Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACEF29_RS09030 Genome accession   NZ_CP168150
Coordinates   1845901..1846335 (+) Length   144 a.a.
NCBI ID   WP_053573457.1    Uniprot ID   -
Organism   Bacillus velezensis strain CM19     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1840901..1851335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEF29_RS09025 (ACEF29_09025) - 1844401..1845612 (-) 1212 WP_039254928.1 cytochrome P450 -
  ACEF29_RS09030 (ACEF29_09030) nucA/comI 1845901..1846335 (+) 435 WP_053573457.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACEF29_RS09035 (ACEF29_09035) - 1846395..1847150 (+) 756 WP_003154084.1 YoaK family protein -
  ACEF29_RS09040 (ACEF29_09040) - 1847179..1847541 (-) 363 WP_053573458.1 hypothetical protein -
  ACEF29_RS09045 (ACEF29_09045) - 1847734..1849062 (-) 1329 WP_015417518.1 S8 family peptidase -
  ACEF29_RS09050 (ACEF29_09050) - 1849241..1849474 (+) 234 WP_015239897.1 hypothetical protein -
  ACEF29_RS09055 (ACEF29_09055) - 1849730..1850437 (+) 708 WP_007410381.1 poly-gamma-glutamate hydrolase family protein -
  ACEF29_RS09060 (ACEF29_09060) - 1850497..1850949 (+) 453 WP_014305039.1 OsmC family protein -
  ACEF29_RS09065 (ACEF29_09065) - 1850963..1851316 (-) 354 WP_007410379.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15548.46 Da        Isoelectric Point: 7.2418

>NTDB_id=1042615 ACEF29_RS09030 WP_053573457.1 1845901..1846335(+) (nucA/comI) [Bacillus velezensis strain CM19]
MNAFMKWAASLLLVISLQFGFTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADVRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1042615 ACEF29_RS09030 WP_053573457.1 1845901..1846335(+) (nucA/comI) [Bacillus velezensis strain CM19]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCTTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCAGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATGTCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

59.829

81.25

0.486

Multiple sequence alignment