Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AB8O67_RS14130 Genome accession   NZ_CP166053
Coordinates   2679490..2679915 (-) Length   141 a.a.
NCBI ID   WP_099043264.1    Uniprot ID   -
Organism   Bacillus halotolerans strain XYK2-4     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2674490..2684915
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8O67_RS14100 (AB8O67_14105) - 2674730..2674864 (-) 135 WP_010335019.1 hypothetical protein -
  AB8O67_RS14105 (AB8O67_14110) - 2674942..2675673 (-) 732 WP_044154481.1 SGNH/GDSL hydrolase family protein -
  AB8O67_RS14110 (AB8O67_14115) - 2675920..2676672 (-) 753 WP_059335450.1 N-acetylmuramoyl-L-alanine amidase -
  AB8O67_RS14115 (AB8O67_14120) - 2676867..2677493 (+) 627 WP_024122122.1 TVP38/TMEM64 family protein -
  AB8O67_RS14120 (AB8O67_14125) gnd 2677514..2678407 (-) 894 WP_099043262.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  AB8O67_RS14125 (AB8O67_14130) - 2678758..2679456 (+) 699 WP_369888615.1 DUF308 domain-containing protein -
  AB8O67_RS14130 (AB8O67_14135) nucA/comI 2679490..2679915 (-) 426 WP_099043264.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  AB8O67_RS14135 (AB8O67_14140) sigK 2680092..2680820 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  AB8O67_RS14140 (AB8O67_14145) - 2680954..2681186 (-) 233 Protein_2739 recombinase family protein -
  AB8O67_RS14145 (AB8O67_14150) - 2681144..2681322 (-) 179 Protein_2740 hypothetical protein -
  AB8O67_RS14150 (AB8O67_14155) - 2681525..2682760 (-) 1236 WP_199398736.1 cytochrome P450 -
  AB8O67_RS14155 (AB8O67_14160) - 2682896..2683126 (-) 231 WP_233403685.1 N-acetyltransferase -
  AB8O67_RS14160 (AB8O67_14165) - 2683584..2684144 (+) 561 WP_326139759.1 TetR/AcrR family transcriptional regulator C-terminal domain-containing protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15576.77 Da        Isoelectric Point: 5.2308

>NTDB_id=1033212 AB8O67_RS14130 WP_099043264.1 2679490..2679915(-) (nucA/comI) [Bacillus halotolerans strain XYK2-4]
MKRWMAMFLLAAAVFLCLLGKDMTAPQQVKGAALYDKILYFPLSRYPETGDHIKDAIAEGHSDICTIDREGADKRREESL
KGIPTKPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTKVLFIVQ

Nucleotide


Download         Length: 426 bp        

>NTDB_id=1033212 AB8O67_RS14130 WP_099043264.1 2679490..2679915(-) (nucA/comI) [Bacillus halotolerans strain XYK2-4]
ATGAAAAGATGGATGGCAATGTTTCTTCTGGCGGCAGCGGTTTTTCTTTGTTTGTTGGGAAAGGATATGACGGCTCCGCA
ACAGGTCAAGGGGGCTGCTTTATATGACAAAATTTTATACTTTCCGTTGTCTCGTTATCCGGAAACCGGGGATCATATAA
AAGATGCGATTGCCGAAGGACATTCTGATATATGTACAATTGATCGTGAGGGGGCGGACAAAAGGCGGGAGGAGTCATTA
AAAGGAATTCCGACGAAGCCTGGCTACGATCGGGATGAATGGCCGATGGCAGTTTGTGAAGAAGGAGGCGCCGGAGCCGA
TGTCCGATATGTAACGCCTTCTGATAATCGCGGCGCCGGTTCATGGGTAGGAAATCAAATGAGCAGTTATCCGGATGGCA
CCAAAGTGTTGTTTATTGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.29

87.943

0.539

Multiple sequence alignment