Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACB345_RS01165 Genome accession   NZ_CP167025
Coordinates   209461..210822 (+) Length   453 a.a.
NCBI ID   WP_002986109.1    Uniprot ID   A0A4Q1R665
Organism   Streptococcus pyogenes strain Isolate     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 176940..209380 209461..210822 flank 81


Gene organization within MGE regions


Location: 176940..210822
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACB345_RS01010 (ACB345_01010) polA 176940..179582 (+) 2643 WP_031488641.1 DNA polymerase I -
  ACB345_RS01015 (ACB345_01015) - 179760..180215 (+) 456 WP_031488640.1 CoA-binding protein -
  ACB345_RS01020 (ACB345_01020) perR 180267..180734 (+) 468 WP_031488639.1 peroxide-responsive transcriptional repressor PerR -
  ACB345_RS01025 (ACB345_01025) - 180953..181189 (+) 237 WP_014635252.1 hypothetical protein -
  ACB345_RS01030 (ACB345_01030) - 181411..182745 (+) 1335 WP_031488638.1 phosphoadenosine phosphosulfate reductase -
  ACB345_RS01035 (ACB345_01035) - 182738..183268 (+) 531 WP_002987925.1 ParB/RepB/Spo0J family partition protein -
  ACB345_RS01040 (ACB345_01040) - 183315..183464 (-) 150 Protein_157 ISL3 family transposase -
  ACB345_RS01045 (ACB345_01045) - 183596..184746 (-) 1151 Protein_158 IS3 family transposase -
  ACB345_RS01050 (ACB345_01050) - 184824..186134 (-) 1311 WP_031488784.1 SLC13 family permease -
  ACB345_RS01055 (ACB345_01055) nadC 186358..187230 (+) 873 WP_031488783.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACB345_RS01065 (ACB345_01065) - 188958..189821 (-) 864 WP_031488685.1 DUF975 family protein -
  ACB345_RS01070 (ACB345_01070) - 189858..190043 (+) 186 WP_002986321.1 hypothetical protein -
  ACB345_RS01075 (ACB345_01075) tgt 190040..191182 (+) 1143 WP_031488684.1 tRNA guanosine(34) transglycosylase Tgt -
  ACB345_RS01080 (ACB345_01080) - 191400..191711 (+) 312 WP_002987947.1 CHY zinc finger protein -
  ACB345_RS01085 (ACB345_01085) - 191715..192254 (+) 540 WP_002986314.1 biotin transporter BioY -
  ACB345_RS01090 (ACB345_01090) - 192394..193173 (+) 780 WP_011528209.1 MBL fold metallo-hydrolase -
  ACB345_RS01095 (ACB345_01095) tadA 193173..193688 (+) 516 WP_002992549.1 tRNA adenosine(34) deaminase TadA -
  ACB345_RS01100 (ACB345_01100) - 194302..195534 (-) 1233 WP_031488683.1 transglutaminase domain-containing protein -
  ACB345_RS01110 (ACB345_01110) speG 195934..196638 (+) 705 WP_031488682.1 streptococcal pyrogenic exotoxin SpeG -
  ACB345_RS01115 (ACB345_01115) - 197094..198443 (+) 1350 WP_014635263.1 glucose-6-phosphate isomerase -
  ACB345_RS01120 (ACB345_01120) - 198792..200300 (-) 1509 WP_002986134.1 helix-turn-helix domain-containing protein -
  ACB345_RS01125 (ACB345_01125) - 200988..201659 (+) 672 WP_031488681.1 rhomboid family intramembrane serine protease -
  ACB345_RS01130 (ACB345_01130) galU 201758..202657 (-) 900 WP_002986125.1 UTP--glucose-1-phosphate uridylyltransferase GalU -
  ACB345_RS01135 (ACB345_01135) - 202690..203706 (-) 1017 WP_002986123.1 NAD(P)H-dependent glycerol-3-phosphate dehydrogenase -
  ACB345_RS01140 (ACB345_01140) - 204004..204453 (+) 450 WP_002986120.1 MarR family winged helix-turn-helix transcriptional regulator -
  ACB345_RS01145 (ACB345_01145) - 204446..206152 (+) 1707 WP_031488680.1 ABC transporter ATP-binding protein -
  ACB345_RS01150 (ACB345_01150) - 206155..207939 (+) 1785 WP_023610145.1 ABC transporter ATP-binding protein -
  ACB345_RS01155 (ACB345_01155) - 208057..208824 (+) 768 WP_011054158.1 epoxyqueuosine reductase QueH -
  ACB345_RS01160 (ACB345_01160) - 208934..209380 (+) 447 WP_002986111.1 dUTP diphosphatase -
  ACB345_RS01165 (ACB345_01165) radA 209461..210822 (+) 1362 WP_002986109.1 DNA repair protein RadA Machinery gene

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49386.68 Da        Isoelectric Point: 6.0962

>NTDB_id=1038119 ACB345_RS01165 WP_002986109.1 209461..210822(+) (radA) [Streptococcus pyogenes strain Isolate]
MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKNARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRV
LGGGVVPGSLILIGGDPGIGKSTLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNAR
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGEIGLTGEIR
RVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVGQVLKAVFS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1038119 ACB345_RS01165 WP_002986109.1 209461..210822(+) (radA) [Streptococcus pyogenes strain Isolate]
ATGGCAAAGAAAAAAGCAACCTTTATCTGTCAGGAGTGTGGCTACCAGTCGCCAAAATATTTAGGGCGCTGTCCAAATTG
CTCGGCCTGGTCTTCTTTTGTGGAAGAAGTAGAGGTCAAAGAAGTCAAAAATGCCCGTGTTAGTCTAGCTGGAGAAAAGT
CCAGACCGGTTAAACTAAAAGATGTGGATAATATTAGTTATCACAGAACACAGACTGACATGTCTGAATTTAATAGAGTT
CTTGGAGGGGGTGTGGTTCCTGGAAGCTTGATTTTAATTGGTGGTGATCCGGGTATTGGAAAATCCACCTTACTCTTGCA
GGTATCCACGCAGCTCGCTAATAAGGGAACGGTTCTCTATGTGTCTGGGGAAGAATCAGCAGAGCAGATTAAACTGCGGA
GTGAGCGTCTGGGTGATATTGACAATGAATTTTACCTCTATGCCGAAACCAATATGCAAGCTATTCGCACAGAAATTGAA
AATATTAAACCAGATTTTTTAATTATTGACTCCATTCAGACCATCATGAGCCCTGATATTACAGGTGTTCAAGGATCAGT
CAGTCAGGTGAGAGAAGTCACTGCAGAATTGATGCAGCTTGCAAAGACGAACAATATTGCAACCTTTATTGTGGGTCATG
TCACCAAGGAAGGAACCTTGGCTGGGCCTCGTATGTTAGAACACATGGTGGATACCGTTCTCTACTTTGAAGGGGAAAGG
CATCACACCTTTCGCATTCTTCGGGCTGTGAAAAACCGCTTTGGCTCCACTAACGAGATCGGCATCTTTGAAATGCAGTC
TGGCGGCTTGGTAGAGGTCTTAAATCCTAGTCAAGTCTTTTTAGAGGAACGCTTAGATGGGGCAACAGGATCGGCTGTCG
TTGTAACGATGGAAGGAAGTCGTCCTATCTTAGCAGAGGTGCAATCTTTAGTGACACCAACCGTTTTTGGCAATGCCAGA
CGAACCACTACGGGACTTGATTTCAATCGCGTAAGCCTAATAATGGCAGTTTTAGAAAAGCGCTGTGGTTTACTCTTACA
AAATCAAGATGCCTACCTCAAGTCGGCTGGTGGTGTCAAACTAGACGAACCTGCCATTGATTTGGCAGTCGCTGTGGCTA
TCGCATCCAGTTATAAGGAAAAACCAACCAGCCCTCAAGAAGCTTTCTTAGGCGAGATCGGGTTAACAGGTGAGATTCGG
CGAGTGACCCGTATTGAGCAACGGATTAATGAAGCAGCAAAGCTTGGATTTACCAAGGTCTATGCTCCTAAAAATGCCTT
GCAAGGCATTGATATCCCTCAAGGTATTGAGGTTGTCGGAGTGACTACGGTTGGTCAGGTTTTAAAGGCAGTTTTTAGCT
AG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(56-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Q1R665

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

85.872

100

0.859
  radA Streptococcus pneumoniae D39

85.651

100

0.857
  radA Streptococcus pneumoniae R6

85.651

100

0.857
  radA Streptococcus pneumoniae Rx1

85.651

100

0.857
  radA Streptococcus mitis SK321

85.651

100

0.857
  radA Streptococcus pneumoniae TIGR4

85.651

100

0.857
  radA Bacillus subtilis subsp. subtilis str. 168

61.369

100

0.614

Multiple sequence alignment