Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACB328_RS08450 Genome accession   NZ_CP167123
Coordinates   1661074..1662435 (-) Length   453 a.a.
NCBI ID   WP_010921866.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain Isolate 32     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1656074..1667435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACB328_RS08430 (ACB328_08435) - 1656179..1657525 (-) 1347 WP_011285380.1 sensor histidine kinase -
  ACB328_RS08435 (ACB328_08440) gltX 1657920..1659365 (-) 1446 WP_002986105.1 glutamate--tRNA ligase -
  ACB328_RS08440 (ACB328_08445) - 1659547..1660257 (-) 711 WP_011017311.1 TIGR00266 family protein -
  ACB328_RS08445 (ACB328_08450) - 1660388..1660885 (-) 498 WP_010921867.1 carbonic anhydrase -
  ACB328_RS08450 (ACB328_08455) radA 1661074..1662435 (-) 1362 WP_010921866.1 DNA repair protein RadA Machinery gene
  ACB328_RS08455 (ACB328_08460) - 1662516..1662962 (-) 447 WP_002986111.1 dUTP diphosphatase -
  ACB328_RS08460 (ACB328_08465) - 1663072..1663839 (-) 768 WP_002986113.1 epoxyqueuosine reductase QueH -
  ACB328_RS08465 (ACB328_08470) - 1663957..1665741 (-) 1785 WP_010921865.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49372.61 Da        Isoelectric Point: 5.8179

>NTDB_id=1038409 ACB328_RS08450 WP_010921866.1 1661074..1662435(-) (radA) [Streptococcus pyogenes strain Isolate 32]
MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKNARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRV
LGGGVVPGSLILIGGDPGIGKSTLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNAR
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGEIGLTGEIR
RVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVGQVLNAVFS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1038409 ACB328_RS08450 WP_010921866.1 1661074..1662435(-) (radA) [Streptococcus pyogenes strain Isolate 32]
ATGGCAAAGAAAAAAGCAACCTTTATCTGTCAGGAGTGTGGCTACCAGTCGCCAAAGTATTTAGGGCGCTGTCCAAATTG
CTCGGCCTGGTCTTCTTTTGTGGAAGAAGTAGAGGTCAAAGAAGTCAAAAATGCCCGTGTTAGTCTAGCTGGAGAAAAGT
CCAGACCGGTTAAACTAAAAGATGTGGATAATATTAGTTATCACAGAACACAGACTGACATGTCTGAATTTAATAGAGTT
CTTGGAGGGGGTGTGGTTCCTGGAAGCTTGATTTTAATTGGTGGTGATCCAGGTATTGGAAAATCCACCTTACTCTTGCA
GGTATCCACGCAGCTCGCTAATAAGGGAACGGTTCTCTATGTGTCTGGGGAAGAATCAGCAGAGCAGATTAAACTGCGGA
GTGAGCGTCTGGGTGATATTGACAATGAATTTTACCTCTATGCCGAAACCAATATGCAAGCTATTCGCACAGAAATTGAA
AATATTAAACCAGATTTTTTAATTATTGACTCCATTCAGACCATCATGAGCCCTGATATTACAGGTGTTCAAGGATCAGT
CAGTCAGGTGAGAGAAGTCACTGCAGAATTGATGCAGCTTGCAAAGACGAACAACATTGCAACCTTTATTGTGGGTCATG
TCACCAAGGAAGGAACCTTGGCTGGGCCTCGTATGTTAGAACATATGGTGGATACCGTTCTCTACTTTGAAGGGGAAAGG
CATCACACCTTTCGCATTCTGCGGGCTGTGAAAAACCGCTTTGGCTCCACTAACGAGATTGGCATTTTTGAAATGCAGTC
TGGCGGCTTGGTAGAGGTCTTAAATCCTAGTCAAGTCTTTTTAGAGGAACGCTTAGATGGGGCAACAGGATCGGCTGTCG
TTGTAACGATGGAAGGAAGTCGTCCTATCTTAGCAGAGGTGCAATCTTTAGTGACACCAACCGTTTTTGGCAATGCCAGA
CGAACCACTACGGGACTTGATTTCAATCGCGTAAGCCTAATAATGGCAGTTTTAGAAAAGCGCTGTGGTTTACTCTTACA
AAATCAAGATGCCTACCTCAAGTCGGCTGGTGGTGTCAAACTAGACGAACCTGCCATTGATTTGGCAGTCGCTGTGGCTA
TCGCATCCAGTTATAAGGAAAAACCAACCAGCCCTCAAGAAGCTTTCTTAGGCGAGATCGGGTTAACAGGTGAGATTCGG
CGAGTGACCCGTATTGAGCAACGGATTAATGAAGCAGCAAAGCTTGGCTTTACCAAGGTCTATGCTCCTAAAAATGCCTT
GCAAGGCATTGATATCCCTCAAGGTATTGAGGTTGTCGGAGTGACTACGGTTGGTCAGGTTTTAAATGCAGTTTTTAGCT
AG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(56-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

85.651

100

0.857
  radA Streptococcus pneumoniae D39

85.43

100

0.854
  radA Streptococcus pneumoniae R6

85.43

100

0.854
  radA Streptococcus pneumoniae Rx1

85.43

100

0.854
  radA Streptococcus mitis SK321

85.43

100

0.854
  radA Streptococcus pneumoniae TIGR4

85.43

100

0.854
  radA Bacillus subtilis subsp. subtilis str. 168

61.504

99.779

0.614

Multiple sequence alignment