Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AB5985_RS02470 Genome accession   NZ_CP163499
Coordinates   514818..516323 (-) Length   501 a.a.
NCBI ID   WP_027789104.1    Uniprot ID   A0A104AWL9
Organism   Burkholderia cepacia strain JK6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 509818..521323
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB5985_RS02445 (AB5985_02445) - 510844..511479 (-) 636 WP_027789102.1 LON peptidase substrate-binding domain-containing protein -
  AB5985_RS02450 (AB5985_02450) rapZ 511507..512415 (-) 909 WP_021162602.1 RNase adapter RapZ -
  AB5985_RS02455 (AB5985_02455) hprK 512491..513459 (-) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  AB5985_RS02460 (AB5985_02460) - 513591..514130 (-) 540 WP_034195946.1 PTS sugar transporter subunit IIA -
  AB5985_RS02465 (AB5985_02465) raiA 514300..514659 (-) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  AB5985_RS02470 (AB5985_02470) rpoN/rpoN1 514818..516323 (-) 1506 WP_027789104.1 RNA polymerase factor sigma-54 Machinery gene
  AB5985_RS02475 (AB5985_02475) lptB 516495..517271 (-) 777 WP_021162599.1 LPS export ABC transporter ATP-binding protein -
  AB5985_RS02480 (AB5985_02480) lptA 517268..517933 (-) 666 WP_021162598.1 lipopolysaccharide transport periplasmic protein LptA -
  AB5985_RS02485 (AB5985_02485) lptC 517954..518568 (-) 615 WP_027789105.1 LPS export ABC transporter periplasmic protein LptC -
  AB5985_RS02490 (AB5985_02490) - 518572..519108 (-) 537 WP_021162596.1 HAD family hydrolase -
  AB5985_RS02495 (AB5985_02495) kdsD 519108..520091 (-) 984 WP_027789106.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54797.94 Da        Isoelectric Point: 4.8767

>NTDB_id=1030090 AB5985_RS02470 WP_027789104.1 514818..516323(-) (rpoN/rpoN1) [Burkholderia cepacia strain JK6]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAA
TNSSSSSSERAERDEPAGADEYDGYASGDGNDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=1030090 AB5985_RS02470 WP_027789104.1 514818..516323(-) (rpoN/rpoN1) [Burkholderia cepacia strain JK6]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TAGCGAGCCCGCTGCGTGTCGCAGCGGACGGATCATTGATCGCGCAGACGCCTCCCGCCCAGAACACCGAGCCCGCTGCG
ACAAACAGCAGCAGTTCGTCGAGCGAGCGCGCCGAGCGCGACGAACCGGCCGGCGCGGACGAATACGACGGTTACGCGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAATCTCGACGAATTCGGCCGCGCGAGCGGCGCGTCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCACAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAAATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGCGCCCGCTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACACGCACGCTC
GCACTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACACGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTCAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCGATTGTCG
AGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGGTTCGTGGTCGCGCGC
CGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A104AWL9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.047

100

0.689

Multiple sequence alignment