Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABRP16_RS07245 Genome accession   NZ_CP167825
Coordinates   1667102..1668229 (-) Length   375 a.a.
NCBI ID   WP_042613770.1    Uniprot ID   -
Organism   Stenotrophomonas lacuserhaii strain WHRI 8086     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1662102..1673229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABRP16_RS07230 (ABRP16_007230) - 1663329..1664729 (-) 1401 WP_182657198.1 DEAD/DEAH box helicase -
  ABRP16_RS07235 (ABRP16_007235) - 1665350..1666339 (+) 990 WP_349998866.1 fumarylacetoacetate hydrolase family protein -
  ABRP16_RS07240 (ABRP16_007240) maiA 1666336..1667022 (+) 687 WP_349998867.1 maleylacetoacetate isomerase -
  ABRP16_RS07245 (ABRP16_007245) pilU 1667102..1668229 (-) 1128 WP_042613770.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABRP16_RS07250 (ABRP16_007250) - 1668355..1668723 (+) 369 WP_319278141.1 DUF4398 domain-containing protein -
  ABRP16_RS07255 (ABRP16_007255) - 1668723..1669607 (+) 885 WP_349998868.1 hypothetical protein -
  ABRP16_RS07260 (ABRP16_007260) - 1669933..1670172 (+) 240 WP_349998869.1 YdcH family protein -
  ABRP16_RS07265 (ABRP16_007265) - 1670458..1671828 (+) 1371 WP_101100167.1 pyridoxal-phosphate dependent enzyme -
  ABRP16_RS07270 (ABRP16_007270) - 1671955..1673142 (+) 1188 WP_349998870.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41472.71 Da        Isoelectric Point: 6.1905

>NTDB_id=1039618 ABRP16_RS07245 WP_042613770.1 1667102..1668229(-) (pilU) [Stenotrophomonas lacuserhaii strain WHRI 8086]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLELNMAIALADTGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHRNVLMNLSLNLRGVISQRLVKGHDGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRMVKDGIVDIEEALRAADSRDGLALKFRLSEGSSGEHDPYADFSAGQPTISHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=1039618 ABRP16_RS07245 WP_042613770.1 1667102..1668229(-) (pilU) [Stenotrophomonas lacuserhaii strain WHRI 8086]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTTTTGACGACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTCTACCCGCTGGGCAACACCGGCCTGCCGCCGGGGATGGTCAAGAAAATCGCCTATTCGC
TGATGGACGAAGGCCAGGTGCCCCAGTTCGAACGCGACCTGGAGCTCAACATGGCGATCGCCCTGGCCGACACCGGGCGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAAGTCGGCATGGTCATCCGCGCCATCCGCAGCCGGATCCCCAGCATCGA
AGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACTCCGCGTGGCCTGGTGCTGGTGGTGGGGTCCACCGGCT
CGGGCAAATCGACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACGACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAAGTCGGCCTGGACACCCACGCCTTCCACAA
CGCACTGAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAAATCCTGGATGCGGAAACGATGGAGGCGG
CCATCGCCTTCGCCGAAACCGGGCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACGATCGAACGCATC
CTCAACTTCTTCCCGGAAAGCGCGCATCGCAACGTGCTGATGAACCTGTCGTTGAACCTGCGCGGGGTCATCAGCCAGCG
CCTGGTGAAGGGCCACGACGGCCGCCGCCTGCCGGCGACCGAAGTGCTGATCAACACCCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGGCGGCGATGGAAGCCTCGCTGGAAGAAGGCATGGAAAGCTTCGACCAGTGCCTG
TTCCGGATGGTCAAGGACGGCATCGTCGACATCGAAGAGGCGCTGCGCGCAGCCGATTCACGCGACGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAGGGCAGCAGCGGCGAGCACGATCCCTACGCGGACTTCTCCGCCGGCCAGCCCACCATCTCGCACG
GCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.975

95.733

0.488
  pilU Acinetobacter baylyi ADP1

49.02

95.2

0.467
  pilU Vibrio cholerae strain A1552

49.435

94.4

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.972

95.467

0.429
  pilT Pseudomonas aeruginosa PAK

42.604

90.133

0.384
  pilT Acinetobacter baumannii strain A118

41.124

90.133

0.371
  pilT Acinetobacter nosocomialis M2

41.124

90.133

0.371
  pilT Acinetobacter baumannii D1279779

41.124

90.133

0.371
  pilT Acinetobacter baylyi ADP1

40.882

90.667

0.371
  pilT Legionella pneumophila strain Lp02

40.828

90.133

0.368
  pilT Legionella pneumophila strain ERS1305867

40.828

90.133

0.368
  pilT Pseudomonas stutzeri DSM 10701

40.533

90.133

0.365
  pilT Vibrio cholerae strain A1552

40.841

88.8

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.8

0.363
  pilT Neisseria gonorrhoeae MS11

39.193

92.533

0.363
  pilT Neisseria meningitidis 8013

39.193

92.533

0.363

Multiple sequence alignment