Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACDI99_RS02165 Genome accession   NZ_CP167783
Coordinates   465549..466352 (+) Length   267 a.a.
NCBI ID   WP_005016505.1    Uniprot ID   A0A2T1J2S1
Organism   Acinetobacter radioresistens strain E3263934     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 460549..471352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDI99_RS02140 (ACDI99_02140) - 461476..462753 (+) 1278 WP_034670258.1 glycosyltransferase family 1 protein -
  ACDI99_RS02145 (ACDI99_02145) - 462782..463333 (+) 552 WP_005023384.1 phosphatase PAP2 family protein -
  ACDI99_RS02150 (ACDI99_02150) iscX 463421..463621 (+) 201 WP_005016518.1 Fe-S cluster assembly protein IscX -
  ACDI99_RS02155 (ACDI99_02155) ndk 463735..464166 (+) 432 WP_005016515.1 nucleoside-diphosphate kinase -
  ACDI99_RS02160 (ACDI99_02160) rlmN 464289..465524 (+) 1236 WP_119878352.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACDI99_RS02165 (ACDI99_02165) pilF 465549..466352 (+) 804 WP_005016505.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACDI99_RS02170 (ACDI99_02170) - 466343..467146 (+) 804 WP_034670256.1 helix-turn-helix domain-containing protein -
  ACDI99_RS02175 (ACDI99_02175) ispG 467169..468284 (+) 1116 WP_005016500.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACDI99_RS02180 (ACDI99_02180) hisS 468281..469576 (+) 1296 WP_005016497.1 histidine--tRNA ligase -
  ACDI99_RS02185 (ACDI99_02185) - 469608..470312 (+) 705 WP_005016495.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 30279.57 Da        Isoelectric Point: 9.5400

>NTDB_id=1039354 ACDI99_RS02165 WP_005016505.1 465549..466352(+) (pilF) [Acinetobacter radioresistens strain E3263934]
MRKPVYKTFVLLTVTLSALLVTACQTPDTLSKDPEKAVKVRTQLAAEYIKSGDLDAAKRALDQALEVDSRDATANMMMGV
LLQQEGSQLNLDKAESYFRRSISIEPKNAQARNNYGAYLYQIGRYKDAIEQLEIAGATLGYEQRYQALENLGRIYLKLGN
VANAEKTFKQALQANRDSSISMLELAEIFYLRQQIPAATQLYEQYVRRVGQKNQGARALWIGIRIARANDDKMGTQVLVN
QLRALFPDSQEYQRYLQLQHSTEAVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=1039354 ACDI99_RS02165 WP_005016505.1 465549..466352(+) (pilF) [Acinetobacter radioresistens strain E3263934]
TTGAGAAAGCCGGTGTATAAAACATTTGTATTACTTACAGTGACTTTGTCGGCCTTGCTTGTTACTGCTTGCCAGACTCC
TGATACCCTCAGTAAGGATCCAGAAAAGGCAGTCAAGGTTCGAACCCAGCTGGCAGCGGAATATATTAAATCGGGTGATC
TGGATGCTGCTAAACGGGCACTAGACCAGGCACTTGAAGTTGATTCCAGAGACGCCACTGCGAACATGATGATGGGAGTA
CTGCTCCAGCAAGAGGGGAGTCAGCTAAATCTGGACAAGGCTGAGAGCTATTTCAGACGCTCTATTTCAATTGAGCCTAA
AAATGCACAGGCGCGTAATAATTATGGCGCCTATTTGTACCAGATCGGTCGTTATAAAGATGCGATCGAACAACTGGAAA
TTGCTGGTGCAACTTTAGGCTATGAGCAGCGTTACCAGGCGCTTGAAAATCTGGGACGAATTTATCTTAAGCTCGGCAAT
GTTGCGAATGCTGAAAAAACATTCAAACAGGCATTGCAAGCTAACCGCGATTCTTCCATTTCAATGTTAGAGTTAGCGGA
AATTTTCTATTTGCGGCAACAGATTCCTGCTGCTACACAGCTTTATGAACAATATGTGCGTCGTGTAGGGCAAAAGAATC
AGGGTGCGCGTGCTCTTTGGATCGGAATTCGAATTGCGCGTGCCAATGATGATAAAATGGGAACACAAGTTTTGGTGAAC
CAGTTACGTGCCTTGTTCCCGGACAGCCAAGAATATCAACGTTATTTGCAATTACAGCACAGTACTGAGGCCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(44-82)

Tetratricopeptide repeat (TPR_16)

(150-208)

Tetratricopeptide repeat (TPR_17)

(98-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T1J2S1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.401

100

0.794
  pilF Acinetobacter baylyi ADP1

73.81

94.382

0.697

Multiple sequence alignment