Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   AB9V60_RS01645 Genome accession   NZ_CP166624
Coordinates   343447..345666 (-) Length   739 a.a.
NCBI ID   WP_003422549.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. atrofaciens strain GM 2231     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 338447..350666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB9V60_RS01625 (AB9V60_01625) hslU 338567..339904 (-) 1338 WP_003316641.1 ATP-dependent protease ATPase subunit HslU -
  AB9V60_RS01630 (AB9V60_01630) hslV 339971..340501 (-) 531 WP_003392345.1 ATP-dependent protease subunit HslV -
  AB9V60_RS01635 (AB9V60_01635) - 340916..341611 (-) 696 WP_004414617.1 SPOR domain-containing protein -
  AB9V60_RS01640 (AB9V60_01640) argS 341613..343349 (-) 1737 WP_003432149.1 arginine--tRNA ligase -
  AB9V60_RS01645 (AB9V60_01645) priA 343447..345666 (-) 2220 WP_003422549.1 primosomal protein N' Machinery gene
  AB9V60_RS01650 (AB9V60_01650) rpmE 345836..346057 (+) 222 WP_002551648.1 50S ribosomal protein L31 -
  AB9V60_RS01655 (AB9V60_01655) - 346071..346874 (+) 804 WP_134943089.1 thermonuclease family protein -
  AB9V60_RS01660 (AB9V60_01660) - 347200..348468 (+) 1269 WP_370683282.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81901.20 Da        Isoelectric Point: 8.4231

>NTDB_id=1034835 AB9V60_RS01645 WP_003422549.1 343447..345666(-) (priA) [Pseudomonas syringae pv. atrofaciens strain GM 2231]
MPDAILRLALPSPLRRLFDYRAPAGVSRSALQPGMRLRVPFGRREMIGILVEVVDHSEVPADKLKPAIALLDSEAPLPPA
LFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAESRQERFWHVTPGASVDDPRIARAPKQREALTTLAQHPHGVAHQLL
SKLMLNKDSLNLLLAKELVYVEVRSHAPSARHEHWLAQPELPLNTEQRAAYEAIRAGFDSFHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALVHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNERAGGAQQPRFMRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSGCQRCDARMTVHQRSGELRCHHCGYVERVPRQC
PSCGKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFTTIQRGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLIQLTEQGYFAFAEQALSERRSAGLPPFSHL
ALLRAEAHKPGQAEAFLDQACSDAEQLVAQMALGGIELLGPVPAPMERRAGRYRAQLLVQSSARAPLHKLLATWLLALEQ
MPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=1034835 AB9V60_RS01645 WP_003422549.1 343447..345666(-) (priA) [Pseudomonas syringae pv. atrofaciens strain GM 2231]
GTGCCCGACGCCATCCTGCGCCTTGCCCTGCCCTCGCCGCTTCGCCGCCTGTTCGACTACCGCGCACCAGCGGGCGTGTC
GCGCAGCGCCTTGCAGCCGGGCATGCGTCTGCGAGTGCCGTTCGGACGCCGGGAAATGATCGGCATCCTGGTGGAAGTGG
TCGATCACAGCGAAGTGCCCGCCGACAAGCTCAAGCCGGCGATCGCCCTGCTCGACAGCGAAGCGCCGCTGCCACCTGCG
CTGTTCAAGCTGTGCCTGTGGACCTCGCAGTACTACCAGCACAGCCTCGGAGACACACTGAGCTGGGCCTTGCCGGTGCT
GTTGCGTCAGGGCGAACTGGCCGAAAGTCGCCAGGAACGCTTCTGGCACGTGACCCCGGGCGCCAGCGTCGACGATCCGC
GCATCGCCCGCGCACCCAAGCAGCGCGAAGCCCTGACCACCCTGGCCCAGCACCCGCACGGGGTGGCGCATCAACTGCTC
AGCAAGCTGATGCTTAACAAGGACAGCCTCAACCTGTTGCTGGCCAAGGAGCTGGTGTACGTCGAGGTCCGCAGCCATGC
GCCCAGCGCACGCCATGAACACTGGCTGGCCCAGCCTGAATTGCCGCTCAACACCGAACAACGCGCCGCTTACGAAGCGA
TCCGCGCCGGGTTCGACAGCTTTCATGCGTTTCTGCTGGCAGGCGTCACCGGCAGCGGCAAGACTGAAGTCTATCTGCAA
CTGATCCGCGAAACCCTCGAAGCCGGCAAGCAGGCGCTGGTGCTGATCCCGGAAATCAACCTCGGCCCGCAGACCCTGGC
GCGCTTCGAGCAACGCTTCAATGCGCGCATTGCGCTGGTCCACTCGGCGGTCAACGACCGCGAACGGCTGGATGCCTGGC
TGGCAGCTCGCGATGGCGAGGCGGACATCATCATCGGCACGCGTTCGGCGCTGTTCACGCCGATGAAAAACCCCGGCCTG
ATCATCATCGACGAAGAACACGACGGCTCCTATAAACAGCAGGAAGGTCTGCGCTATCACGCCCGCGATCTGGCCTTGGT
GCGGGCGCGTCAGGAAAACATTCCGATTGTCCTTGGCTCGGCCACACCGTCCCTGGAAAGCCTGCACAACGCTTACACCG
GCCGCTACGGTCTGCTGCGCCTGAATGAGCGCGCGGGCGGCGCACAGCAGCCGCGCTTCATGCGCCTGGACGTCAAGAGC
CGACCTCTGGACAGCGGCATCTCCGGCCCCATGCAGCAGGCCATCGGCCAGACGCTCGCGGCAGGCCAGCAGGTCTTGGT
ATTCCTCAACCGTCGCGGCTTTGCGCCAACCTTGCTGTGCCACGACTGCGGCTGGATGTCCGGCTGCCAACGCTGCGATG
CACGCATGACCGTGCACCAGCGCTCGGGCGAGCTGCGCTGCCATCACTGCGGGTATGTCGAGCGCGTACCGCGTCAGTGC
CCGTCGTGCGGCAAGGTGGATTTGCGCCCGGTAGGCGCCGGCACCGAGCGCGCCGAAGAGCGCCTGGCGATTCTGTTTCC
GGACTACCCCGTACTGCGTGTCGACCGCGACAGCACGTCGCGCAAGGACGCCATGAACCAGCTGTTCACGACGATTCAGC
GCGGTCAGCCGTGCATTCTGGTCGGCACGCAAATGCTTGCCAAGGGCCATCACTTCCCCCGGGTCACGCTGGTATCGATT
CTGGACGCCGACGGCGGGCTGTTTTCCGGCGACTTCCGCGCCAGCGAGCGCATGGCGCAATTAATCGTGCAGGTGGCAGG
TCGTGCCGGGCGCGCCGAAGAGCCTGGCAAGGTGATCATCCAGACCCACCTGGCCGACCACCCACTGCTGATCCAGCTGA
CCGAGCAGGGTTATTTCGCCTTTGCCGAACAGGCCTTGAGCGAGCGCCGTTCGGCGGGTCTGCCACCGTTTTCACACCTC
GCACTGCTGCGCGCTGAAGCGCACAAGCCGGGACAGGCCGAGGCCTTTCTCGATCAGGCCTGCAGCGATGCGGAGCAACT
AGTCGCGCAGATGGCGCTGGGCGGCATCGAGCTGCTGGGCCCGGTTCCGGCACCGATGGAACGCCGCGCCGGGCGTTATC
GCGCGCAACTGCTGGTGCAGTCCAGCGCCCGCGCGCCCTTGCATAAATTGCTAGCGACCTGGCTGCTCGCGCTGGAGCAA
ATGCCCAGCGGTCGGCAGGTCAGATGGTCGCTGGATGTGGATCCTGTGGATCTTTATTAA

Domains

Predicted by InterproScan.

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

DEAD/DEAH box helicase (DEAD)

(207-367)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.844

100

0.48

Multiple sequence alignment