Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   SD311_RS01860 Genome accession   NZ_CP166245
Coordinates   433736..435106 (+) Length   456 a.a.
NCBI ID   WP_119603751.1    Uniprot ID   A0A418IQ84
Organism   Staphylococcus sp. KG4-3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 428736..440106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SD311_RS01840 (SD311_001840) - 428886..429347 (+) 462 WP_017723054.1 CtsR family transcriptional regulator -
  SD311_RS01845 (SD311_001845) - 429364..429915 (+) 552 WP_017723053.1 UvrB/UvrC motif-containing protein -
  SD311_RS01850 (SD311_001850) - 429908..430918 (+) 1011 WP_017723052.1 protein arginine kinase -
  SD311_RS01855 (SD311_001855) - 430932..433394 (+) 2463 WP_017723051.1 ATP-dependent Clp protease ATP-binding subunit -
  SD311_RS01860 (SD311_001860) radA 433736..435106 (+) 1371 WP_119603751.1 DNA repair protein RadA Machinery gene
  SD311_RS01865 (SD311_001865) - 435187..436245 (+) 1059 WP_017723049.1 PIN/TRAM domain-containing protein -
  SD311_RS01870 (SD311_001870) gltX 436670..438124 (+) 1455 WP_017723048.1 glutamate--tRNA ligase -
  SD311_RS01875 (SD311_001875) cysE 438507..439118 (+) 612 WP_051035288.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50424.87 Da        Isoelectric Point: 7.2146

>NTDB_id=1033943 SD311_RS01860 WP_119603751.1 433736..435106(+) (radA) [Staphylococcus sp. KG4-3]
MAKKKVIFECMACGYQSPKWMGKCPNCGSWNQMEETVEQKAASPKHGVRSRDNISKVQKLNDIKHETTPRVKTNSEEFNR
VLGGGIVNGSLVLIGGDPGIGKSTLLLQICAALSQSKNVLYITGEESLNQTKLRAERLDEDSSQLNVFAETDLEVIHETV
KQLKPDLLVVDSIQTIFHPEISSAPGSVSQVRESTQSLMNIAKQLNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
EHHAYRILRAVKNRFGSTNEMGIFEMQQTGLKGVKNPSEMFLEERSTNVPGSTIVSTMEGTRPLLIEVQALVTPTTFNNP
RRMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVRLTEPAVDLGIVVATASSFKDLTVDGLDCFIGEVGLTGEV
RRVSRIEQRVQEAEKLGFKRVIIPESNIGGWQFPNDIKVIGVKNVHEALKYALTKQ

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=1033943 SD311_RS01860 WP_119603751.1 433736..435106(+) (radA) [Staphylococcus sp. KG4-3]
TTGGCTAAAAAGAAAGTGATTTTTGAATGTATGGCTTGTGGTTACCAGTCTCCAAAGTGGATGGGGAAATGCCCAAACTG
TGGTAGTTGGAATCAAATGGAAGAAACAGTCGAACAAAAGGCCGCGAGTCCAAAACATGGTGTGCGCAGTCGAGACAATA
TTTCTAAAGTGCAAAAATTAAATGACATTAAACACGAAACGACGCCACGAGTAAAAACAAATTCTGAAGAGTTTAATAGA
GTACTCGGTGGCGGTATAGTAAATGGTTCGCTCGTATTAATAGGCGGTGATCCGGGTATTGGTAAATCAACATTACTTTT
ACAAATTTGTGCAGCATTATCACAATCTAAAAATGTGTTGTATATTACAGGTGAGGAATCGTTAAATCAAACCAAATTAA
GAGCAGAAAGATTAGATGAGGACTCAAGTCAATTAAATGTGTTTGCTGAAACAGATTTAGAAGTTATACATGAAACAGTT
AAGCAACTCAAACCAGATCTACTTGTAGTAGACTCAATCCAAACTATTTTCCATCCAGAAATTAGTTCGGCTCCAGGTTC
TGTGTCGCAAGTTAGAGAAAGTACGCAAAGTTTAATGAATATAGCGAAACAATTGAATATAGCTACATTTATAGTGGGGC
ATGTTACGAAAGAAGGTCAAATAGCTGGTCCGCGTTTACTTGAACATATGGTAGATACAGTCCTTTACTTCGAAGGCGAC
GAGCATCACGCGTATCGCATTTTAAGAGCAGTTAAAAACCGTTTTGGCTCTACAAATGAGATGGGTATCTTTGAGATGCA
GCAAACTGGATTAAAAGGAGTTAAGAACCCGTCGGAAATGTTCCTTGAAGAACGTTCGACTAATGTACCTGGTTCAACTA
TTGTCTCTACAATGGAAGGTACGAGACCGTTACTTATAGAAGTACAAGCGTTAGTGACTCCAACTACATTTAATAATCCA
AGACGTATGGCGACAGGGATCGATCATAATAGATTGAGTTTGCTCATGGCAGTGTTAGAAAAGAAAGAGAATTATTTACT
ACAACAACAGGACGCTTATATTAAAGTGGCTGGGGGCGTACGTTTAACAGAACCAGCTGTAGATTTAGGTATTGTTGTTG
CAACTGCATCAAGTTTTAAAGATTTAACAGTTGATGGTTTAGACTGCTTTATTGGAGAAGTAGGACTCACAGGGGAAGTT
AGACGAGTTTCTCGTATAGAGCAACGTGTACAAGAAGCGGAAAAATTAGGGTTTAAGCGTGTAATTATTCCAGAATCAAA
TATTGGTGGATGGCAATTTCCAAATGATATCAAGGTCATTGGTGTTAAGAATGTCCATGAAGCATTAAAATATGCACTAA
CAAAACAATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-219)

Subunit ChlI of Mg-chelatase (ChlI)

(356-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A418IQ84

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.351

100

0.654
  radA Streptococcus pneumoniae Rx1

60.222

98.684

0.594
  radA Streptococcus pneumoniae D39

60.222

98.684

0.594
  radA Streptococcus pneumoniae R6

60.222

98.684

0.594
  radA Streptococcus pneumoniae TIGR4

60.222

98.684

0.594
  radA Streptococcus mitis NCTC 12261

60.222

98.684

0.594
  radA Streptococcus mitis SK321

60.222

98.684

0.594

Multiple sequence alignment