Detailed information NTG1032609

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: LSAJ54_RS04830
• Genome accession: NZ_LT960790
• Coordinates: 943646..947389 (+)
• Length: 1247 a.a.
• NCBI ID: WP_099947332.1
• Uniprot ID: -
• Organism: Latilactobacillus sakei strain J54
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 938646..952389

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LSAJ54_RS04820 (LSAJ54_0966)	mvk	938874..939821 (-)	948	WP_099947331.1	mevalonate kinase	-
LSAJ54_RS04825 (LSAJ54_0967)	-	940102..943662 (+)	3561	WP_056947677.1	PD-(D/E)XK nuclease family protein	-
LSAJ54_RS04830 (LSAJ54_0968)	addA	943646..947389 (+)	3744	WP_099947332.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
LSAJ54_RS04835 (LSAJ54_0969)	-	947455..950289 (+)	2835	WP_099947333.1	helicase C-terminal domain-containing protein	-
LSAJ54_RS04840 (LSAJ54_0970)	-	950367..950843 (+)	477	WP_011374615.1	DUF5590 domain-containing protein	-
LSAJ54_RS04845 (LSAJ54_0971)	asnS	950954..952252 (+)	1299	WP_011374616.1	asparagine--tRNA ligase	-

Sequence

Protein

• Length: 1247 a.a.
• Molecular weight: 141720.70 Da
• Isoelectric Point: 4.8344

>NTDB_id=1032609 LSAJ54_RS04830 WP_099947332.1 943646..947389(+) (addA) [Latilactobacillus sakei strain J54]
MAILSNPQFTPSQQAAVNHDGHDILVSASAGSGKTSVLVARVIQKILKGTDVDTLLVVTFTEAAATEMRQRIQAALRDAS
EKATEPAVKQRLRQQLSLVPTAQISTLHAFCLKVIKQFYYVIDRDPVFRLLSDTAERLLLADQVWQRVREAFYSHEYVEK
GTQDTLFYELAQNFSNDRNDDGLTDIVFELLDFANANPNPVKWLNELPKSYAVDETGLTASRYFQEKILPILQQTLTECL
EALQEAEQLSQTSENLMVYAPQITTTQAALTQIKADAKTLNWQAWRQQLTDAQLGPAKRTKKLEPDEQINKDRLKADLDD
VKKKIQTLLDTYFVFDEATTTNIQRQAGQLVQKLVRVTLTFREAFQAEKERRHLLDFSDLEQLCLTILSAEDSPARAFYQ
QKFSEVLVDEYQDTNPLQETIIQKVTSDHPRNLFMVGDVKQSIYAFRLADPSLFKNKYNEFGVTEAERDSERIILAENFR
SRRNIDDFTNLIFKQLMDENLGELDYDDNAALQYGARYYPDQHPTAPTELLLYETKPEEAVANPQLDKSEGQVVAVAKRI
QAMMTNGEQIWDKKLEKIRPIEYRDIVLLAPTRGNNLFILDYFKRFGLPVVIKDAQNYFQTTEVQIMLALLQVVDNPNQD
IPLVSVLRSPIVGLNENELALIRINDKTDDYYQAVFNFIDQYNEQKVGHLGQQVMQKLTHFMALLTSFRTIARQQPIVDL
IWTIYQETGFLDYVGGMPAGRQRQANLHALYERATAYEENGFKGLFQFIQFIERLQKQDKDLAQPTSLENQDAISVMTIH
GSKGLEFPVVFLIDTSRRFNQQDLQRSYVLDNHGGLGVVYLDSQKRLKVPTLPELAITAQKRKKLRAEEMRKLYVALTRA
EQWLIIVGHCDNQAQLMKQWQKGQNSTHQILDDGVRNDAASLLDWIGLSLIRHPQAKDWITDYEPLLALSGDQTEFKLIM
TNEALLGEIPGSLHNDQDWGQQQQKALQTIDLNSGIATAIAEQLTFKYPANVATQTTAYQSVSEVKRLFEDPDNTGLGQL
DLAAEQPRQANRYVTDSLAQPQFLQTVQQPTAAEVGTATHLVLQEIDLTQPVTLASLQGLVDRLVLQNSLSANVAAKINL
NHLLTFFESDLGQQLLAHPEAVKREVPFSLLLPAETIFEGIRPTDDDHVLIHGIMDGYLLTDDGCYLFDYKTDFIDPSDQ
EAAIERVKNRYAGQINLYSAALNQIVQKPVTHKYLYLLSIGELVEIN

Nucleotide

• Length: 3744 bp

>NTDB_id=1032609 LSAJ54_RS04830 WP_099947332.1 943646..947389(+) (addA) [Latilactobacillus sakei strain J54]
ATGGCAATCTTGAGTAACCCGCAATTTACACCTTCACAGCAAGCGGCTGTCAATCATGACGGCCACGATATTTTAGTATC
CGCATCAGCTGGTTCAGGGAAGACATCCGTCCTGGTTGCCCGGGTCATCCAGAAAATCTTAAAAGGCACCGATGTCGACA
CCTTGCTTGTTGTGACTTTTACGGAAGCCGCTGCTACTGAAATGCGCCAACGGATTCAAGCCGCACTACGCGATGCCAGT
GAGAAAGCGACCGAGCCAGCTGTTAAACAACGTTTACGGCAACAACTGAGTCTCGTGCCAACCGCACAAATCAGTACGCT
GCATGCTTTTTGTTTAAAAGTCATTAAACAATTTTATTACGTGATTGACCGTGATCCCGTATTCCGCTTATTGAGTGATA
CTGCAGAACGACTCCTCTTAGCGGATCAAGTATGGCAACGGGTGAGAGAAGCATTCTACAGCCACGAGTATGTTGAAAAG
GGCACCCAAGACACCTTATTTTACGAATTGGCCCAAAATTTCTCGAATGATCGTAACGATGACGGCCTAACCGACATTGT
TTTTGAGTTGCTCGATTTTGCAAATGCCAATCCAAACCCGGTTAAATGGTTAAACGAACTACCCAAAAGCTATGCTGTTG
ATGAGACTGGTTTGACTGCCAGTCGCTATTTCCAAGAAAAAATATTACCGATATTACAGCAAACACTCACCGAATGCTTA
GAAGCTTTACAAGAAGCTGAACAGCTCAGTCAAACTAGCGAAAATCTGATGGTTTATGCGCCTCAAATTACCACTACGCA
AGCAGCCTTAACACAAATCAAAGCAGATGCCAAAACGTTAAATTGGCAAGCATGGCGCCAACAACTAACGGATGCCCAGC
TAGGACCTGCTAAACGCACTAAGAAATTAGAGCCTGATGAACAAATTAATAAAGACCGTCTGAAAGCCGATTTAGATGAT
GTCAAAAAGAAAATCCAAACGTTATTAGACACTTATTTTGTGTTTGATGAAGCAACCACCACTAATATTCAGCGCCAAGC
TGGCCAGTTGGTTCAAAAATTGGTGCGCGTCACGTTAACTTTCAGAGAAGCCTTTCAAGCCGAAAAAGAACGACGCCATT
TATTGGACTTTAGTGATTTAGAACAACTCTGTCTGACAATTTTATCGGCAGAAGATTCACCAGCAAGAGCCTTTTATCAA
CAAAAATTTAGCGAAGTATTAGTCGATGAATACCAAGACACGAATCCGCTTCAAGAAACGATTATCCAAAAGGTCACTTC
TGATCATCCGCGCAATCTCTTCATGGTCGGGGATGTTAAGCAGTCAATTTATGCTTTCCGATTGGCCGATCCAAGCTTAT
TTAAGAATAAGTATAACGAATTTGGTGTCACGGAAGCTGAACGTGATAGTGAACGAATCATCTTAGCTGAAAACTTCCGT
TCGCGCCGCAATATCGATGACTTTACCAATCTGATTTTTAAACAATTGATGGACGAAAATCTTGGTGAGTTAGACTACGA
CGACAATGCGGCCTTACAATATGGTGCTCGTTATTATCCTGACCAGCACCCAACCGCGCCAACCGAACTCTTGCTTTATG
AAACTAAGCCGGAAGAGGCAGTTGCCAACCCGCAATTAGATAAGAGTGAGGGCCAAGTCGTGGCGGTCGCTAAACGGATT
CAAGCCATGATGACTAATGGGGAACAGATTTGGGATAAGAAACTCGAAAAAATACGGCCGATTGAATATCGGGACATCGT
TTTACTAGCCCCAACCCGTGGTAATAATTTGTTTATCCTGGACTACTTTAAACGCTTTGGTTTACCGGTTGTGATTAAGG
ATGCGCAAAACTATTTCCAAACGACCGAAGTCCAAATTATGTTAGCTCTACTTCAGGTGGTTGATAATCCTAACCAAGAT
ATTCCACTGGTCAGTGTTTTACGCTCACCAATTGTCGGGTTAAACGAAAATGAATTGGCATTAATTCGGATTAACGATAA
GACGGATGATTATTATCAAGCGGTCTTTAACTTTATTGACCAATATAATGAGCAGAAAGTCGGCCACTTAGGCCAACAAG
TGATGCAGAAGCTGACGCACTTCATGGCTTTATTGACGTCGTTTAGAACAATCGCTCGTCAACAACCGATTGTGGACTTA
ATTTGGACGATCTACCAAGAAACTGGCTTTTTAGATTATGTCGGGGGGATGCCAGCTGGACGTCAACGTCAAGCTAATTT
ACACGCGCTTTATGAACGTGCCACGGCGTATGAAGAAAATGGTTTTAAAGGACTCTTCCAGTTCATTCAGTTTATCGAAC
GTCTTCAAAAACAAGATAAGGATTTAGCACAACCAACGAGCTTAGAAAATCAAGACGCCATCTCTGTTATGACCATTCAC
GGGAGTAAGGGACTTGAATTTCCAGTCGTCTTTTTAATCGATACGAGTCGCCGCTTTAACCAACAGGATTTACAACGCTC
ATATGTTTTGGATAATCATGGTGGTTTAGGGGTCGTTTATTTAGATAGCCAAAAGCGGTTGAAGGTACCAACACTGCCAG
AATTAGCGATTACCGCCCAGAAACGAAAAAAATTACGAGCAGAAGAAATGCGAAAACTATACGTTGCTTTGACACGTGCG
GAACAATGGTTAATCATCGTTGGCCATTGTGATAACCAGGCCCAATTGATGAAGCAATGGCAAAAAGGGCAAAATAGTAC
CCACCAAATATTAGATGATGGTGTTCGCAACGATGCCGCAAGTTTACTGGACTGGATAGGTCTTAGCTTAATTCGCCATC
CACAAGCTAAAGATTGGATCACTGATTATGAGCCACTACTGGCTTTAAGTGGTGATCAAACCGAGTTTAAGTTGATTATG
ACTAATGAAGCCTTACTTGGTGAAATACCAGGTAGCCTGCATAACGATCAGGACTGGGGGCAACAACAGCAAAAGGCACT
TCAAACAATTGATTTAAACAGTGGGATAGCAACAGCGATTGCTGAGCAATTAACCTTTAAGTATCCCGCTAATGTTGCGA
CCCAAACGACGGCTTATCAATCTGTTTCGGAAGTCAAACGCCTTTTTGAAGACCCTGATAATACCGGATTGGGACAATTA
GACCTAGCGGCTGAACAACCGCGCCAAGCAAATCGCTACGTAACGGATAGCTTAGCGCAACCACAGTTCTTACAAACCGT
TCAACAACCAACGGCAGCAGAAGTGGGGACCGCAACGCATTTGGTCTTGCAAGAAATTGATTTAACGCAACCAGTAACGC
TAGCGAGTTTGCAAGGTCTTGTCGATCGACTTGTTTTACAGAATAGTTTAAGCGCCAATGTTGCGGCTAAGATTAATCTT
AATCACCTATTAACCTTCTTTGAAAGTGACTTGGGACAGCAATTATTAGCCCATCCAGAAGCGGTTAAGCGGGAAGTGCC
ATTTTCGTTGTTATTACCAGCAGAAACGATTTTTGAAGGCATTCGTCCCACAGATGACGACCACGTCCTAATCCATGGGA
TTATGGATGGTTATTTATTGACTGACGACGGTTGTTATTTATTTGATTACAAAACCGACTTTATTGACCCCAGTGATCAA
GAAGCGGCGATTGAACGGGTTAAAAACCGCTATGCTGGCCAGATTAACCTCTATAGCGCTGCTTTAAATCAGATTGTCCA
AAAGCCAGTGACCCATAAATATCTTTATTTATTGTCAATTGGCGAACTGGTTGAGATTAACTAG

Domains

Predicted by InterProScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (10-461)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1067-1225)

• UvrD-like helicase C-terminal domain (UvrD_C) (473-887)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	37.363	100	0.382