Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACCO40_RS13030 Genome accession   NZ_CP167793
Coordinates   2548907..2549317 (-) Length   136 a.a.
NCBI ID   WP_014114486.1    Uniprot ID   -
Organism   Bacillus spizizenii strain SHT-15     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2543907..2554317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCO40_RS13000 (ACCO40_13000) - 2543923..2544063 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  ACCO40_RS13005 (ACCO40_13005) - 2544365..2545096 (-) 732 WP_061186847.1 SGNH/GDSL hydrolase family protein -
  ACCO40_RS13010 (ACCO40_13010) - 2545348..2546100 (-) 753 WP_114150361.1 N-acetylmuramoyl-L-alanine amidase -
  ACCO40_RS13015 (ACCO40_13015) - 2546295..2546921 (+) 627 WP_114150360.1 TVP38/TMEM64 family protein -
  ACCO40_RS13020 (ACCO40_13020) gnd 2546940..2547833 (-) 894 WP_079287983.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  ACCO40_RS13025 (ACCO40_13025) - 2548158..2548874 (+) 717 WP_372401706.1 DUF308 domain-containing protein -
  ACCO40_RS13030 (ACCO40_13030) nucA/comI 2548907..2549317 (-) 411 WP_014114486.1 sporulation-specific Dnase NucB Machinery gene
  ACCO40_RS13035 (ACCO40_13035) sigK 2549513..2550241 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  ACCO40_RS13040 (ACCO40_13040) - 2550317..2550398 (+) 82 Protein_2519 hypothetical protein -
  ACCO40_RS13045 (ACCO40_13045) - 2550364..2550561 (-) 198 Protein_2520 recombinase family protein -
  ACCO40_RS13050 (ACCO40_13050) - 2550519..2550697 (-) 179 Protein_2521 hypothetical protein -
  ACCO40_RS13055 (ACCO40_13055) - 2550880..2551947 (-) 1068 WP_372401708.1 isocitrate/isopropylmalate dehydrogenase family protein -
  ACCO40_RS13060 (ACCO40_13060) - 2552038..2552706 (-) 669 WP_372401710.1 aspartate/glutamate racemase family protein -
  ACCO40_RS13065 (ACCO40_13065) - 2553093..2553965 (-) 873 WP_114150356.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14938.98 Da        Isoelectric Point: 5.1643

>NTDB_id=1039433 ACCO40_RS13030 WP_014114486.1 2548907..2549317(-) (nucA/comI) [Bacillus spizizenii strain SHT-15]
MKKWMAGLFLAAAVLLCLMVPQQIQGASLYDKVLYFPLSRYPETGDHIKDAIADGHSDICTIDRDGADKRRQESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSGYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=1039433 ACCO40_RS13030 WP_014114486.1 2548907..2549317(-) (nucA/comI) [Bacillus spizizenii strain SHT-15]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
TTCTTTGTATGACAAAGTGTTGTATTTTCCGCTGTCTCGTTATCCGGAAACGGGGGATCATATTAAAGATGCGATTGCAG
ATGGTCATTCTGATATTTGCACTATCGACAGGGATGGAGCGGACAAAAGGCGGCAGGAATCATTAAAAGGAATTCCGACG
AAGCCGGGCTATGATCGGGATGAGTGGCCGATGGCGGTTTGTGAGGAAGGCGGCGCAGGAGCTGATGTCCGATATGTCAC
GCCTTCTGATAATCGCGGGGCCGGCTCGTGGGTAGGGAATCAAATGAGCGGTTATCCTGACGGCACCAGAGTGCTCTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment