Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AB3239_RS13865 Genome accession   NZ_CP166831
Coordinates   2652457..2652867 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain TE3T-UV25     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2647457..2657867
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3239_RS13835 (AB3239_13835) - 2647543..2647683 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  AB3239_RS13840 (AB3239_13840) yqeF 2647989..2648720 (-) 732 WP_041052434.1 SGNH/GDSL hydrolase family protein -
  AB3239_RS13845 (AB3239_13845) cwlH 2648972..2649724 (-) 753 WP_085186516.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  AB3239_RS13850 (AB3239_13850) yqeD 2649911..2650537 (+) 627 WP_172861316.1 TVP38/TMEM64 family protein -
  AB3239_RS13855 (AB3239_13855) gnd 2650557..2651450 (-) 894 WP_046160611.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  AB3239_RS13860 (AB3239_13860) yqeB 2651702..2652424 (+) 723 WP_021480070.1 hypothetical protein -
  AB3239_RS13865 (AB3239_13865) nucA/comI 2652457..2652867 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  AB3239_RS13870 (AB3239_13870) sigK 2653063..2653791 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  AB3239_RS13875 (AB3239_13875) - 2653791..2653907 (+) 117 WP_070547560.1 hypothetical protein -
  AB3239_RS13880 (AB3239_13880) - 2653873..2654070 (-) 198 Protein_2687 recombinase family protein -
  AB3239_RS13885 (AB3239_13885) - 2654028..2654181 (-) 154 Protein_2688 hypothetical protein -
  AB3239_RS13890 (AB3239_13890) psiE 2654727..2655143 (-) 417 WP_003246154.1 phosphate-starvation-inducible protein PsiE -
  AB3239_RS13895 (AB3239_13895) yrkQ 2655189..2656487 (-) 1299 WP_370956882.1 two-component system sensor histidine kinase YrkQ -
  AB3239_RS13900 (AB3239_13900) yrkP 2656474..2657169 (-) 696 WP_021480140.1 two-component response regulator YrkP -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=1035543 AB3239_RS13865 WP_009967785.1 2652457..2652867(-) (nucA/comI) [Bacillus subtilis strain TE3T-UV25]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=1035543 AB3239_RS13865 WP_009967785.1 2652457..2652867(-) (nucA/comI) [Bacillus subtilis strain TE3T-UV25]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment