Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | JNUCC21_RS09015 | Genome accession | NZ_CP166019 |
| Coordinates | 1827168..1827602 (+) | Length | 144 a.a. |
| NCBI ID | WP_136396382.1 | Uniprot ID | - |
| Organism | Bacillus sp. JNUCC-21 | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 1828528..1860017 | 1827168..1827602 | flank | 926 |
| IScluster/Tn | 1828528..1829678 | 1827168..1827602 | flank | 926 |
Gene organization within MGE regions
Location: 1827168..1860017
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JNUCC21_RS09015 (JNUCC21_09035) | nucA/comI | 1827168..1827602 (+) | 435 | WP_136396382.1 | NucA/NucB deoxyribonuclease domain-containing protein | Machinery gene |
| JNUCC21_RS09020 (JNUCC21_09040) | - | 1827662..1828417 (+) | 756 | WP_369878752.1 | YoaK family protein | - |
| JNUCC21_RS09025 (JNUCC21_09045) | - | 1828528..1829678 (+) | 1151 | WP_369878753.1 | IS3 family transposase | - |
| JNUCC21_RS09030 (JNUCC21_09050) | - | 1829740..1830102 (-) | 363 | WP_007410383.1 | hypothetical protein | - |
| JNUCC21_RS09035 (JNUCC21_09055) | - | 1830295..1831623 (-) | 1329 | WP_012117603.1 | S8 family peptidase | - |
| JNUCC21_RS09040 (JNUCC21_09060) | - | 1831802..1832035 (+) | 234 | WP_015239897.1 | hypothetical protein | - |
| JNUCC21_RS09045 (JNUCC21_09065) | - | 1832291..1832998 (+) | 708 | WP_012117604.1 | poly-gamma-glutamate hydrolase family protein | - |
| JNUCC21_RS09050 (JNUCC21_09070) | - | 1833058..1833510 (+) | 453 | WP_012117605.1 | OsmC family protein | - |
| JNUCC21_RS09055 (JNUCC21_09075) | - | 1833524..1833877 (-) | 354 | WP_007410379.1 | multidrug efflux SMR transporter | - |
| JNUCC21_RS09060 (JNUCC21_09080) | - | 1833895..1834209 (-) | 315 | WP_007410378.1 | multidrug efflux SMR transporter | - |
| JNUCC21_RS09065 (JNUCC21_09085) | - | 1834347..1834664 (-) | 318 | WP_032866021.1 | hypothetical protein | - |
| JNUCC21_RS09070 (JNUCC21_09090) | - | 1834766..1835170 (+) | 405 | WP_014417831.1 | YmaF family protein | - |
| JNUCC21_RS09075 (JNUCC21_09095) | miaA | 1835269..1836213 (+) | 945 | WP_015239900.1 | tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA | - |
| JNUCC21_RS09080 (JNUCC21_09100) | hfq | 1836253..1836474 (+) | 222 | WP_003154064.1 | RNA chaperone Hfq | - |
| JNUCC21_RS09085 (JNUCC21_09105) | - | 1836572..1836847 (+) | 276 | WP_032871539.1 | YmzC family protein | - |
| JNUCC21_RS09090 (JNUCC21_09110) | - | 1836930..1837145 (+) | 216 | WP_003154062.1 | hypothetical protein | - |
| JNUCC21_RS09095 (JNUCC21_09115) | nrdI | 1837405..1837797 (+) | 393 | WP_003154061.1 | class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI | - |
| JNUCC21_RS09100 (JNUCC21_09120) | nrdE | 1837757..1839859 (+) | 2103 | WP_007611605.1 | class 1b ribonucleoside-diphosphate reductase subunit alpha | - |
| JNUCC21_RS09105 (JNUCC21_09125) | nrdF | 1839877..1840866 (+) | 990 | WP_012117608.1 | class 1b ribonucleoside-diphosphate reductase subunit beta | - |
| JNUCC21_RS09110 (JNUCC21_09130) | - | 1840915..1841535 (+) | 621 | WP_369878754.1 | hypothetical protein | - |
| JNUCC21_RS09115 (JNUCC21_09135) | - | 1841585..1842328 (-) | 744 | WP_369878755.1 | N-acetylmuramoyl-L-alanine amidase | - |
| JNUCC21_RS09120 (JNUCC21_09140) | spoVK | 1842635..1843603 (+) | 969 | WP_015417523.1 | stage V sporulation protein K | - |
| JNUCC21_RS09125 (JNUCC21_09145) | hflX | 1843733..1844995 (+) | 1263 | WP_369878756.1 | GTPase HflX | - |
| JNUCC21_RS09130 (JNUCC21_09150) | - | 1845012..1846277 (+) | 1266 | WP_012117612.1 | aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme | - |
| JNUCC21_RS09135 (JNUCC21_09155) | - | 1846387..1846791 (+) | 405 | WP_007410366.1 | MerR family transcriptional regulator | - |
| JNUCC21_RS09140 (JNUCC21_09160) | glnA | 1846849..1848183 (+) | 1335 | WP_007410365.1 | type I glutamate--ammonia ligase | - |
| JNUCC21_RS09145 (JNUCC21_09165) | - | 1848502..1848768 (+) | 267 | Protein_1748 | ArpU family phage packaging/lysis transcriptional regulator | - |
| JNUCC21_RS09150 (JNUCC21_09170) | - | 1848871..1849500 (+) | 630 | WP_041482194.1 | hypothetical protein | - |
| JNUCC21_RS09155 (JNUCC21_09175) | - | 1850171..1850758 (+) | 588 | WP_144664669.1 | hypothetical protein | - |
| JNUCC21_RS09160 (JNUCC21_09180) | - | 1850979..1851572 (+) | 594 | WP_144664668.1 | hypothetical protein | - |
| JNUCC21_RS09165 (JNUCC21_09185) | - | 1851633..1851968 (+) | 336 | WP_064107798.1 | holin family protein | - |
| JNUCC21_RS09170 (JNUCC21_09190) | - | 1852011..1852876 (+) | 866 | Protein_1753 | N-acetylmuramoyl-L-alanine amidase | - |
| JNUCC21_RS09175 (JNUCC21_09195) | - | 1852938..1854257 (+) | 1320 | WP_369878757.1 | Kelch repeat-containing protein | - |
| JNUCC21_RS09180 (JNUCC21_09200) | - | 1854262..1854375 (+) | 114 | Protein_1755 | peptidoglycan-binding protein | - |
| JNUCC21_RS09185 (JNUCC21_09205) | - | 1854428..1854868 (-) | 441 | WP_048367302.1 | SMI1/KNR4 family protein | - |
| JNUCC21_RS09190 (JNUCC21_09210) | - | 1854883..1856709 (-) | 1827 | WP_369878758.1 | T7SS effector LXG polymorphic toxin | - |
| JNUCC21_RS09195 (JNUCC21_09215) | - | 1856771..1857826 (-) | 1056 | WP_369878759.1 | DUF4917 family protein | - |
| JNUCC21_RS09200 (JNUCC21_09220) | - | 1858923..1860017 (-) | 1095 | WP_369878760.1 | L-dopachrome tautomerase-related protein | - |
Sequence
Protein
Download Length: 144 a.a. Molecular weight: 15544.47 Da Isoelectric Point: 7.2418
>NTDB_id=1033119 JNUCC21_RS09015 WP_136396382.1 1827168..1827602(+) (nucA/comI) [Bacillus sp. JNUCC-21]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISTGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADVRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISTGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADVRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ
Nucleotide
Download Length: 435 bp
>NTDB_id=1033119 JNUCC21_RS09015 WP_136396382.1 1827168..1827602(+) (nucA/comI) [Bacillus sp. JNUCC-21]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGTATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGACAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCAGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATGTCAGATATGTAACCCCATCAGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGTATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGACAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCAGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATGTCAGATATGTAACCCCATCAGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
57.258 |
86.111 |
0.493 |