Detailed information NTG0001035

Experimentally validated

Overview

• Name: comA/comEC
• Type: Machinery gene
• Locus tag: TT_RS08105
• Genome accession: NC_005835
• Coordinates: 1516868..1518901 (+)
• Length: 677 a.a.
• NCBI ID: WP_011173974.1
• Uniprot ID: Q72H92
• Organism: Thermus thermophilus HB27
• Function: ssDNA transport through the inner membrane DNA binding and uptake

Function

In addition to the pseudopilus the IM anchored ComEA protein which binds dsDNA is important for the transport of DNA into a DNase-resistant state by pulling the DNA through the OM. The ssDNA is subsequently transported across the IM through a channel formed by the polytopic IM protein ComEC (ComA homologue). Interestingly, ComEC was also found to modulate transcriptional regulation of DNA transporter and T4P components.

Genomic Context

Location: 1511868..1523901

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
TT_RS08070 (TT_C1596)	-	1512644..1513108 (+)	465	WP_011173967.1	hypothetical protein	-
TT_RS08075 (TT_C1597)	pfkA	1513085..1514053 (-)	969	WP_011173968.1	6-phosphofructokinase	-
TT_RS08080 (TT_C1598)	rsmI	1514050..1514844 (-)	795	WP_011173969.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-
TT_RS08085 (TT_C1599)	-	1514846..1515340 (-)	495	WP_011173970.1	hypothetical protein	-
TT_RS08090 (TT_C1600)	-	1515404..1515931 (-)	528	WP_011173971.1	inorganic diphosphatase	-
TT_RS08095 (TT_C1601)	-	1515964..1516590 (+)	627	WP_011173972.1	YcxB family protein	-
TT_RS08100 (TT_C1602)	comEA	1516563..1516871 (+)	309	WP_024119555.1	ComEA family DNA-binding protein	Machinery gene
TT_RS08105 (TT_C1603)	comA/comEC	1516868..1518901 (+)	2034	WP_011173974.1	ComEC/Rec2 family competence protein	Machinery gene
TT_RS08110 (TT_C1604)	-	1518889..1519698 (-)	810	WP_011173975.1	ParB/RepB/Spo0J family partition protein	-
TT_RS08115 (TT_C1605)	soj	1519682..1520431 (-)	750	WP_011173976.1	chromosome-partitioning ATPase Soj	-
TT_RS08120 (TT_C1606)	rsmG	1520425..1521174 (-)	750	WP_011173977.1	16S rRNA (guanine(527)-N(7))-methyltransferase RsmG	-
TT_RS08125 (TT_C1607)	mnmG	1521174..1522967 (-)	1794	WP_011173978.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG	-

Sequence

Protein

• Length: 677 a.a.
• Molecular weight: 71948.00 Da
• Isoelectric Point: 9.9521

>NTDB_id=1035 TT_RS08105 WP_011173974.1 1516868..1518901(+) (comA/comEC) [Thermus thermophilus HB27]
MRPSWTWGVGLGAGALVAAWGLFAPWAFLGLGLLPFLRLPFALGFGLVLARALFFPLPEPPWGAAVEGVFAVRGGFTRAE
GHLLWVSHYPPLPDGRYRLRGRIAPPQGRTNPGGFDQRAWLLSRGAKGVLRAESAEPLGPLPDWREGFRERLGAGLSLEA
REVVEGLVLGDKGGLETAYPLFQRAGLAHLLALSGLHVGVLVGFAVLGLYPLGRWRYLLALALLPFYLLLAGPSPSLVRA
SLMAGLSLLGLFLGLGGAGVVQALGLALFLQLLLRPEALLGLGFQLSYLAVLGLALVLPALRLPPGPRGYLLGGVAASLA
VQAFLLPLLLHRFGFAPLLAPLANLLALPLVALLVPLGFLKLFLGALPAPLVEPLARGLLLLAGLAAQGPLLRWGEIAPP
GFALYYLGLLPLLFALHRRLPWRRALLLASLPALVGLLAAWPKPLDLWMLDVGQGDALLARMGGGEVLVDGGRAEKGEAV
VRALRALGVEALELLVATHPDADHYGGLFRVIEEVPVGLALLAPGFPEDHPLAEALRARGVPVVRAGAGTRLKVGQGEVR
VLWPEALSGDDNQDGLALLLDFGRGRALLLADLPREVEARLPVGEVEALKVSHHGSQSGTSEVLLERTRPRVALIGVGPN
PFGHPHPEVLERLERHGAEIRRTDQDGAVRVLFGYAW

Nucleotide

• Length: 2034 bp

>NTDB_id=1035 TT_RS08105 WP_011173974.1 1516868..1518901(+) (comA/comEC) [Thermus thermophilus HB27]
ATGAGGCCTTCTTGGACCTGGGGAGTGGGGCTTGGGGCGGGGGCTTTGGTGGCCGCCTGGGGGCTTTTCGCGCCTTGGGC
CTTCCTTGGGCTCGGCCTCCTTCCCTTCCTCCGCCTTCCCTTCGCCCTGGGCTTCGGCCTCGTCCTGGCCCGGGCCCTTT
TCTTCCCCTTGCCCGAGCCCCCGTGGGGCGCCGCCGTGGAGGGGGTCTTCGCCGTGAGGGGCGGGTTCACACGGGCGGAG
GGCCACCTCCTTTGGGTGAGCCACTATCCCCCTCTACCGGACGGCCGCTACCGCCTCCGGGGGCGCATCGCCCCGCCCCA
AGGCCGGACCAACCCCGGGGGGTTTGACCAACGGGCCTGGCTCCTGAGCCGGGGGGCCAAGGGGGTGCTCCGGGCCGAAA
GCGCCGAGCCCTTGGGGCCCCTGCCCGACTGGAGGGAGGGGTTCCGGGAACGCCTAGGGGCGGGCCTGAGCCTCGAGGCC
CGGGAGGTGGTGGAGGGCCTGGTCCTCGGGGACAAGGGGGGCTTAGAGACGGCCTACCCCCTCTTCCAGCGGGCGGGCCT
CGCCCACCTCCTGGCGCTCTCTGGCCTTCACGTGGGGGTGCTCGTGGGCTTCGCCGTCCTCGGCCTCTACCCCCTGGGGC
GGTGGCGCTACCTCCTGGCCCTCGCCCTCCTCCCCTTTTACCTCCTCCTCGCCGGGCCGAGCCCCTCCCTGGTGCGGGCG
AGCCTCATGGCGGGCCTGTCCCTCCTCGGCCTCTTCCTGGGCCTTGGGGGGGCCGGGGTGGTCCAGGCCCTGGGGCTCGC
CCTCTTCCTCCAGCTCCTCCTCCGCCCCGAGGCCCTCCTCGGCCTCGGGTTCCAGCTCTCCTACCTCGCGGTGCTGGGCC
TGGCCCTGGTCCTCCCGGCCCTGAGGCTTCCCCCGGGCCCCAGGGGCTATCTCCTCGGGGGCGTGGCCGCAAGCCTGGCC
GTCCAGGCCTTCCTCCTCCCCCTCCTCCTCCACCGCTTCGGCTTCGCCCCCCTCCTCGCCCCCCTCGCCAACCTCCTCGC
CCTCCCCCTGGTGGCCCTCCTCGTGCCCCTCGGCTTCCTCAAGCTCTTCCTCGGCGCCCTCCCCGCCCCCCTGGTGGAGC
CCCTCGCCCGGGGGCTCCTCCTCCTGGCGGGGCTTGCGGCCCAAGGGCCCCTCCTCCGCTGGGGGGAGATCGCCCCCCCA
GGCTTTGCCCTCTACTACCTGGGCCTTCTCCCCCTCCTTTTCGCCCTCCACCGCAGGCTTCCCTGGCGGCGCGCCCTCCT
CCTCGCCTCCCTGCCCGCCCTGGTGGGCCTCCTCGCCGCCTGGCCCAAGCCGCTGGACCTCTGGATGCTGGACGTGGGCC
AGGGGGACGCCCTCCTCGCCCGCATGGGGGGCGGGGAGGTCCTGGTGGACGGGGGACGGGCGGAGAAGGGGGAGGCGGTG
GTCCGGGCCCTCCGCGCCCTCGGGGTGGAGGCCCTGGAGCTTTTGGTGGCCACCCACCCCGACGCGGACCACTACGGGGG
GCTTTTCCGGGTGATAGAGGAGGTTCCCGTGGGCCTTGCCCTCCTCGCCCCCGGCTTCCCCGAGGACCACCCCCTGGCCG
AGGCCCTAAGGGCGCGGGGCGTCCCCGTGGTGCGGGCCGGGGCGGGAACCCGCCTTAAGGTGGGCCAGGGCGAGGTCCGG
GTGCTCTGGCCCGAGGCCCTCTCCGGGGACGACAACCAAGACGGCCTCGCCCTCCTCCTGGACTTCGGCCGGGGACGGGC
CCTCCTCCTCGCCGACCTGCCCCGGGAGGTGGAGGCCAGGCTTCCCGTGGGGGAGGTGGAGGCGCTCAAGGTGAGCCACC
ACGGCTCCCAGAGCGGCACCTCCGAGGTCCTCCTGGAAAGGACGAGGCCCCGGGTGGCCCTCATCGGGGTGGGGCCCAAC
CCCTTCGGCCACCCCCACCCCGAGGTCCTGGAGCGCCTTGAGCGCCACGGCGCGGAGATACGCCGCACCGACCAGGACGG
GGCGGTGCGCGTCCTCTTCGGCTACGCCTGGTAG

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (451-635)

• Competence protein (Competence) (167-418)

• Domain of unknown function (DUF4131) (DUF4131) (93-136)

3D structure

• AlphaFold DB: Q72H92

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.85	90.399	0.369

References

• [1]: Beate Averhoff et al. (2021) Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation. Extremophiles : Life Under Extreme Conditions 25(5-6):425-436. [PMID: 34542714]
• [2]: Ralf Salzer et al. (2016) The Thermus thermophilus comEA/comEC operon is associated with DNA binding and regulation of the DNA translocator and type IV pili. Environmental Microbiology 18(1):65-74. [PMID: 25727469]
• [3]: Cornelia Schwarzenlander et al. (2009) The role of single subunits of the DNA transport machinery of Thermus thermophilus HB27 in DNA binding and transport. Environmental Microbiology 11(4):801-8. [PMID: 19396940]