Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACA109_RS10180 Genome accession   NZ_CP166872
Coordinates   2009133..2010497 (-) Length   454 a.a.
NCBI ID   WP_371256666.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 24EBSta0529     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2004133..2015497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACA109_RS10165 (ACA109_10165) cysE 2004954..2005601 (-) 648 WP_001805237.1 serine O-acetyltransferase -
  ACA109_RS10170 (ACA109_10170) gltX 2006024..2007478 (-) 1455 WP_001283789.1 glutamate--tRNA ligase -
  ACA109_RS10175 (ACA109_10175) - 2008035..2009108 (-) 1074 WP_001028375.1 PIN/TRAM domain-containing protein -
  ACA109_RS10180 (ACA109_10180) radA 2009133..2010497 (-) 1365 WP_371256666.1 DNA repair protein RadA Machinery gene
  ACA109_RS10185 (ACA109_10185) - 2010983..2013439 (-) 2457 WP_000897132.1 ATP-dependent Clp protease ATP-binding subunit -
  ACA109_RS10190 (ACA109_10190) - 2013453..2014460 (-) 1008 WP_000149505.1 protein arginine kinase -
  ACA109_RS10195 (ACA109_10195) - 2014450..2015016 (-) 567 WP_000882069.1 UvrB/UvrC motif-containing protein -
  ACA109_RS10200 (ACA109_10200) - 2015035..2015496 (-) 462 WP_000551762.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49849.38 Da        Isoelectric Point: 7.0049

>NTDB_id=1035853 ACA109_RS10180 WP_371256666.1 2009133..2010497(-) (radA) [Staphylococcus aureus strain 24EBSta0529]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPKHGVKTKELAGKIQKLNSIKHETTPRVLTDSAEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSFALHS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=1035853 ACA109_RS10180 WP_371256666.1 2009133..2010497(-) (radA) [Staphylococcus aureus strain 24EBSta0529]
TTGGCCAAGAAAAAAGTGATTTTTGAATGTATGGCTTGTGGTTATCAATCTCCTAAATGGATGGGGAAATGTCCTAATTG
TGGCGCTTGGAATCAAATGGAGGAAATTGTTGAAAAAGCAGCCAATCCTAAACATGGAGTGAAAACCAAGGAATTAGCAG
GTAAAATACAAAAATTAAATAGTATTAAACATGAAACAACGCCGAGAGTGTTAACAGATTCAGCAGAATTCAACCGTGTA
TTAGGTGGAGGTATTGTGAGTGGATCGTTAGTACTTATTGGTGGGGATCCAGGTATTGGTAAGTCAACGTTACTTTTACA
AATTTGTGCATCGTTATCTCAAAAGAAAAAAGTACTATATATTACTGGAGAAGAATCGCTTAGTCAGACTAAATTACGTG
CAGAGCGATTAGATGAAGATTCAAGTGAATTGCAAGTATTAGCTGAAACAGATCTTGAAGTTATTTATCAAACAGTAAAA
GAAGAACAACCTGATTTATTAGTAGTGGATTCGATTCAAACAATATATCATCCTGAAATCAGCTCTGCGCCAGGTTCTGT
TTCACAAGTTCGTGAAAGTACACAAAGTTTAATGAATATTGCTAAACAAATGAACATTGCAACTTTTATAGTGGGTCATG
TAACGAAAGAAGGTCAAATTGCTGGCCCAAGATTGCTAGAACACATGGTTGATACTGTGCTTTATTTTGAAGGCGATGAA
CACCACGCATATCGAATTTTGCGAGCTGTTAAAAACCGTTTTGGTTCAACGAATGAAATGGGAATCTTCGAAATGAAGCA
AAGTGGATTAAAAGGTGTAAATAATCCATCTGAAATGTTTTTAGAAGAACGTTCAACAAATGTTCCAGGTTCAACAATTG
TTGCAACCATGGAGGGAACCAGACCACTTTTAATAGAAGTTCAAGCGCTGGTAACTCCAACGACTTTTAACAATCCGAGA
CGAATGGCAACAGGGATTGATCATAATCGATTAAGTTTGTTGATGGCTGTTTTGGAAAAGAAAGAAAATTATCTATTACA
ACAACAAGATGCTTATATCAAAGTAGCTGGCGGTGTAAAGTTAACGGAGCCAGCAGTTGATTTAAGTGTAATTGTAGCAA
CTGCGTCTAGCTTTAAAGATAAAGCTGTCGACGGATTAGATTGCTATATTGGAGAAGTTGGTTTAACGGGTGAGGTACGT
CGTGTATCTCGGATAGAACAACGCGTGCAAGAGGCTGCAAAACTAGGTTTCAAACGTGTAATTATTCCTAAAAATAATAT
AGGCGGATGGACATATCCTGAAGGTATACAAGTAATAGGTGTAACTACTGTACATGAAGCATTGTCATTTGCTCTTCATT
CATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)

AAA domain (AAA_25)

(77-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.667

100

0.674
  radA Streptococcus pneumoniae Rx1

62.054

98.678

0.612
  radA Streptococcus pneumoniae D39

62.054

98.678

0.612
  radA Streptococcus pneumoniae R6

62.054

98.678

0.612
  radA Streptococcus pneumoniae TIGR4

62.054

98.678

0.612
  radA Streptococcus mitis NCTC 12261

62.054

98.678

0.612
  radA Streptococcus mitis SK321

62.054

98.678

0.612

Multiple sequence alignment