Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACDW34_RS01400 Genome accession   NZ_CP167896
Coordinates   299505..300563 (+) Length   352 a.a.
NCBI ID   WP_130074425.1    Uniprot ID   -
Organism   Acinetobacter piscicola strain p38     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 294505..305563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDW34_RS01390 (ACDW34_01390) - 294737..296326 (+) 1590 WP_174493487.1 TerC family protein -
  ACDW34_RS01395 (ACDW34_01395) ponA 296770..299343 (-) 2574 WP_372403867.1 penicillin-binding protein PBP1a -
  ACDW34_RS01400 (ACDW34_01400) pilM 299505..300563 (+) 1059 WP_130074425.1 pilus assembly protein PilM Machinery gene
  ACDW34_RS01405 (ACDW34_01405) pilN 300563..301195 (+) 633 WP_130074426.1 PilN domain-containing protein Machinery gene
  ACDW34_RS01410 (ACDW34_01410) - 301192..301855 (+) 664 Protein_271 type 4a pilus biogenesis protein PilO -
  ACDW34_RS01415 (ACDW34_01415) pilP 302001..302528 (+) 528 WP_130074428.1 pilus assembly protein PilP Machinery gene
  ACDW34_RS01420 (ACDW34_01420) pilQ 302545..304710 (+) 2166 WP_372403868.1 type IV pilus secretin PilQ Machinery gene
  ACDW34_RS01425 (ACDW34_01425) aroK 304747..305289 (+) 543 WP_130074430.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38999.65 Da        Isoelectric Point: 4.4486

>NTDB_id=1040149 ACDW34_RS01400 WP_130074425.1 299505..300563(+) (pilM) [Acinetobacter piscicola strain p38]
MLRISRKQVKGLIGVDISSTSVKILELSVKNGRYWVESYGLSPLLDGSVVEKNILNPEAVSDALERAMNIANPQSTNAAV
AVTNSMVIQKVIEMDADMNDEEREVQIRMDAEQYIPFPLDEVSLDFEVLPDKLPNPNRVNVLLVATRTENVDSRVEVLEI
TGLTAKVVDTESYCMERAFEVFADTLPIGANMVGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTQDVQARYGLSYEE
AGRAKKDRSLPDDYETEVLIPFLEAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTVANPFL
QMGFSPQIDLKKIENDAPSLMVACGLAMRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=1040149 ACDW34_RS01400 WP_130074425.1 299505..300563(+) (pilM) [Acinetobacter piscicola strain p38]
GTGCTTAGAATAAGTCGTAAGCAAGTTAAGGGGCTTATAGGGGTAGATATCAGTTCAACCTCTGTAAAGATATTAGAACT
CTCTGTTAAAAACGGTCGTTATTGGGTGGAAAGCTATGGATTAAGTCCATTGTTAGATGGAAGTGTGGTTGAGAAGAATA
TTCTCAACCCAGAAGCAGTTTCGGATGCTTTAGAGCGTGCGATGAATATTGCCAATCCTCAATCCACGAATGCGGCTGTA
GCAGTGACTAACTCTATGGTGATTCAAAAAGTCATTGAAATGGATGCAGACATGAACGATGAAGAACGCGAAGTTCAAAT
TCGTATGGATGCAGAACAATATATTCCATTCCCATTAGATGAAGTCAGTCTAGACTTTGAGGTTTTACCTGACAAGTTAC
CTAACCCAAATCGGGTGAATGTATTGCTTGTTGCAACACGTACTGAAAATGTCGACTCGCGCGTTGAAGTACTTGAGATT
ACTGGATTGACTGCAAAAGTGGTTGATACAGAGAGCTATTGCATGGAGCGTGCCTTTGAAGTATTCGCGGATACTTTACC
TATTGGCGCGAATATGGTCGGGATCTTAGATATCGGGCATACCATGACTACACTGTCTGTCATGCAAAACGGTAAAATTA
TCTATACGCGTGAACAAGTCTTTGGGGGGAAACAGCTGACCCAAGATGTACAAGCGCGTTATGGTCTTTCTTATGAAGAA
GCGGGCCGTGCCAAAAAAGATCGCTCATTGCCTGATGACTATGAAACAGAAGTGTTGATTCCATTTTTGGAAGCTGTTGT
TCAACAAGCAGCACGCTCATTACAATTCTTTTTCTCATCCTCTCAATTTAATGAAATTGATCATATTTTATTGGCTGGAG
GCAATGCCAATATTCCAGGACTTGCGAAATTATTACAACAAAAATTGGGTTATCGAGTCACTGTGGCAAATCCTTTCTTA
CAAATGGGTTTTTCACCACAAATCGATTTGAAAAAAATAGAAAATGATGCACCTTCCTTAATGGTCGCATGTGGCTTAGC
AATGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

82.955

100

0.83
  comM Acinetobacter nosocomialis M2

82.955

100

0.83
  comM Acinetobacter baylyi ADP1

79.261

100

0.793
  pilM Legionella pneumophila strain ERS1305867

41.69

100

0.42

Multiple sequence alignment