Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AACH65_RS01960 Genome accession   NZ_AP027937
Coordinates   391915..393354 (+) Length   479 a.a.
NCBI ID   WP_005336331.1    Uniprot ID   A0A0T6N7F3
Organism   Aeromonas veronii strain VA60     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 386915..398354
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH65_RS01930 (VAWG004_03710) mlaF 387492..388292 (-) 801 WP_005336343.1 phospholipid ABC transporter ATP-binding protein MlaF -
  AACH65_RS01935 (VAWG004_03720) - 388500..389495 (+) 996 WP_058057518.1 KpsF/GutQ family sugar-phosphate isomerase -
  AACH65_RS01940 (VAWG004_03730) kdsC 389495..390049 (+) 555 WP_088868289.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AACH65_RS01945 (VAWG004_03740) lptC 390046..390606 (+) 561 WP_005336338.1 LPS export ABC transporter periplasmic protein LptC -
  AACH65_RS01950 (VAWG004_03750) lptA 390593..391120 (+) 528 WP_040068817.1 lipopolysaccharide transport periplasmic protein LptA -
  AACH65_RS01955 (VAWG004_03760) lptB 391124..391849 (+) 726 WP_005336333.1 LPS export ABC transporter ATP-binding protein -
  AACH65_RS01960 (VAWG004_03770) rpoN/rpoN1 391915..393354 (+) 1440 WP_005336331.1 RNA polymerase factor sigma-54 Machinery gene
  AACH65_RS01965 (VAWG004_03780) hpf 393375..393662 (+) 288 WP_005336323.1 ribosome hibernation promoting factor -
  AACH65_RS01970 (VAWG004_03790) ptsN 393665..394111 (+) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AACH65_RS01975 (VAWG004_03800) rapZ 394157..395023 (+) 867 WP_216960494.1 RNase adapter RapZ -
  AACH65_RS01980 (VAWG004_03810) npr 395044..395316 (+) 273 WP_005336318.1 PTS phosphocarrier protein NPr -
  AACH65_RS01985 (VAWG004_03820) - 395334..397238 (-) 1905 WP_338710936.1 PTS sugar transporter subunit IIA -
  AACH65_RS01990 (VAWG004_03830) - 397346..398086 (-) 741 WP_338710938.1 KDGP aldolase family protein -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53701.64 Da        Isoelectric Point: 4.3442

>NTDB_id=102959 AACH65_RS01960 WP_005336331.1 391915..393354(+) (rpoN/rpoN1) [Aeromonas veronii strain VA60]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEAQETSPEAPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVALED
IQAAVTSDDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLDQYDPQTPWLNEAKEVILEHMELLGNRDYRTLARK
TKLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=102959 AACH65_RS01960 WP_005336331.1 391915..393354(+) (rpoN/rpoN1) [Aeromonas veronii strain VA60]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAACCCTCTGCTGGAGCAGGAAGAGAGTGAGG
CGCAAGAGACCAGTCCGGAAGCGCCGGTCGATGCCAGCCAGCTGGATTCCAGCGATGCCATGGCCCAGGAGCAGATGCCG
GACGAGTTACCGGTGGATACCACCTGGGACGAGGTTTACTCTGCCGGCACCGCCCAGAGTGCGGGCCCCATCCACGATGG
GGAAGATAGCGTCTATCAAGGAGAGACCACCGAAAACCTGCAGGATTATCTGCTGTGGCAGATGCGGCTCACACCGTTCA
GCGATGTGGATGCCGCCATCGCGCTCGCCATAATCGATGCCATCGACGAATCCGGTTATCTGACCGTTGCGCTGGAGGAT
ATCCAGGCTGCCGTCACCAGTGATGACGTAGAGGTGGAACTCGATGAGGTCGAGGCGGTACTCAAGCGGGTTCAGCACTT
CGATCCGGTCGGTATTGCTGCCCGTTCTGTGCAGGAGTGTTTGCTGATCCAGCTGGATCAGTATGACCCGCAGACCCCCT
GGCTCAACGAGGCCAAGGAGGTGATCCTCGAGCACATGGAGCTGCTCGGCAACCGGGATTACCGCACCCTCGCCCGCAAG
ACAAAACTCAAAGAGAGCGACCTCAAGGAGGTGCTGGATTTGATCCAGCTGCTGGAGCCGCGTCCCGGCAACGGCATCAT
CCAGAGCGACTCCCAGTACGTGATCCCCGACGTCTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTCGAGCTCAATCCCG
ACTCCGCCCCCCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCACGCAACGCCCGCAGCAGCACCGACACCCAGTTT
ATCCGCTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTGGAAAGTCGCAATGACACCCTGCTGAAAGTGGCCAG
TTGCATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCCATGGTGCTCAATGACATCGCCG
AAGCGGTGGAGATGCATGAGTCGACCATCTCCCGGGTGACGACCCAGAAGTACATGCATACCCCCCGTGGTATCTTCGAA
TTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6N7F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.988

100

0.491

Multiple sequence alignment