Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | AB5991_RS13325 | Genome accession | NZ_CP163447 |
| Coordinates | 2470239..2470649 (-) | Length | 136 a.a. |
| NCBI ID | WP_009967785.1 | Uniprot ID | A0A6I4D881 |
| Organism | Bacillus subtilis strain SD2 | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 2465606..2500394 | 2470239..2470649 | within | 0 |
Gene organization within MGE regions
Location: 2465606..2500394
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AB5991_RS13300 (AB5991_13300) | yqeF | 2465773..2466504 (-) | 732 | WP_003229964.1 | SGNH/GDSL hydrolase family protein | - |
| AB5991_RS13305 (AB5991_13305) | cwlH | 2466756..2467508 (-) | 753 | WP_014480318.1 | N-acetylmuramoyl-L-alanine amidase CwlH | - |
| AB5991_RS13310 (AB5991_13310) | yqeD | 2467695..2468321 (+) | 627 | WP_014480319.1 | TVP38/TMEM64 family protein | - |
| AB5991_RS13315 (AB5991_13315) | gnd | 2468340..2469233 (-) | 894 | WP_014480320.1 | phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) | - |
| AB5991_RS13320 (AB5991_13320) | yqeB | 2469484..2470206 (+) | 723 | WP_014480321.1 | hypothetical protein | - |
| AB5991_RS13325 (AB5991_13325) | nucA/comI | 2470239..2470649 (-) | 411 | WP_009967785.1 | sporulation-specific Dnase NucB | Machinery gene |
| AB5991_RS13330 (AB5991_13330) | sigK | 2470845..2471573 (+) | 729 | WP_013308023.1 | RNA polymerase sporulation sigma factor SigK | - |
| AB5991_RS13335 (AB5991_13335) | - | 2471573..2471671 (+) | 99 | WP_031600702.1 | hypothetical protein | - |
| AB5991_RS13340 (AB5991_13340) | - | 2471668..2471880 (-) | 213 | Protein_2579 | recombinase family protein | - |
| AB5991_RS13345 (AB5991_13345) | fumC | 2472099..2473487 (-) | 1389 | WP_014480325.1 | class II fumarate hydratase | - |
| AB5991_RS13350 (AB5991_13350) | - | 2473654..2474544 (+) | 891 | WP_014480326.1 | LysR family transcriptional regulator | - |
| AB5991_RS13355 (AB5991_13355) | - | 2475486..2475881 (+) | 396 | WP_014480327.1 | VOC family protein | - |
| AB5991_RS13360 (AB5991_13360) | - | 2476621..2477748 (-) | 1128 | WP_014480328.1 | Rap family tetratricopeptide repeat protein | - |
| AB5991_RS13365 (AB5991_13365) | - | 2477930..2479856 (+) | 1927 | Protein_2584 | ribonuclease YeeF family protein | - |
| AB5991_RS13370 (AB5991_13370) | - | 2479870..2480157 (+) | 288 | WP_014480331.1 | hypothetical protein | - |
| AB5991_RS13375 (AB5991_13375) | - | 2480560..2481000 (+) | 441 | WP_014480332.1 | SMI1/KNR4 family protein | - |
| AB5991_RS13380 (AB5991_13380) | - | 2481099..2481551 (+) | 453 | WP_014480333.1 | SMI1/KNR4 family protein | - |
| AB5991_RS13385 (AB5991_13385) | cdiI | 2481648..2482007 (+) | 360 | WP_014480334.1 | ribonuclease toxin immunity protein CdiI | - |
| AB5991_RS13390 (AB5991_13390) | - | 2482079..2482591 (+) | 513 | WP_014477426.1 | hypothetical protein | - |
| AB5991_RS13395 (AB5991_13395) | - | 2482899..2483102 (-) | 204 | WP_123772462.1 | hypothetical protein | - |
| AB5991_RS13400 (AB5991_13400) | - | 2483191..2483424 (+) | 234 | WP_224588641.1 | hypothetical protein | - |
| AB5991_RS13405 (AB5991_13405) | atxG | 2483692..2484269 (+) | 578 | Protein_2592 | suppressor of fused domain protein | - |
| AB5991_RS13410 (AB5991_13410) | - | 2484379..2484669 (+) | 291 | WP_014480337.1 | contact-dependent growth inhibition system immunity protein | - |
| AB5991_RS13415 (AB5991_13415) | istA | 2485510..2487057 (+) | 1548 | WP_014480339.1 | IS21 family transposase | - |
| AB5991_RS13420 (AB5991_13420) | istB | 2487054..2487812 (+) | 759 | WP_014479891.1 | IS21-like element helper ATPase IstB | - |
| AB5991_RS13425 (AB5991_13425) | - | 2488130..2488227 (-) | 98 | Protein_2596 | N-acetylmuramoyl-L-alanine amidase | - |
| AB5991_RS13430 (AB5991_13430) | - | 2488405..2488491 (+) | 87 | WP_072592549.1 | putative holin-like toxin | - |
| AB5991_RS13435 (AB5991_13435) | - | 2488778..2489214 (-) | 437 | Protein_2598 | phage tail tube protein | - |
| AB5991_RS13440 (AB5991_13440) | terS | 2489212..2489777 (-) | 566 | Protein_2599 | phage terminase small subunit | - |
| AB5991_RS13445 (AB5991_13445) | - | 2489904..2490209 (+) | 306 | WP_123772463.1 | hypothetical protein | - |
| AB5991_RS13450 (AB5991_13450) | - | 2490382..2490447 (-) | 66 | Protein_2601 | hypothetical protein | - |
| AB5991_RS13455 (AB5991_13455) | - | 2490602..2491081 (-) | 480 | WP_014480344.1 | hypothetical protein | - |
| AB5991_RS13460 (AB5991_13460) | - | 2491698..2491964 (+) | 267 | WP_033881358.1 | hypothetical protein | - |
| AB5991_RS13465 (AB5991_13465) | - | 2492103..2492255 (-) | 153 | WP_049832653.1 | XtrA/YqaO family protein | - |
| AB5991_RS13470 (AB5991_13470) | - | 2492338..2492460 (-) | 123 | Protein_2605 | RusA family crossover junction endodeoxyribonuclease | - |
| AB5991_RS13475 (AB5991_13475) | - | 2492423..2492671 (-) | 249 | Protein_2606 | hypothetical protein | - |
| AB5991_RS13480 (AB5991_13480) | - | 2492816..2493046 (-) | 231 | WP_224588644.1 | hypothetical protein | - |
| AB5991_RS13485 (AB5991_13485) | - | 2493355..2493535 (-) | 181 | Protein_2608 | hypothetical protein | - |
| AB5991_RS13490 (AB5991_13490) | bltR | 2493743..2494564 (-) | 822 | WP_014480349.1 | multidrug efflux transcriptional regulator BltR | - |
| AB5991_RS13495 (AB5991_13495) | blt | 2494681..2495883 (+) | 1203 | WP_014480350.1 | multidrug efflux MFS transporter Blt | - |
| AB5991_RS13500 (AB5991_13500) | bltD | 2496065..2496523 (+) | 459 | WP_014480351.1 | spermine/spermidine acetyltransferase | - |
| AB5991_RS13505 (AB5991_13505) | yrkA | 2496682..2497986 (-) | 1305 | WP_014480352.1 | hemolysin family protein | - |
| AB5991_RS13510 (AB5991_13510) | yrzO | 2498276..2498419 (-) | 144 | WP_014477437.1 | YrzO family protein | - |
| AB5991_RS13515 (AB5991_13515) | yrdR | 2498437..2499402 (-) | 966 | WP_014480354.1 | DMT family transporter | - |
| AB5991_RS13520 (AB5991_13520) | czcR | 2499528..2500394 (+) | 867 | WP_014480355.1 | LysR family transcriptional regulator CzcR | - |
Sequence
Protein
Download Length: 136 a.a. Molecular weight: 14967.97 Da Isoelectric Point: 5.1853
>NTDB_id=1029868 AB5991_RS13325 WP_009967785.1 2470239..2470649(-) (nucA/comI) [Bacillus subtilis strain SD2]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
Nucleotide
Download Length: 411 bp
>NTDB_id=1029868 AB5991_RS13325 WP_009967785.1 2470239..2470649(-) (nucA/comI) [Bacillus subtilis strain SD2]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
62.609 |
84.559 |
0.529 |