Detailed information    

experimental Experimentally validated

Overview

Name   comZ   Type   Machinery gene
Locus tag   TT_RS04330 Genome accession   NC_005835
Coordinates   833222..834886 (+) Length   554 a.a.
NCBI ID   WP_011173286.1    Uniprot ID   Q72JC1
Organism   Thermus thermophilus HB27     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

ComZ and a ComZ/PilA2 complex interact with dsDNA which led to the suggestion that PilA2 assists ComZ in forming a competence pilus tip binding DNA.


Genomic Context

Location: 828222..839886
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TT_RS04305 (TT_C0852) ilvB 828447..830135 (-) 1689 WP_011173281.1 biosynthetic-type acetolactate synthase large subunit -
  TT_RS04310 (TT_C0853) - 830290..831270 (-) 981 WP_011173282.1 YpdA family putative bacillithiol disulfide reductase -
  TT_RS04315 (TT_C0854) pilA/pilA1 831428..831898 (+) 471 WP_011173283.1 GspH/FimT family protein Machinery gene
  TT_RS04320 (TT_C0855) pilA/pilA2 831932..832513 (+) 582 WP_011173284.1 type IV pilus modification PilV family protein Machinery gene
  TT_RS04325 (TT_C0856) pilA/pilA3 832510..833211 (+) 702 WP_011173285.1 PilW family protein Machinery gene
  TT_RS04330 (TT_C0857) comZ 833222..834886 (+) 1665 WP_011173286.1 pilus assembly PilX N-terminal domain-containing protein Machinery gene
  TT_RS11945 (TT_C0858) pilA/pilA4 835117..835512 (+) 396 WP_011173287.1 type IV wide pilus major component PilA4 Machinery gene
  TT_RS11645 - 835569..836861 (-) 1293 WP_143591263.1 O-antigen ligase family protein -
  TT_RS04345 (TT_C0859) - 837118..837630 (+) 513 WP_011173288.1 hypothetical protein -
  TT_RS04350 (TT_C0860) - 837706..838248 (+) 543 WP_011173289.1 Uma2 family endonuclease -
  TT_RS04355 (TT_C0861) - 838286..838486 (+) 201 WP_011173290.1 DUF2281 domain-containing protein -
  TT_RS04360 (TT_C0862) - 838585..839142 (-) 558 WP_011173291.1 Uma2 family endonuclease -
  TT_RS04365 (TT_C0863) - 839165..839821 (-) 657 WP_011173292.1 chromosome segregation protein ScpA -

Sequence

Protein


Download         Length: 554 a.a.        Molecular weight: 59298.84 Da        Isoelectric Point: 7.7127

>NTDB_id=1036 TT_RS04330 WP_011173286.1 833222..834886(+) (comZ) [Thermus thermophilus HB27]
MNAKGIALVATLALMVVIALLVFGTFFTTQIELWTTRNDTTSVQAFYAAEAGLQKYKAALFQQYVWREQRGGTGGGGGCF
TSLARGLDLDRDGTITPFVNNRLVLAQNEVVTDANGNPVGRYTATLYKDAQDDQLFTLVSEGTSGGAKARVQATFRISNS
DYLEQAIFAGAGQANKWLNGGATIRGGVYVVGNPNDPDQYVIEANGNFALYNRYDLTTYSEVTNRVEPSYRQVQDLCASL
RVQYGKISVGGSTQIGEPNNKVKGVFVGRGAQDITGENVGVCRNNKGVCTEAMGGFDLSDPPPFPTLDAKLDSDACSAYP
TWRACLQGKAALRIQRIGNILSVASPPNATLSPSCLQAMQSGTLTLDTQSVDCTFTRLDGSRGGFRYTYTGGQELLEVFG
DVVLEGIDAVLNRPVDYRAQSGSAKSATLAVLKLGGNGGNLDINGNLLPDATFGLFPNHALGFVAEGDIYQRGQHVMAPV
YAGGTFRVVKGNVLFGSVISNQFCTTSAGNQMSCNASQKAEVVYIRIPKENRPALLPSLRGGKPVFQVLSYERR

Nucleotide


Download         Length: 1665 bp        

>NTDB_id=1036 TT_RS04330 WP_011173286.1 833222..834886(+) (comZ) [Thermus thermophilus HB27]
GTGAACGCGAAAGGTATCGCCCTCGTAGCCACTTTGGCCCTCATGGTGGTCATCGCCCTCTTGGTCTTCGGCACCTTCTT
CACCACCCAGATAGAGCTCTGGACCACCCGCAACGACACCACCTCGGTCCAGGCCTTCTACGCCGCCGAGGCGGGGTTGC
AGAAGTACAAGGCTGCCCTCTTCCAGCAGTACGTCTGGCGGGAGCAGCGGGGCGGGACGGGGGGAGGTGGGGGCTGCTTC
ACCTCCCTGGCAAGGGGGCTGGACCTGGACCGGGATGGAACCATCACGCCCTTCGTCAACAACCGGCTGGTCCTGGCGCA
AAACGAGGTGGTCACCGACGCCAACGGAAACCCCGTGGGGCGGTACACGGCAACCCTTTACAAGGACGCCCAAGACGACC
AGCTCTTCACCCTGGTCTCCGAGGGCACCTCGGGCGGGGCCAAGGCCCGGGTCCAGGCCACCTTCCGCATCAGCAACTCG
GACTACCTGGAGCAGGCCATCTTCGCCGGCGCGGGCCAGGCGAACAAGTGGCTGAACGGCGGGGCCACCATCCGGGGCGG
GGTCTATGTGGTGGGAAACCCTAACGACCCGGACCAGTACGTCATAGAGGCCAACGGCAACTTTGCCCTCTACAACCGGT
ACGACCTCACCACCTACTCCGAGGTCACCAACCGGGTGGAACCCAGCTACCGGCAGGTCCAGGACCTCTGCGCAAGCCTT
AGGGTGCAGTACGGCAAGATTTCCGTGGGAGGAAGCACCCAGATCGGCGAGCCCAACAACAAGGTGAAGGGAGTCTTCGT
GGGCCGGGGTGCCCAGGACATCACCGGCGAGAACGTGGGGGTCTGCCGGAACAACAAAGGGGTCTGCACCGAGGCCATGG
GGGGCTTTGACCTCTCCGACCCGCCCCCCTTCCCCACCCTGGACGCCAAGCTGGATTCCGACGCCTGCAGCGCCTACCCC
ACCTGGCGGGCCTGCCTCCAGGGCAAGGCCGCTTTAAGAATCCAGCGCATTGGGAACATCCTCTCCGTGGCTAGCCCGCC
TAACGCCACCTTGTCCCCCTCCTGCCTCCAGGCCATGCAGTCCGGTACCCTGACCCTGGACACCCAAAGCGTGGACTGCA
CCTTCACCCGTCTGGACGGAAGCCGGGGAGGGTTCAGGTACACCTACACGGGCGGGCAGGAGCTTTTGGAGGTCTTCGGC
GACGTGGTGCTGGAGGGCATAGACGCGGTCCTCAACCGGCCCGTGGACTATAGGGCCCAGTCGGGAAGCGCCAAGAGCGC
CACCCTTGCGGTGCTGAAGCTTGGCGGCAACGGCGGCAATTTGGACATCAACGGCAACCTCCTCCCCGACGCCACCTTTG
GCCTCTTCCCCAACCACGCCCTGGGCTTCGTGGCCGAAGGGGACATCTACCAGCGCGGCCAGCACGTGATGGCCCCCGTG
TACGCCGGGGGCACCTTCCGGGTGGTGAAGGGCAACGTCCTCTTCGGCTCCGTCATTAGCAACCAGTTCTGCACCACAAG
CGCCGGGAACCAAATGAGCTGCAACGCCAGCCAGAAGGCCGAGGTGGTCTACATCCGCATCCCCAAGGAGAACCGCCCCG
CGCTCCTGCCGAGCCTCCGCGGGGGGAAGCCCGTCTTTCAGGTGCTCTCCTATGAGCGCCGCTAG

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 6QVI

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Beate Averhoff et al. (2021) Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation. Extremophiles : Life Under Extreme Conditions 25(5-6):425-436. [PMID: 34542714]
[2] Mohd Zulkifli Salleh et al. (2019) Structure and Properties of a Natural Competence-Associated Pilin Suggest a Unique Pilus Tip-Associated DNA Receptor. MBio 10(3):e00614-19. [PMID: 31186316]
[3] Alexandra Friedrich et al. (2003) Pilin-like proteins in the extremely thermophilic bacterium Thermus thermophilus HB27: implication in competence for natural transformation and links to type IV pilus biogenesis. Applied And Environmental Microbiology 69(7):3695-700. [PMID: 12839734]