Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABQ091_RS07375 Genome accession   NZ_CP167993
Coordinates   1706312..1707442 (+) Length   376 a.a.
NCBI ID   WP_005990431.1    Uniprot ID   Q8P759
Organism   Xanthomonas vesicatoria strain WHRI 8302     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1701312..1712442
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABQ091_RS07350 (ABQ091_007350) - 1701548..1702129 (-) 582 WP_005990427.1 M23 family metallopeptidase -
  ABQ091_RS07355 (ABQ091_007355) - 1702281..1702703 (-) 423 WP_005990428.1 HU family DNA-binding protein -
  ABQ091_RS07360 (ABQ091_007360) proC 1703230..1704084 (-) 855 WP_005990429.1 pyrroline-5-carboxylate reductase -
  ABQ091_RS07365 (ABQ091_007365) - 1704133..1704819 (-) 687 WP_039420053.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABQ091_RS07370 (ABQ091_007370) - 1705145..1706183 (+) 1039 Protein_1436 type IV pilus twitching motility protein PilT -
  ABQ091_RS07375 (ABQ091_007375) pilU 1706312..1707442 (+) 1131 WP_005990431.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABQ091_RS07380 (ABQ091_007380) - 1707706..1708380 (+) 675 WP_042827786.1 YitT family protein -
  ABQ091_RS07385 (ABQ091_007385) - 1708386..1709285 (-) 900 WP_058563161.1 DUF72 domain-containing protein -
  ABQ091_RS07390 (ABQ091_007390) - 1709496..1710725 (-) 1230 WP_058563160.1 hypothetical protein -
  ABQ091_RS07395 (ABQ091_007395) - 1710722..1711261 (-) 540 WP_058563159.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41996.26 Da        Isoelectric Point: 6.8287

>NTDB_id=1040964 ABQ091_RS07375 WP_005990431.1 1706312..1707442(+) (pilU) [Xanthomonas vesicatoria strain WHRI 8302]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1040964 ABQ091_RS07375 WP_005990431.1 1706312..1707442(+) (pilU) [Xanthomonas vesicatoria strain WHRI 8302]
ATGAGCACCATCGACTTCACTTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTTTTCATCACCTCCGGCAT
GCCGCCGGCGATCAAGGTGCATGGCAAGATCAGCCCGATCACGCAGACGCCGTTGACCGCGCAGCAGAGCCGCGACCTGG
TGTTGAACGTGATGACGCCATCGCAGCGCGAGGAGTTCGAAAAGACCCACGAATGCAATTTCGCCATCGGCGTGTCCGGG
GTCGGGCGCTTCCGTGTGAGCTGTTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCGCATTCC
CACCGTGGAAGAGCTGAGCCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGTAAGTCCACCTCGCTGGCGGCGATGATCGGCTATCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGATACCGACAG
TTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGATCACGCGATCGCCTTCGCTGAAACCGGCCACCTGGTGCTGTGCACGCTGCATGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTTCCGGAAGATCGCCGCAACCAGCTGCTGATGGACCTGTCGCTGAACCTCAAGGGCGTGGT
GGCGCAGCAGCTGATCCCGACCCCCGATGGCCGCGGCCGCCGCGTGGCGATGGAAATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCTTGTTCGAGTTGTATCAGGCCGGCGAAATCAGTTACGAAGACGCGCTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTACGCATCAAGCTTTCGCAAGGCGGCGATGCCAAGACCTTGTCGCAGGGGCTGGATGGCGTGGAGATCGCCG
AGGTTCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8P759

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367

Multiple sequence alignment