Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACDH63_RS17060 Genome accession   NZ_CP167871
Coordinates   4021981..4023114 (+) Length   377 a.a.
NCBI ID   WP_008576907.1    Uniprot ID   Q3BP52
Organism   Xanthomonas axonopodis pv. maculifoliigardeniae strain NCPPB 971     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4016981..4028114
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDH63_RS17035 (ACDH63_17035) - 4017150..4018337 (-) 1188 WP_031424950.1 cystathionine gamma-synthase -
  ACDH63_RS17040 (ACDH63_17040) - 4018437..4019807 (-) 1371 WP_218540181.1 pyridoxal-phosphate dependent enzyme -
  ACDH63_RS17045 (ACDH63_17045) - 4020042..4020260 (-) 219 WP_003484103.1 YdcH family protein -
  ACDH63_RS17050 (ACDH63_17050) - 4020612..4021502 (-) 891 WP_031424951.1 membrane protein -
  ACDH63_RS17055 (ACDH63_17055) - 4021499..4021882 (-) 384 WP_042774027.1 DUF4398 domain-containing protein -
  ACDH63_RS17060 (ACDH63_17060) pilU 4021981..4023114 (+) 1134 WP_008576907.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACDH63_RS17065 (ACDH63_17065) maiA 4023446..4024108 (-) 663 WP_109291097.1 maleylacetoacetate isomerase -
  ACDH63_RS17070 (ACDH63_17070) - 4024140..4025198 (-) 1059 WP_181388266.1 fumarylacetoacetate hydrolase family protein -
  ACDH63_RS17075 (ACDH63_17075) - 4025507..4026895 (+) 1389 WP_008576904.1 DEAD/DEAH box helicase -
  ACDH63_RS17080 (ACDH63_17080) queD 4027031..4027384 (+) 354 WP_008576903.1 6-carboxytetrahydropterin synthase QueD -
  ACDH63_RS17085 (ACDH63_17085) - 4027576..4027911 (+) 336 WP_014091104.1 phasin family protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41692.04 Da        Isoelectric Point: 6.1401

>NTDB_id=1040066 ACDH63_RS17060 WP_008576907.1 4021981..4023114(+) (pilU) [Xanthomonas axonopodis pv. maculifoliigardeniae strain NCPPB 971]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGVDGRRRPATEVLLNTPMIRDLLRRGQVHEVKAAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYEAAAAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1040066 ACDH63_RS17060 WP_008576907.1 4021981..4023114(+) (pilU) [Xanthomonas axonopodis pv. maculifoliigardeniae strain NCPPB 971]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAGGGCAAGCTTTACCCGCTCGGCAATACGGGGTTGCCGCCGGGAATGGTCAAGAAAATCGCCTACTCGT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAACGCGAGCTCGAGCTCAACATGGCCATCGCCCTGCCCGATGCCGGCCGT
TTCCGCGTGAACGTTTTCAAGCAGCGCGGCGAGGTGGGCATGGTGATTCGCGCCATTCGCAGCCGGATTCCCAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGTTCGACCGGTT
CGGGCAAGTCGACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACGCACGCCTTCCAGAG
CGCGCTGAAGAACGCCATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCCGAAACCATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAATTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAATCTGCGCGCAGTGGTCTCCCAGCG
CCTGGTCAAGGGCGTGGACGGGCGCCGCCGCCCCGCCACCGAGGTGTTGCTCAATACCCCGATGATCCGCGACCTGCTAC
GGCGCGGCCAGGTGCACGAGGTCAAGGCAGCGATGGAAGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAATGCCTG
TTCCGGATGGTGAAGTCGGGCGAAATCGAGCAGGAAGAAGCACTGCGCGCGGCCGACTCGCGCGACGGCCTGGCGCTGAA
GTTCCGTCTCTCCGAGGGCGGCTCCGGCGAGCACGACCCGTATGCGGATTACGAAGCGGCCGCGGCACCGAAGATCAGTC
ACGGGTTCATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3BP52

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.368

95.225

0.499
  pilU Vibrio cholerae strain A1552

49.444

95.491

0.472
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.241

100

0.448
  pilT Pseudomonas aeruginosa PAK

42.899

89.655

0.385
  pilT Acinetobacter baumannii D1279779

41.42

89.655

0.371
  pilT Acinetobacter nosocomialis M2

41.42

89.655

0.371
  pilT Acinetobacter baumannii strain A118

41.42

89.655

0.371
  pilT Legionella pneumophila strain Lp02

41.42

89.655

0.371
  pilT Legionella pneumophila strain ERS1305867

41.42

89.655

0.371
  pilT Pseudomonas stutzeri DSM 10701

41.617

88.594

0.369
  pilT Acinetobacter baylyi ADP1

40.882

90.186

0.369
  pilT Vibrio cholerae strain A1552

41.141

88.329

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.141

88.329

0.363
  pilT Neisseria gonorrhoeae MS11

39.193

92.042

0.361
  pilT Neisseria meningitidis 8013

39.193

92.042

0.361

Multiple sequence alignment