Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   AB8O67_RS05860 Genome accession   NZ_CP166053
Coordinates   1128409..1132113 (+) Length   1234 a.a.
NCBI ID   WP_369888509.1    Uniprot ID   -
Organism   Bacillus halotolerans strain XYK2-4     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1123409..1137113
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8O67_RS05845 (AB8O67_05840) - 1123857..1124171 (-) 315 WP_044156484.1 four-helix bundle copper-binding protein -
  AB8O67_RS05850 (AB8O67_05845) - 1124300..1124737 (-) 438 WP_044156482.1 ferritin family protein -
  AB8O67_RS05855 (AB8O67_05850) addB 1124922..1128422 (+) 3501 WP_326138810.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AB8O67_RS05860 (AB8O67_05855) addA 1128409..1132113 (+) 3705 WP_369888509.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AB8O67_RS05865 (AB8O67_05860) sbcD 1132185..1133363 (+) 1179 WP_059352651.1 exonuclease subunit SbcD -
  AB8O67_RS05870 (AB8O67_05865) sbcC 1133360..1136752 (+) 3393 WP_369888510.1 exonuclease subunit SbcC -
  AB8O67_RS05875 (AB8O67_05870) - 1136766..1137068 (+) 303 WP_044156476.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141450.85 Da        Isoelectric Point: 5.1008

>NTDB_id=1033179 AB8O67_RS05860 WP_369888509.1 1128409..1132113(+) (addA) [Bacillus halotolerans strain XYK2-4]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPVDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGDKA
FFELVDRYTTDRHDLDLQYLVKQVCEYSRSHPDPETWLKSFVHLYDVTEKSAIEELPFYPYVKEDIAMVLDGAKEKLQRA
LELTKAPGGPAPRAENFLDDLAQIDDLIQHQDDFNELYQRVPAVSFKRAKAVKGDEFDPVLLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHVKSLADMEPVIETLVQLVISYGKRFEAAKQEKSLIDFSDLEHYCLSILTAVNEKGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPENSETETELLLIDKSEEADAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFDAVEVSVVLSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLSAGDRNDELYQKLQTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPEWRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKDHQKQLAKWQASASQADWLLPEFDRYQAKTYLDFIGPALVRHRDLESLAEGGAAAVSDISGHPARFAIQMIHSYD
LLDDELEERLEEKSERLEAIRRGEPVPGPFAFEEKARERLAWKYSHHEVTQIRTKQSVSEIKRRREYEDEYSGRAPVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTIEETERTVQHLYEKELLTEEQQSAINIEEIVQFFHTEIGE
KLIGATWKNREVPFSLALPAKEIYPDAKEADEPLLVQGIIDCLYETEDGLYLLDYKTDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTRAVEQIAQTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=1033179 AB8O67_RS05860 WP_369888509.1 1128409..1132113(+) (addA) [Bacillus halotolerans strain XYK2-4]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAGTGGAATGCCATCGTCTCAACCGGCCAGGATATCCT
TGTGGCAGCGGCGGCAGGCTCCGGTAAAACCGCTGTTCTTGTCGAAAGAATGATCCGGAAAATCACGGCGGAAGAAAATC
CGGTTGATGTAGACCGGCTTCTCGTTGTCACCTTTACAAACGCCTCAGCAGCAGAGATGAAACACCGGATCGCGGATGCA
CTGGAAAAAGAGCTGGTGAAACGCCCAGGATCGCTGCATATCCGACGCCAGCTGTCGCTATTAAACCGGGCCAGCATTTC
AACGCTTCATTCGTTTTGCCTGCAGGTGCTGAAAAAATATTACTATCTGATTGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAGCTTCTCGGGGACGAGGTGCTTGATGAGCTGTTTGAGGATGAATATGCAAAGGGTGATAAGGCG
TTCTTTGAGCTTGTCGACCGATACACGACTGACCGGCATGACTTAGATCTGCAATATCTCGTGAAACAGGTGTGTGAGTA
TTCCCGTTCCCACCCTGATCCGGAGACATGGCTGAAAAGTTTTGTTCATTTGTATGATGTAACAGAAAAGAGCGCGATCG
AGGAACTGCCGTTTTATCCGTATGTCAAAGAAGATATTGCGATGGTGCTGGACGGAGCAAAGGAAAAGCTCCAGCGTGCC
CTTGAGCTGACAAAAGCGCCGGGCGGACCGGCGCCGCGCGCTGAAAATTTTCTCGATGATCTCGCGCAGATTGATGACCT
GATTCAGCATCAGGATGACTTCAATGAACTTTATCAGCGCGTGCCCGCTGTCTCCTTTAAGCGTGCAAAAGCGGTGAAAG
GGGACGAGTTTGATCCCGTCTTGCTTGATGAAGCGACAGACTTACGAAATGGCGCCAAAAAACTGCTTGAAAAACTCAAA
ACCGATTACTTCACCAGAAGCCCGGAGCAGCATGTAAAAAGTTTGGCTGACATGGAGCCTGTCATTGAAACACTCGTCCA
GCTTGTTATCAGCTACGGGAAACGGTTTGAAGCCGCGAAGCAGGAGAAATCGCTCATCGATTTTTCTGATTTGGAGCATT
ATTGCCTGTCCATCTTGACCGCTGTGAACGAAAAAGGCGAACGTGAGCCGAGTGAGGCGGCAAGATTTTATCAGGAGCAG
TTTCATGAGGTGCTTGTCGATGAATACCAGGATACCAACCTGGTGCAGGAATCCATTCTTCAGCTCGTCACAAGCGGGCC
GGAAGAGACAGGCAATCTGTTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TTCTCTCCAAGTACAAACGGTTTACAGAGAGCGGTGAAGGTACGGGCCGGAAAATCGATTTGAACAAAAACTTTCGAAGC
AGAGCTGATATTTTAGACAGCACAAACTTTTTGTTCAAACAGCTGATGGGCGGCAAAATCGGCGAGGTTGATTATGATGA
ACAGGCTGAGCTGAAACTCGGAGCGGCGTATCCGGAAAACAGCGAAACGGAAACAGAGCTGCTGTTGATTGACAAGTCAG
AGGAGGCGGACGCAGGTGAGGAAGCGGAAGAGCTGGAAACGGTGCAGTTCGAAGCAAAAGCGATCGCCAAGGAAATACGC
AAGCTGATCTCATCGCCATTTAAGGTATATGACGGGAAAACGAAAACACACCGCAATATTCAATACCGCGATATCGTGAT
TTTGCTGCGATCCATGCCGTGGGCGCCGCAAATCATGGAGGAACTGAGAGCTCAGGGCATACCGGTTTACGCCAATTTAA
CATCAGGCTACTTTGATGCGGTGGAAGTGTCTGTGGTGCTTTCTGTCCTGAAGGTGATTGATAACCCGTATCAGGATATT
CCGCTTGCTTCCGTTCTGCGTTCACCGATTGTCGGTGCTGACGAGAACGAGCTGTCTTTGATCCGGCTTGAAAACAAAAA
GGCGCCGTACTATGAAGCAATGAAAGACTATCTGTCAGCCGGCGACCGAAACGATGAGCTCTATCAAAAGCTGCAAACGT
TTTACGGACATTTGCAAAAGTGGCGCGCTTTTTCGAAAAACCATTCGGTGTCCGAGCTGATCTGGGAAGTGTACCGCGAC
ACAAAATATATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCTAACTTGCGTGTCCTTTATGACCGGGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTTCGCTTCTTGAGGTTTATCGAACGCATGCAGGAACGCGGCGATG
ATCTGGGAACGGCGAGAGCGTTAAGCGAACAGGAGGATGTTGTCCGTTTAATGACGATTCACAGCAGCAAAGGGCTCGAA
TTCCCAGTCGTTTTCACAGCGGGACTCGGCCGGAATTTCAACATGATGGATCTCAACAAATCCTACCTGCTGGACAAGGA
GCTTGGGTTTGGCACGAAATTTATCCATCCTGAATGGCGCATCAGCTATCCGACGCTTCCGCTCATCGCGATGAAGAAAA
AAATGCGCAGGGAACTGCTGTCTGAGGAATTGCGTGTCCTCTATGTTGCTTTAACGAGGGCAAAGGAAAAGCTGTTTCTT
GTAGGCTCATGCAAGGATCATCAGAAACAGCTGGCAAAATGGCAGGCATCCGCGTCCCAAGCTGACTGGCTTCTGCCGGA
ATTTGACCGCTATCAGGCGAAAACGTATTTGGATTTCATCGGACCTGCTCTTGTCAGACACAGAGACTTGGAGTCTTTGG
CTGAGGGCGGCGCGGCAGCGGTTTCTGATATATCAGGCCATCCGGCCCGCTTTGCTATTCAAATGATCCATTCATATGAT
CTGCTTGATGATGAGCTGGAAGAGAGACTGGAAGAAAAAAGCGAACGTCTTGAAGCGATCCGCCGTGGTGAACCGGTTCC
CGGCCCGTTTGCATTTGAGGAAAAAGCTCGCGAGCGGCTGGCCTGGAAGTATTCGCATCACGAGGTGACACAAATTCGCA
CGAAGCAGTCCGTTTCTGAGATTAAGAGAAGAAGAGAGTACGAAGACGAATACAGCGGGCGGGCGCCCGTTAAACCGGCT
GATGGAAGCATTCTGTACAGACGCCCGGCTTTCATGATGAAAAAAGGCCTGACAGCAGCAGAAAAAGGAACAGCTATGCA
TACGGTCATGCAGCATATTCCGCTGACACATGTGCCGACGATAGAAGAAACGGAGCGGACAGTGCAGCATCTTTATGAAA
AAGAGCTGCTCACCGAAGAACAGCAATCCGCTATCAATATTGAGGAAATTGTTCAATTTTTCCATACGGAAATTGGCGAA
AAACTGATCGGCGCTACATGGAAGAACCGCGAAGTTCCATTCAGTTTGGCGCTTCCGGCAAAAGAGATTTATCCTGATGC
GAAAGAGGCGGATGAGCCGCTATTGGTGCAGGGGATCATCGATTGTCTCTACGAAACAGAGGATGGCTTATATTTGCTGG
ACTATAAAACAGACCGGATTGAGGGTAAATTCCAGCACGGCTTTGAAGGAGCAGCCCCGATCCTGAAAAAGAGATATGAA
ACGCAAATTCAGCTGTACACAAGGGCCGTTGAACAAATTGCTCAAACGAAGGTAAAAGGGTGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTGACTTTATAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

93.112

100

0.931

Multiple sequence alignment