Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   QUE65_RS03135 Genome accession   NZ_AP028139
Coordinates   586444..587805 (+) Length   453 a.a.
NCBI ID   WP_286281837.1    Uniprot ID   -
Organism   Polynucleobacter sp. HIN8     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 581444..592805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE65_RS03105 (PHIN8_06010) - 581601..581780 (+) 180 WP_286281831.1 hypothetical protein -
  QUE65_RS03110 (PHIN8_06020) - 582578..583543 (+) 966 WP_286281832.1 hypothetical protein -
  QUE65_RS03115 - 583592..583774 (+) 183 WP_286281833.1 hypothetical protein -
  QUE65_RS03125 (PHIN8_06030) - 584107..585948 (+) 1842 WP_286282414.1 potassium transporter Kup -
  QUE65_RS03130 (PHIN8_06040) - 585948..586442 (+) 495 WP_286281835.1 hypothetical protein -
  QUE65_RS03135 (PHIN8_06050) radA 586444..587805 (+) 1362 WP_286281837.1 DNA repair protein RadA Machinery gene
  QUE65_RS03140 (PHIN8_06060) prmB 587812..588681 (-) 870 WP_286282416.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  QUE65_RS03145 (PHIN8_06070) dapE 588743..589891 (-) 1149 WP_286281839.1 succinyl-diaminopimelate desuccinylase -
  QUE65_RS03150 (PHIN8_06080) dapD 589895..590722 (-) 828 WP_286281841.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 48272.01 Da        Isoelectric Point: 7.1451

>NTDB_id=104003 QUE65_RS03135 WP_286281837.1 586444..587805(+) (radA) [Polynucleobacter sp. HIN8]
MAKTKTLYVCQACGGSSPKWQGQCPACDAWNTLEESLAETANHRFQGLAKSIPRQRLVSIEAQDSPRLPTGIVELDRVLG
GGLVAGGVVLLGGDPGIGKSTLLLQALAELSMQGVDVLYSSGEESAAQIALRAQRISLNAPQLEILAEIQLEKLLLSVEA
AQPQVLVVDSIQTLYSEAFTSAPGSVAQVRECAAQLTRLAKSSGICILLVGHVTKDGHLAGPRVLEHIVDTVLYFEGDTH
SSFRLVRSIKNRFGAVNELGVFAMTEKGLKGVNNPSALFLSQHPQTVPGSCVLVTQEGSRPLLVEIQALVDTAHIPNPRR
LAVGLEQARLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLAITSSIRNRALPKQLVVFGEVGLAGEIRP
CPRGQERLKEAAKLGFKIAIIPKANLPKSKMTDLQMIAVERIDEAIAAANDLF

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=104003 QUE65_RS03135 WP_286281837.1 586444..587805(+) (radA) [Polynucleobacter sp. HIN8]
ATGGCAAAGACCAAAACACTTTACGTTTGCCAGGCCTGTGGGGGCAGTTCTCCCAAATGGCAGGGACAATGCCCTGCATG
CGACGCATGGAACACGCTTGAAGAGTCTCTCGCCGAGACTGCAAACCATCGTTTTCAAGGGCTTGCCAAGTCAATCCCAC
GACAAAGATTGGTGAGCATTGAGGCACAGGATTCGCCACGATTACCAACCGGAATTGTTGAACTCGATCGCGTCTTAGGC
GGAGGATTGGTGGCAGGTGGTGTGGTCTTACTCGGCGGTGATCCAGGTATTGGGAAGTCCACCTTATTATTGCAAGCCTT
AGCCGAGCTAAGTATGCAAGGGGTCGATGTTTTATATAGCTCCGGAGAGGAGTCTGCTGCTCAAATTGCATTGCGTGCTC
AGCGAATTTCCCTTAATGCACCTCAACTTGAAATCTTGGCAGAGATTCAGCTCGAAAAGTTATTGCTCTCGGTTGAGGCG
GCGCAACCGCAGGTTTTAGTGGTTGACTCGATTCAAACGCTCTATTCCGAGGCATTTACATCGGCGCCTGGATCGGTTGC
TCAAGTTCGTGAGTGCGCCGCCCAATTAACGCGGCTCGCGAAATCAAGTGGGATCTGTATTTTATTGGTTGGCCACGTTA
CGAAAGATGGTCATCTGGCTGGTCCTCGTGTGCTGGAGCATATTGTGGATACCGTTTTGTATTTTGAGGGCGATACGCAT
TCGTCATTTCGCTTAGTGCGCTCCATTAAAAACCGCTTTGGCGCAGTGAACGAGCTTGGCGTCTTTGCGATGACCGAGAA
AGGTCTTAAAGGGGTTAACAATCCCTCCGCACTTTTTCTGTCGCAACACCCGCAAACGGTGCCTGGTTCCTGTGTACTGG
TCACCCAAGAGGGTAGTCGACCGCTGTTGGTTGAGATTCAGGCGCTGGTTGATACGGCGCATATCCCCAATCCAAGGCGC
TTGGCGGTGGGTTTAGAGCAGGCACGCTTGGCTATGCTGTTAGCGGTTTTGCACCGTCACGCAGGCATCGCTTGTTTTGA
TCAAGATGTCTTTCTCAATGCCGTGGGTGGGGTCAAAATTACCGAGCCCGCCGCTGATCTTGCAGTTCTACTGGCCATCA
CATCCTCAATCCGCAATCGGGCTCTACCAAAACAGTTGGTGGTATTTGGTGAAGTTGGTTTGGCCGGAGAAATTCGTCCA
TGCCCTCGGGGTCAGGAACGACTCAAGGAAGCTGCTAAATTAGGATTTAAGATCGCCATTATTCCTAAGGCTAATTTGCC
AAAATCAAAAATGACTGATTTACAAATGATTGCTGTTGAGCGGATTGATGAGGCAATCGCTGCGGCGAACGACCTTTTCT
AA

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(358-449)

AAA domain (AAA_25)

(68-217)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.67

100

0.499
  radA Streptococcus mitis NCTC 12261

46.137

100

0.461
  radA Streptococcus pneumoniae D39

46.102

99.117

0.457
  radA Streptococcus pneumoniae TIGR4

46.102

99.117

0.457
  radA Streptococcus mitis SK321

46.102

99.117

0.457
  radA Streptococcus pneumoniae R6

46.102

99.117

0.457
  radA Streptococcus pneumoniae Rx1

46.102

99.117

0.457

Multiple sequence alignment