Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QUE65_RS09690 Genome accession   NZ_AP028139
Coordinates   1861338..1862243 (+) Length   301 a.a.
NCBI ID   WP_286280904.1    Uniprot ID   -
Organism   Polynucleobacter sp. HIN8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1856338..1867243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE65_RS09660 (PHIN8_18790) pth 1856512..1857102 (+) 591 WP_286280897.1 aminoacyl-tRNA hydrolase -
  QUE65_RS09665 (PHIN8_18800) - 1857108..1857368 (-) 261 WP_286280898.1 YfhL family 4Fe-4S dicluster ferredoxin -
  QUE65_RS09670 (PHIN8_18810) coaD 1857401..1857892 (-) 492 WP_215370392.1 pantetheine-phosphate adenylyltransferase -
  QUE65_RS09675 (PHIN8_18820) rsmD 1857927..1858517 (-) 591 WP_286280899.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  QUE65_RS09680 (PHIN8_18830) - 1858518..1859858 (-) 1341 WP_286280900.1 pitrilysin family protein -
  QUE65_RS09685 (PHIN8_18840) - 1859870..1861288 (-) 1419 WP_286280901.1 pitrilysin family protein -
  QUE65_RS09690 (PHIN8_18850) pilA 1861338..1862243 (+) 906 WP_286280904.1 signal recognition particle-docking protein FtsY Machinery gene
  QUE65_RS09695 (PHIN8_18860) rpoH 1862377..1863267 (+) 891 WP_286280906.1 RNA polymerase sigma factor RpoH -
  QUE65_RS09700 (PHIN8_18870) - 1863271..1863843 (-) 573 WP_286280908.1 SCO family protein -
  QUE65_RS09705 (PHIN8_18880) cyoE 1863847..1864704 (-) 858 WP_215372703.1 heme o synthase -
  QUE65_RS09710 (PHIN8_18890) - 1864731..1865855 (-) 1125 WP_286280910.1 COX15/CtaA family protein -
  QUE65_RS09715 (PHIN8_18900) - 1865852..1866505 (-) 654 WP_286280911.1 hypothetical protein -

Sequence

Protein


Download         Length: 301 a.a.        Molecular weight: 32541.14 Da        Isoelectric Point: 6.8871

>NTDB_id=104012 QUE65_RS09690 WP_286280904.1 1861338..1862243(+) (pilA) [Polynucleobacter sp. HIN8]
MFGLRKTLSSLFTRHRVDEAWFDHLEELLIKADVGVATSTFLITSLRKSAKEHAITNSEDLKADLVSELSHHLSDLESPE
NPLNPSAIHAKPEVWLIVGVNGAGKTTSIGKLCHHFQAQGKSVLLAAGDTFRAAARNQLKEWGERNQVQVLSQESGDAGA
IAHDAIQAALSRQVDIVLIDTAGRLATQTNLMEELKKVKRVISKLIPDAPHQMVLVLDGNTGQNGIAQVLAFRNAIGLHG
LIVTKLDGTAKGGVICALRNELENAPKNQGPKTYVYALGKGEQLGDLEPFSAQAYAKELVE

Nucleotide


Download         Length: 906 bp        

>NTDB_id=104012 QUE65_RS09690 WP_286280904.1 1861338..1862243(+) (pilA) [Polynucleobacter sp. HIN8]
ATGTTTGGTTTGCGTAAAACACTTAGTTCACTTTTTACTCGTCACCGTGTTGATGAGGCCTGGTTTGACCATTTAGAAGA
ATTACTAATTAAAGCCGATGTCGGGGTTGCGACATCGACATTCCTAATCACTAGTCTACGCAAATCTGCAAAAGAACATG
CCATTACAAATTCTGAGGATTTAAAAGCCGATCTTGTAAGTGAACTAAGCCATCACTTGAGCGACTTGGAGTCCCCTGAA
AACCCCCTAAATCCCTCTGCGATCCATGCAAAGCCTGAAGTTTGGCTGATCGTAGGGGTCAATGGTGCTGGCAAAACCAC
ATCCATTGGAAAACTATGCCACCACTTCCAAGCTCAAGGAAAATCGGTACTGCTTGCTGCCGGTGATACATTCCGAGCAG
CGGCTCGCAATCAACTCAAAGAATGGGGTGAGCGCAATCAAGTTCAAGTGCTTAGTCAAGAAAGCGGTGATGCGGGCGCT
ATTGCACATGATGCAATCCAGGCTGCGCTATCAAGGCAAGTGGATATCGTATTAATTGATACGGCTGGTCGACTGGCCAC
TCAGACTAACCTCATGGAAGAATTAAAGAAGGTTAAGCGAGTCATTTCGAAGTTGATTCCGGATGCCCCACATCAAATGG
TGTTGGTCCTTGATGGAAATACAGGACAAAATGGAATTGCCCAGGTTTTGGCCTTTCGGAACGCGATAGGACTGCACGGT
TTAATCGTTACTAAATTAGATGGCACCGCTAAGGGCGGGGTAATTTGTGCGCTTCGCAATGAATTAGAAAACGCCCCCAA
AAACCAGGGTCCTAAAACCTATGTCTACGCCCTTGGCAAGGGTGAGCAATTAGGTGATTTGGAGCCCTTTTCAGCCCAAG
CCTATGCTAAAGAGCTCGTAGAATAG

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(3-69)

SRP54-type protein, GTPase domain (SRP54)

(92-300)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.164

99.336

0.488

Multiple sequence alignment