Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AB8809_RS20700 Genome accession   NZ_CP166097
Coordinates   4597983..4599365 (+) Length   460 a.a.
NCBI ID   WP_349856488.1    Uniprot ID   -
Organism   Pectobacterium aroidearum strain NCPPB 929     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4592983..4604365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8809_RS20680 (AB8809_20675) prfC 4593266..4594855 (-) 1590 WP_349856487.1 peptide chain release factor 3 -
  AB8809_RS20685 (AB8809_20680) - 4595161..4596078 (+) 918 WP_012773163.1 hypothetical protein -
  AB8809_RS20690 (AB8809_20685) - 4596133..4596831 (-) 699 WP_012773162.1 YtjB family periplasmic protein -
  AB8809_RS20695 (AB8809_20690) serB 4596988..4597965 (+) 978 WP_012773161.1 phosphoserine phosphatase -
  AB8809_RS20700 (AB8809_20695) radA 4597983..4599365 (+) 1383 WP_349856488.1 DNA repair protein RadA Machinery gene
  AB8809_RS20705 (AB8809_20700) - 4599414..4599812 (-) 399 WP_349856489.1 VOC family protein -
  AB8809_RS20710 (AB8809_20705) - 4599931..4600857 (+) 927 WP_349856490.1 LysR substrate-binding domain-containing protein -
  AB8809_RS20715 (AB8809_20710) nadR 4600941..4602194 (+) 1254 WP_012773159.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  AB8809_RS20720 (AB8809_20715) - 4602191..4602832 (-) 642 WP_181845069.1 5-formyltetrahydrofolate cyclo-ligase -
  AB8809_RS20730 (AB8809_20725) zapA 4603130..4603459 (-) 330 WP_012773157.1 cell division protein ZapA -
  AB8809_RS20735 (AB8809_20730) - 4603652..4604239 (+) 588 WP_012773156.1 YecA family protein -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49622.16 Da        Isoelectric Point: 7.4218

>NTDB_id=1033387 AB8809_RS20700 WP_349856488.1 4597983..4599365(+) (radA) [Pectobacterium aroidearum strain NCPPB 929]
MAKAVKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLASASVSRSDRLTGYAGESAGISRVQKLSEISLEALPRFSTG
FQEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLSENMKTLYVTGEESLQQVAMRAHRLNLPTQNLNMLSETSIE
QICLIAEQEQPKLMVIDSIQVMHLADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDQ
SMMSNPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALLLSLVSSFRDRPLPQDLVIFGEV
GLAGEIRPVPSGQERITEAAKHGFKRAIVPHANMPKKTPASMQVFGVKKLADALAILDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=1033387 AB8809_RS20700 WP_349856488.1 4597983..4599365(+) (radA) [Pectobacterium aroidearum strain NCPPB 929]
GTGGCAAAAGCCGTCAAACGGGCGTTTGTATGTAATGAATGCGGGGCTGATTACCCGCGCTGGCAAGGGCAGTGCAGCGC
CTGCCATGCCTGGAACACCATTACCGAAGTGCGTCTGGCATCGGCGTCAGTATCACGTTCCGACCGCCTCACCGGCTATG
CGGGTGAGAGTGCTGGCATCAGCCGGGTACAAAAACTTTCGGAAATCAGCCTTGAAGCCCTGCCCCGTTTTTCTACCGGT
TTTCAGGAGTTTGACCGCGTTCTGGGCGGCGGCGTCGTTCCCGGCAGCGCGATTCTGATCGGCGGTAACCCCGGCGCGGG
TAAAAGTACCCTGCTGCTGCAAACGCTCTGCAAGCTGTCAGAGAATATGAAAACCCTGTACGTCACCGGGGAAGAATCCT
TGCAGCAGGTGGCGATGCGGGCACACCGCCTCAATTTACCGACACAGAATCTCAATATGCTGTCGGAAACCAGCATCGAA
CAAATTTGCCTGATTGCCGAGCAGGAACAGCCGAAGCTGATGGTGATCGACTCCATTCAGGTCATGCATCTCGCCGATAT
TCAATCGTCCCCCGGCAGCGTAGCGCAGGTGCGCGAAACCGCAGCCTACCTGACGCGCTTCGCCAAAACGCGCGGCGTCG
CCATCGTCATGGTCGGCCACGTCACCAAAGACGGCTCGCTCGCCGGACCGAAAGTATTAGAACACTGCATCGACTGCTCC
GTGTTGCTGGATGGCGATGCCGACTCCCGATTCCGCACCCTGCGCAGCCATAAAAACCGTTTCGGTGCCGTTAACGAGCT
GGGCGTGTTCGCGATGACGGAGCAAGGACTACGCGAGGTCAGCAATCCGTCGGCGATTTTCCTTAGCCGCGGGGACGAAG
TGACGTCCGGCAGTTCCGTTATGGTGGTGTGGGAAGGCACGCGCCCGCTGCTGGTCGAGATTCAGGCGCTGGTGGATCAA
TCGATGATGTCGAACCCACGCCGTGTGGCGGTCGGGCTGGAGCAAAACCGGTTAGCCATACTGCTAGCGGTGCTGCATCG
CCACGGCGGCTTGCAGATGTCAGATCAGGATGTGTTCGTGAATGTCGTCGGCGGCGTCAAAGTCACCGAAACCAGCGCTG
ATCTGGCGCTGCTGTTGTCACTGGTCTCCAGCTTCCGCGACCGCCCGCTACCACAGGATCTCGTCATCTTCGGTGAGGTC
GGTCTGGCGGGCGAAATCCGCCCGGTTCCCAGCGGACAAGAGCGGATTACCGAAGCCGCCAAGCACGGCTTCAAACGCGC
CATCGTTCCTCATGCCAATATGCCGAAGAAAACGCCTGCCAGTATGCAGGTATTCGGCGTGAAAAAGCTGGCCGACGCGC
TGGCGATCTTAGACGATCTCTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.478

100

0.485
  radA Streptococcus mitis SK321

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus mitis NCTC 12261

43.421

99.13

0.43

Multiple sequence alignment