF 3935864..3936667 (-) 804 WP_373350541.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACEN3N_RS18520 (ACEN3N_18520) rlmN 3936728..3937978 (-) 1251 WP_042090323.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACEN3N_RS18525 (ACEN3N_18525) ndk 3938155..3938586 (-) 432 WP_004725878.1 nucleoside-diphosphate kinase -
  ACEN3N_RS18530 (ACEN3N_18530) iscX 3938729..3938929 (-) 201 WP_004725877.1 Fe-S cluster assembly protein IscX -
  ACEN3N_RS18535 (ACEN3N_18535) - 3939103..3939654 (-) 552 WP_004825017.1 phosphatase PAP2 family protein -
  ACEN3N_RS18540 (ACEN3N_18540) - 3939685..3940971 (-) 1287 WP_373350542.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 29961.07 Da        Isoelectric Point: 9.2123

>NTDB_id=1043280 ACEN3N_RS18515 WP_373350541.1 3935864..3936667(-) (pilF) [Acinetobacter bereziniae strain 2024CK-01176]
MYQKAVVILAIGLSALVVQGCQTTNSDIIIKKDPEKAVKVRTQLAAEYIRSGDLDAAKRALDQALETDSRDSAANMMMGV
LLQQEGSKISMEKADGYFKRAISSDSKNAQARNNYGTYLFVSERYNDAIEQLSIAGASLGYDQRYKALENLGRVYLKVGD
TANAEKAFKNALQVNRDSIISLVELSEIFYLQQKNAQASQLYEQYVRLVGQDKQGARALWIGLRIARANNDQMGMQVLVN
QLRALFPDSKEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=1043280 ACEN3N_RS18515 WP_373350541.1 3935864..3936667(-) (pilF) [Acinetobacter bereziniae strain 2024CK-01176]
ATGTATCAAAAAGCAGTTGTAATATTGGCAATTGGTTTATCTGCATTGGTGGTACAGGGTTGTCAAACGACAAACTCAGA
CATAATTATAAAAAAAGATCCTGAGAAAGCCGTAAAAGTTCGTACCCAGTTAGCTGCGGAGTATATTCGATCAGGCGATC
TGGATGCAGCCAAACGTGCACTTGATCAAGCCCTTGAAACAGATTCGCGAGATTCAGCTGCCAATATGATGATGGGGGTT
TTACTACAACAAGAAGGTAGTAAGATCAGCATGGAAAAAGCGGATGGCTATTTTAAGCGTGCGATTTCCAGTGATTCGAA
AAATGCGCAGGCTCGTAATAATTATGGAACATATTTATTTGTTAGCGAGCGTTATAATGATGCGATTGAGCAGTTGAGTA
TCGCAGGCGCATCTCTTGGATATGACCAGCGTTATAAGGCTTTAGAAAATTTGGGGCGGGTATATTTAAAAGTTGGTGAT
ACAGCAAATGCCGAAAAAGCATTCAAAAATGCCTTACAAGTCAATCGCGATTCGATCATTTCTTTGGTAGAGTTGTCTGA
GATTTTCTATTTGCAACAAAAAAATGCGCAAGCAAGTCAACTCTATGAACAATATGTTCGTTTAGTAGGTCAGGACAAAC
AGGGCGCAAGAGCACTCTGGATTGGTTTACGTATTGCACGTGCCAATAATGACCAGATGGGAATGCAAGTACTGGTGAAC
CAGTTGCGGGCATTATTCCCTGACAGTAAAGAATATCAACGTTATCTGCAATTACAGTACAGTACTGAGGCCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(116-173)

Tetratricopeptide repeat (TPR_16)

(44-79)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

74.717

99.251

0.742
  pilF Acinetobacter baylyi ADP1

67.843

95.506

0.648

Multiple sequence alignment