Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACB088_RS13675 Genome accession   NZ_CP167121
Coordinates   2889096..2889896 (-) Length   266 a.a.
NCBI ID   WP_003654874.1    Uniprot ID   -
Organism   Acinetobacter sp. RW6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2884096..2894896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACB088_RS13655 (ACB088_13655) - 2885161..2885859 (-) 699 WP_004641116.1 tetratricopeptide repeat protein -
  ACB088_RS13660 (ACB088_13660) hisS 2885888..2887180 (-) 1293 WP_017388395.1 histidine--tRNA ligase -
  ACB088_RS13665 (ACB088_13665) ispG 2887177..2888292 (-) 1116 WP_375037229.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACB088_RS13670 (ACB088_13670) - 2888314..2889105 (-) 792 WP_375037230.1 helix-turn-helix domain-containing protein -
  ACB088_RS13675 (ACB088_13675) pilF 2889096..2889896 (-) 801 WP_003654874.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACB088_RS13680 (ACB088_13680) rlmN 2889911..2891146 (-) 1236 WP_375037231.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACB088_RS13685 (ACB088_13685) ndk 2891270..2891701 (-) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  ACB088_RS13690 (ACB088_13690) iscX 2891815..2892015 (-) 201 WP_375037232.1 Fe-S cluster assembly protein IscX -
  ACB088_RS13695 (ACB088_13695) - 2892115..2892666 (-) 552 WP_375037233.1 phosphatase PAP2 family protein -
  ACB088_RS13700 (ACB088_13700) - 2892691..2893980 (-) 1290 WP_375038811.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30120.30 Da        Isoelectric Point: 9.0417

>NTDB_id=1038288 ACB088_RS13675 WP_003654874.1 2889096..2889896(-) (pilF) [Acinetobacter sp. RW6]
MSTPKLKTVFCMGVAVALLASGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAIEQFRIAGTTLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQSLLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1038288 ACB088_RS13675 WP_003654874.1 2889096..2889896(-) (pilF) [Acinetobacter sp. RW6]
TTGAGTACTCCAAAGTTAAAAACGGTATTTTGTATGGGAGTTGCAGTTGCGCTTTTGGCAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCAGAAAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCAGGTGACTTGG
ATTCGGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
CTACAGCAAGAAGGTAGTAAGCCGAACTTAGAAAAAGCAGAACATTATTTTAAACGAGCAATTTCATCTGAACCAGATAA
TGCTCAAGCACATAATAACTATGGTACTTATTTATACCAAATGGAACGCTATAATGATGCGATCGAGCAATTTCGTATTG
CTGGTACAACATTAGGTTATGACCAACGCTATCAGGCGTTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAGCAATCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAA
TTACGTGCCTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_16)

(89-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

94.737

100

0.947
  pilF Acinetobacter baylyi ADP1

75.385

97.744

0.737

Multiple sequence alignment