Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACDZ78_RS01800 Genome accession   NZ_CP168026
Coordinates   370197..370634 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain EM5 isolate     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 365197..375634
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDZ78_RS01775 (ACDZ78_01775) - 366216..367352 (+) 1137 WP_025284207.1 zinc-dependent alcohol dehydrogenase -
  ACDZ78_RS01780 (ACDZ78_01780) - 367367..367801 (+) 435 WP_012116767.1 RDD family protein -
  ACDZ78_RS01785 (ACDZ78_01785) - 367919..368197 (+) 279 WP_263612330.1 YckD family protein -
  ACDZ78_RS01790 (ACDZ78_01790) - 368301..369737 (+) 1437 WP_053573745.1 glycoside hydrolase family 1 protein -
  ACDZ78_RS01795 (ACDZ78_01795) nin/comJ 369778..370176 (-) 399 WP_020955304.1 competence protein ComJ Regulator
  ACDZ78_RS01800 (ACDZ78_01800) nucA/comI 370197..370634 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACDZ78_RS01805 (ACDZ78_01805) hxlB 370993..371550 (-) 558 WP_053573744.1 6-phospho-3-hexuloisomerase -
  ACDZ78_RS01810 (ACDZ78_01810) hxlA 371547..372182 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  ACDZ78_RS01815 (ACDZ78_01815) - 372414..372776 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=1041443 ACDZ78_RS01800 WP_003156588.1 370197..370634(-) (nucA/comI) [Bacillus velezensis strain EM5 isolate]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1041443 ACDZ78_RS01800 WP_003156588.1 370197..370634(-) (nucA/comI) [Bacillus velezensis strain EM5 isolate]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGAGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment