Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AB5B06_RS15440 Genome accession   NZ_CP163419
Coordinates   2316086..2317561 (-) Length   491 a.a.
NCBI ID   WP_043343436.1    Uniprot ID   A0A643FNT8
Organism   Cupriavidus basilensis strain BL-MT-10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2311086..2322561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB5B06_RS15410 - 2311547..2312224 (-) 678 WP_416047533.1 LON peptidase substrate-binding domain-containing protein -
  AB5B06_RS15415 rapZ 2312291..2313208 (-) 918 WP_416047534.1 RNase adapter RapZ -
  AB5B06_RS15420 - 2313328..2313651 (-) 324 WP_150991818.1 PsiF family protein -
  AB5B06_RS15425 hprK 2313840..2314811 (-) 972 WP_006159437.1 HPr(Ser) kinase/phosphatase -
  AB5B06_RS15430 - 2314847..2315302 (-) 456 WP_416047535.1 PTS sugar transporter subunit IIA -
  AB5B06_RS15435 hpf 2315686..2316036 (-) 351 WP_006159435.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  AB5B06_RS15440 rpoN/rpoN1 2316086..2317561 (-) 1476 WP_043343436.1 RNA polymerase factor sigma-54 Machinery gene
  AB5B06_RS15445 lptB 2317696..2318481 (-) 786 WP_416047536.1 LPS export ABC transporter ATP-binding protein -
  AB5B06_RS15450 lptA 2318506..2319150 (-) 645 WP_416047537.1 lipopolysaccharide transport periplasmic protein LptA -
  AB5B06_RS15455 lptC 2319205..2319831 (-) 627 WP_150991856.1 LPS export ABC transporter periplasmic protein LptC -
  AB5B06_RS15460 - 2319847..2320830 (-) 984 WP_150991810.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 491 a.a.        Molecular weight: 54787.14 Da        Isoelectric Point: 4.9180

>NTDB_id=1029603 AB5B06_RS15440 WP_043343436.1 2316086..2317561(-) (rpoN/rpoN1) [Cupriavidus basilensis strain BL-MT-10]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWLDSPLRVSSDGSVNLQSAPAPAPADGQ
GTSENRPDGAPEADDSYSDSNEDYGSDWSLDDFARRPQGEDDEKTPMQLRDAEPTLREHLMEQLAPLKISLRDKGLAIFL
VESLDDDGYLSTTLEEICSELPEELEFEVDEIQAVLTLLQSFDPPGVGARNAAECLALQLRRLKHPQRELALSIVTNHLE
LLAVRDYTRLKKALQVDEPELKAAHELIRSLAPYPGHAYSRPEADFVVPDVFVRKLSGGWIAHLNPDVMPRLRINDMYAQ
ILRGAKGDAGAAGLQQKLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFTHGEIAMRPLVLREIADTLGLHESTISRVT
TNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDARNPLSDSRIAELLGEQGFVVARRTVAKYREAL
KIPAVNLRKTL

Nucleotide


Download         Length: 1476 bp        

>NTDB_id=1029603 AB5B06_RS15440 WP_043343436.1 2316086..2317561(-) (rpoN/rpoN1) [Cupriavidus basilensis strain BL-MT-10]
ATGAAACCGTCGCTACAGCTCCGCCTCTCGCAGCATCTCGCGCTTACGCCGCAACTCCAGCAGTCGATCCGGCTGCTGCA
ACTGTCCACGCTTGAACTGCAGCAGGAAGTGGAACAGGCGCTGACCGAGAACCCCTTGCTCGAGCGTGAGAACGACTGGC
TGGACAGCCCGTTGCGCGTATCTTCGGACGGCTCGGTGAACCTGCAGAGCGCGCCCGCGCCGGCGCCAGCCGATGGCCAG
GGCACCAGCGAGAACCGCCCCGATGGCGCCCCCGAAGCCGACGACAGCTACTCTGACAGCAATGAGGATTACGGCAGCGA
CTGGAGCCTGGACGACTTCGCACGCCGCCCGCAAGGCGAAGACGACGAAAAAACCCCGATGCAGCTGCGCGATGCCGAGC
CCACGCTGCGTGAACACCTCATGGAGCAGCTGGCGCCGCTCAAGATTTCCCTGCGCGACAAAGGGCTGGCCATCTTCCTG
GTCGAATCCCTGGACGATGACGGCTACCTGAGCACCACGCTCGAGGAAATCTGCAGCGAGCTGCCGGAGGAGCTCGAGTT
CGAGGTCGACGAGATCCAGGCTGTGCTCACGCTGCTGCAGAGCTTCGATCCGCCGGGCGTCGGGGCGCGCAACGCCGCCG
AGTGCCTGGCGCTGCAATTGCGCAGGCTCAAGCATCCGCAGCGCGAACTGGCGCTGAGCATCGTCACCAATCACCTGGAG
CTGCTGGCGGTGCGCGATTACACGCGCCTGAAAAAAGCGCTGCAAGTGGACGAGCCGGAACTCAAGGCAGCACATGAACT
GATCCGCTCGCTCGCGCCCTATCCAGGCCACGCCTACAGCCGGCCAGAAGCGGACTTCGTGGTGCCCGATGTTTTTGTTC
GAAAGTTGAGCGGCGGATGGATCGCCCACCTCAACCCGGATGTGATGCCGCGGCTACGCATCAACGACATGTACGCGCAG
ATTTTGCGCGGTGCAAAAGGCGACGCTGGCGCGGCCGGGCTGCAGCAAAAACTGCAGGAAGCACGCTGGTTGATCAAGAA
TATCCAGCAAAGGTTCGATACGATATTGCGCGTCGCACAAGCCATTGTCGAACGTCAAAAGAACTTCTTCACCCACGGTG
AAATTGCGATGCGCCCCTTGGTTTTGCGGGAGATTGCCGATACACTGGGTTTACACGAGTCCACCATCTCTCGGGTGACT
ACCAATAAATACATGGCAACGCCGATGGGGACTTTCGAGCTGAAGTATTTTTTCGGCTCTCACGTATCCACAGAAACCGG
CGGCGCGGCCTCATCAACGGCAATCCGTGCGTTGATCAAGCAATTGATAGGAGCCGAAGACGCTAGGAACCCCTTGTCCG
ACAGCCGGATTGCCGAATTGCTCGGCGAGCAAGGCTTTGTGGTGGCACGGCGTACCGTTGCCAAATACCGCGAAGCGCTC
AAGATTCCCGCGGTAAATCTCCGTAAGACTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(334-489)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-318)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A643FNT8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

78.758

100

0.8

Multiple sequence alignment