Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AB8P53_RS10875 Genome accession   NZ_CP166198
Coordinates   2193981..2195357 (+) Length   458 a.a.
NCBI ID   WP_024525645.1    Uniprot ID   A0A1X0XL19
Organism   Levilactobacillus brevis strain BD-LB     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2188981..2200357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8P53_RS10850 (AB8P53_10855) - 2189013..2190245 (+) 1233 WP_187371822.1 serine hydrolase -
  AB8P53_RS10855 (AB8P53_10860) - 2190379..2191713 (-) 1335 WP_085763416.1 aminopeptidase C -
  AB8P53_RS10860 (AB8P53_10865) rpiA 2192133..2192819 (-) 687 WP_011667345.1 ribose-5-phosphate isomerase RpiA -
  AB8P53_RS10865 (AB8P53_10870) - 2192827..2193168 (-) 342 WP_043022184.1 hypothetical protein -
  AB8P53_RS10870 (AB8P53_10875) - 2193347..2193886 (+) 540 WP_085763414.1 dUTP diphosphatase -
  AB8P53_RS10875 (AB8P53_10880) radA 2193981..2195357 (+) 1377 WP_024525645.1 DNA repair protein RadA Machinery gene
  AB8P53_RS10880 (AB8P53_10885) - 2195393..2196577 (+) 1185 WP_021741476.1 PIN/TRAM domain-containing protein -
  AB8P53_RS10885 (AB8P53_10890) gltX 2196841..2198331 (+) 1491 WP_011667350.1 glutamate--tRNA ligase -
  AB8P53_RS10890 (AB8P53_10895) cysS 2198486..2199898 (+) 1413 WP_011667351.1 cysteine--tRNA ligase -
  AB8P53_RS10895 (AB8P53_10900) - 2199898..2200305 (+) 408 WP_011667352.1 Mini-ribonuclease 3 -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49788.09 Da        Isoelectric Point: 7.0881

>NTDB_id=1033674 AB8P53_RS10875 WP_024525645.1 2193981..2195357(+) (radA) [Levilactobacillus brevis strain BD-LB]
MAKPRTTYVCQNCDYSSPRYLGRCPNCGEWNTMVEEVVTPAAAKPQSTRTTASGERSHPQLMSEIKHSTESRTKTQMEEL
NRVLGGGIVPGSLILIGGDPGIGKSTLLLQVSGQLSQTGGKVLYVSGEESASQIKMRADRLVVNSDHLYLYPETDMASIR
ANIEQMKPDYVVIDSVQTMQAPGIESAIGSVSQIRAVTGELMQIAKTNGITIFVVGHVTKGGAIAGPKILEHMVDTVLYF
EGDLHHTYRILRAVKNRFGSTNELGIFEMREGGLYEVANPSEIFLEERLKDATGSAIVVSMEGTRPILVEVQALITPSVF
GNAQRTSSGLDRNRVSLLMAVLEKRANLMLQNQDAFLKAAGGVKLDEPAIDLAIALSIASSYRDTATQPTDCFVGEVGLT
GEIRRVNRIEQRVAEAKKLGFKRIFIPKNNLQGWTPDPGIDVVGVSTLRQTLKLALDV

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=1033674 AB8P53_RS10875 WP_024525645.1 2193981..2195357(+) (radA) [Levilactobacillus brevis strain BD-LB]
GTGGCGAAACCAAGAACAACGTATGTGTGTCAAAATTGTGATTATAGCTCACCCCGTTATTTGGGGCGTTGTCCCAATTG
TGGTGAGTGGAACACGATGGTGGAAGAAGTCGTCACACCGGCTGCCGCCAAGCCACAATCAACCCGGACCACTGCTTCTG
GAGAACGGTCACACCCACAACTCATGAGTGAGATTAAACACTCAACGGAAAGCCGAACGAAGACGCAAATGGAAGAACTG
AACCGAGTGTTGGGTGGGGGAATTGTGCCGGGATCGTTGATCCTAATCGGTGGTGATCCTGGAATTGGAAAATCAACCCT
CTTGCTTCAAGTATCGGGGCAACTCAGCCAAACAGGCGGTAAAGTGTTGTATGTTTCTGGAGAAGAAAGTGCGTCACAGA
TTAAAATGCGGGCGGATCGGTTAGTCGTCAATAGCGACCACTTGTATCTTTATCCAGAAACTGATATGGCCAGTATTCGC
GCGAACATTGAACAGATGAAGCCGGACTACGTGGTCATTGATTCCGTTCAGACCATGCAGGCGCCGGGAATCGAGTCGGC
GATTGGTTCGGTCTCACAAATTCGTGCGGTCACGGGTGAGCTGATGCAGATTGCCAAAACCAATGGCATCACTATTTTTG
TGGTGGGGCATGTGACGAAGGGCGGCGCAATTGCCGGTCCTAAGATTCTGGAACACATGGTGGACACGGTCCTTTACTTT
GAAGGTGACCTCCATCATACGTACCGCATTCTCCGAGCGGTCAAGAACCGATTCGGATCAACGAATGAATTAGGTATCTT
TGAAATGCGTGAAGGCGGCTTGTACGAAGTTGCTAATCCGTCAGAAATTTTCTTGGAGGAACGATTGAAGGACGCAACCG
GTTCGGCCATTGTGGTATCAATGGAAGGTACACGGCCAATTTTGGTAGAAGTTCAGGCGTTAATTACACCATCGGTCTTT
GGTAACGCGCAACGCACATCCAGCGGCCTGGATCGTAATCGGGTATCGTTGTTGATGGCTGTGCTAGAAAAACGGGCCAA
TCTAATGCTCCAAAATCAAGATGCATTCTTAAAGGCAGCAGGTGGCGTTAAGCTGGATGAACCGGCGATTGATTTGGCAA
TTGCACTGTCGATTGCATCGAGTTATCGCGATACGGCCACGCAGCCAACGGATTGTTTTGTCGGTGAAGTTGGCCTGACT
GGTGAAATCCGTCGGGTTAATCGAATTGAACAACGGGTTGCGGAAGCGAAAAAGCTGGGCTTTAAGCGGATCTTCATTCC
CAAGAATAACCTTCAAGGGTGGACGCCCGATCCCGGAATTGATGTGGTCGGCGTGTCAACGTTACGACAGACGTTAAAAC
TGGCCTTGGACGTGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(64-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1X0XL19

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

66.667

98.908

0.659
  radA Streptococcus mitis NCTC 12261

66.446

98.908

0.657
  radA Streptococcus pneumoniae Rx1

66.446

98.908

0.657
  radA Streptococcus pneumoniae D39

66.446

98.908

0.657
  radA Streptococcus pneumoniae R6

66.446

98.908

0.657
  radA Streptococcus pneumoniae TIGR4

66.446

98.908

0.657
  radA Bacillus subtilis subsp. subtilis str. 168

64.035

99.563

0.638

Multiple sequence alignment