Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   AB7T04_RS12030 Genome accession   NZ_CP165780
Coordinates   2479280..2479900 (-) Length   206 a.a.
NCBI ID   WP_264101080.1    Uniprot ID   -
Organism   Bacillus velezensis strain L-15     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2474280..2484900
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB7T04_RS12000 (AB7T04_12000) rpsT 2474579..2474845 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  AB7T04_RS12005 (AB7T04_12005) holA 2474862..2475903 (-) 1042 Protein_2316 DNA polymerase III subunit delta -
  AB7T04_RS12010 (AB7T04_12010) - 2475943..2476097 (-) 155 Protein_2317 hypothetical protein -
  AB7T04_RS12015 (AB7T04_12015) - 2476138..2476272 (+) 135 WP_003152870.1 YqzM family protein -
  AB7T04_RS12020 (AB7T04_12020) - 2476291..2478643 (-) 2353 Protein_2319 DNA internalization-related competence protein ComEC/Rec2 -
  AB7T04_RS12025 (AB7T04_12025) - 2478644..2479213 (-) 570 WP_003152868.1 ComE operon protein 2 -
  AB7T04_RS12030 (AB7T04_12030) comEA 2479280..2479900 (-) 621 WP_264101080.1 helix-hairpin-helix domain-containing protein Machinery gene
  AB7T04_RS12035 (AB7T04_12035) comER 2479959..2480780 (+) 822 WP_015240246.1 late competence protein ComER -
  AB7T04_RS12040 (AB7T04_12040) - 2480849..2481586 (-) 738 WP_031378935.1 class I SAM-dependent methyltransferase -
  AB7T04_RS12045 (AB7T04_12045) rsfS 2481583..2481939 (-) 357 WP_003152864.1 ribosome silencing factor -
  AB7T04_RS12050 (AB7T04_12050) yqeK 2481957..2482517 (-) 561 WP_264101081.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  AB7T04_RS12055 (AB7T04_12055) - 2482507..2483076 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  AB7T04_RS12060 (AB7T04_12060) yhbY 2483087..2483377 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  AB7T04_RS12065 (AB7T04_12065) aroE 2483371..2484213 (-) 843 WP_031378933.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 206 a.a.        Molecular weight: 22434.70 Da        Isoelectric Point: 8.4472

>NTDB_id=1031742 AB7T04_RS12030 WP_264101080.1 2479280..2479900(-) (comEA) [Bacillus velezensis strain L-15]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTPSASASVADKKDTADDKIMIDVKGAVRKPG
VYEMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDKLQAISG
VGQKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 621 bp        

>NTDB_id=1031742 AB7T04_RS12030 WP_264101080.1 2479280..2479900(-) (comEA) [Bacillus velezensis strain L-15]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACACCCTCAGCCT
CAGCATCCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAAACCCGGC
GTGTATGAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATT
GAATGTTAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGA
AAACAGCAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACAAACTTCAGGCGATTTCCGGG
GTGGGGCAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAA
TGTATCAGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(143-204)

SLBB domain (SLBB)

(70-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

57.812

93.204

0.539

Multiple sequence alignment