Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AB7T04_RS08695 Genome accession   NZ_CP165780
Coordinates   1765654..1766088 (+) Length   144 a.a.
NCBI ID   WP_007611576.1    Uniprot ID   A0A172XIP3
Organism   Bacillus velezensis strain L-15     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1760654..1771088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB7T04_RS08690 (AB7T04_08690) - 1764154..1765365 (-) 1212 WP_202735983.1 cytochrome P450 -
  AB7T04_RS08695 (AB7T04_08695) nucA/comI 1765654..1766088 (+) 435 WP_007611576.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  AB7T04_RS08700 (AB7T04_08700) - 1766188..1766904 (+) 717 WP_015417517.1 YoaK family protein -
  AB7T04_RS08705 (AB7T04_08705) - 1766938..1767300 (-) 363 WP_007410383.1 hypothetical protein -
  AB7T04_RS08710 (AB7T04_08710) - 1767493..1768821 (-) 1329 WP_071391804.1 S8 family peptidase -
  AB7T04_RS08715 (AB7T04_08715) - 1769000..1769233 (+) 234 WP_071391803.1 hypothetical protein -
  AB7T04_RS08720 (AB7T04_08720) - 1769489..1770196 (+) 708 WP_003154076.1 poly-gamma-glutamate hydrolase family protein -
  AB7T04_RS08725 (AB7T04_08725) - 1770256..1770708 (+) 453 WP_070081858.1 OsmC family protein -
  AB7T04_RS08730 (AB7T04_08730) - 1770722..1771075 (-) 354 WP_003154073.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15528.47 Da        Isoelectric Point: 7.2418

>NTDB_id=1031728 AB7T04_RS08695 WP_007611576.1 1765654..1766088(+) (nucA/comI) [Bacillus velezensis strain L-15]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1031728 AB7T04_RS08695 WP_007611576.1 1765654..1766088(+) (nucA/comI) [Bacillus velezensis strain L-15]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCAGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGCTATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172XIP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486

Multiple sequence alignment