Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AB4X16_RS14360 Genome accession   NZ_CP163408
Coordinates   3109642..3111111 (+) Length   489 a.a.
NCBI ID   WP_005281701.1    Uniprot ID   D4F169
Organism   Edwardsiella tarda strain 1594     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3104642..3116111
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4X16_RS14330 (AB4X16_14330) - 3105072..3106061 (+) 990 WP_005281708.1 calcium/sodium antiporter -
  AB4X16_RS14335 (AB4X16_14335) kdsD 3106114..3107100 (+) 987 WP_047060479.1 arabinose-5-phosphate isomerase KdsD -
  AB4X16_RS14340 (AB4X16_14340) kdsC 3107116..3107682 (+) 567 WP_005297337.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AB4X16_RS14345 (AB4X16_14345) lptC 3107679..3108251 (+) 573 WP_005281705.1 LPS export ABC transporter periplasmic protein LptC -
  AB4X16_RS14350 (AB4X16_14350) lptA 3108226..3108783 (+) 558 WP_005281704.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  AB4X16_RS14355 (AB4X16_14355) lptB 3108790..3109515 (+) 726 WP_005281703.1 LPS export ABC transporter ATP-binding protein -
  AB4X16_RS14360 (AB4X16_14360) rpoN/rpoN1 3109642..3111111 (+) 1470 WP_005281701.1 RNA polymerase factor sigma-54 Machinery gene
  AB4X16_RS14365 (AB4X16_14365) hpf 3111133..3111420 (+) 288 WP_024524583.1 ribosome hibernation promoting factor -
  AB4X16_RS14370 (AB4X16_14370) ptsN 3111555..3112001 (+) 447 WP_005297331.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AB4X16_RS14375 (AB4X16_14375) rapZ 3112109..3112960 (+) 852 WP_005281696.1 RNase adapter RapZ -
  AB4X16_RS14380 (AB4X16_14380) npr 3112968..3113240 (+) 273 WP_005281695.1 PTS phosphocarrier protein NPr -
  AB4X16_RS14385 (AB4X16_14385) rnk 3113417..3113827 (+) 411 WP_035597411.1 nucleoside diphosphate kinase regulator -
  AB4X16_RS14390 (AB4X16_14390) - 3113858..3114373 (-) 516 WP_369115127.1 cytochrome b/b6 domain-containing protein -
  AB4X16_RS14395 (AB4X16_14395) cybC 3114379..3114765 (-) 387 WP_005291336.1 cytochrome b562 -
  AB4X16_RS14400 (AB4X16_14400) deoD 3114979..3115704 (-) 726 WP_005281692.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 489 a.a.        Molecular weight: 55075.91 Da        Isoelectric Point: 4.4068

>NTDB_id=1029563 AB4X16_RS14360 WP_005281701.1 3109642..3111111(+) (rpoN/rpoN1) [Edwardsiella tarda strain 1594]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQEIQQALESNPLLEQLDPNEEAGDDGVVRENTQEDVVTRDDADPLD
SREALEQREMPDELPLDASWDEIYTAGTPSGTGTDYHDDELPVFQGETTQTLQDYLMWQVELTPFSDTDLAIATAIVDAV
DDTGYLSASLEEILESLGDDRVLPEEIEAVLKRIQRFDPVGVAARDLRDCLLVQLSQFADDTPYLNEARSIVDRHLDLLG
NHDFRNLMRSTKLKEEVLKAAMQLIQSLDPRPGLSINTGESDYVIPDVLVHKHQERWVVELNQDSVPRLRINQHYAALGT
QARNDSDSQFIRSNLQEAKWLIKSLESRNDTLLKVSRCIVEQQQAFFEHGPEAMKPMVLADIAQQVEMHESTISRVTTQK
FLHSPRGIFELKYFFSSHVSTEDGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLADQGIMVARRTVAKYRESLSIP
PSNQRKQLV

Nucleotide


Download         Length: 1470 bp        

>NTDB_id=1029563 AB4X16_RS14360 WP_005281701.1 3109642..3111111(+) (rpoN/rpoN1) [Edwardsiella tarda strain 1594]
ATGAAGCAAGGCTTGCAACTCAGACTCAGCCAGCAGTTGGCCATGACGCCCCAACTGCAACAGGCCATTCGCCTGTTGCA
GCTCTCGACGCTGGAGTTACAGCAAGAGATCCAGCAGGCGTTGGAAAGCAATCCTCTGCTGGAACAACTGGATCCCAATG
AGGAAGCCGGCGACGACGGCGTCGTTCGCGAGAATACACAGGAGGATGTCGTCACACGCGATGATGCCGATCCACTGGAT
AGCCGCGAGGCGTTGGAGCAACGTGAGATGCCGGACGAGCTGCCGCTGGATGCCAGCTGGGACGAGATCTATACCGCTGG
CACCCCCTCAGGCACGGGAACGGATTATCATGACGACGAGTTACCCGTGTTTCAGGGAGAGACGACCCAAACGCTGCAGG
ACTATCTGATGTGGCAGGTCGAACTGACCCCCTTCAGTGATACCGATCTGGCCATCGCTACCGCCATCGTCGATGCCGTC
GACGATACCGGCTATCTCAGCGCCTCGTTGGAGGAGATCCTCGAGAGTCTAGGGGACGATCGCGTCCTACCGGAAGAGAT
CGAGGCCGTCCTCAAGCGCATCCAACGTTTTGATCCGGTCGGTGTCGCCGCGCGTGATCTGCGCGACTGCCTACTGGTCC
AACTCTCGCAATTCGCCGACGACACGCCCTATCTCAACGAGGCTCGTAGCATCGTCGATCGCCACCTCGATCTGTTGGGT
AATCACGATTTCCGAAATCTGATGCGTTCGACTAAGCTAAAAGAAGAGGTGCTCAAGGCGGCGATGCAACTGATCCAGAG
CCTCGACCCGCGGCCAGGACTCTCCATCAATACCGGCGAGTCTGACTATGTCATCCCCGACGTGCTGGTGCATAAACATC
AGGAACGCTGGGTGGTTGAGCTCAATCAGGACAGCGTCCCGCGCCTGCGTATCAATCAACATTACGCCGCGCTGGGCACT
CAGGCGCGTAACGATAGCGACAGCCAGTTCATTCGTAGCAATCTGCAAGAAGCGAAGTGGCTGATCAAGAGCCTAGAGAG
CCGTAACGATACCCTGCTGAAGGTATCGCGTTGCATCGTAGAGCAGCAGCAAGCGTTCTTCGAGCACGGGCCGGAGGCGA
TGAAGCCGATGGTGCTGGCAGATATCGCTCAGCAAGTCGAAATGCACGAGTCGACGATCTCACGCGTGACTACGCAGAAG
TTTCTGCACAGCCCGCGCGGAATCTTTGAACTGAAATATTTCTTCTCCAGCCATGTCAGCACCGAGGACGGCGGCGAGGC
CTCCTCCACTGCCATCCGGGCGCTGGTGAAGAAACTGATCGCGGCAGAAAATCCGGCGAAGCCGCTGAGTGATAGCAAAC
TTACCACTCTATTGGCAGATCAGGGTATCATGGTCGCCCGGCGTACTGTCGCAAAATACCGAGAGTCTTTGTCCATTCCA
CCGTCCAATCAGCGTAAACAGCTGGTGTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(329-487)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D4F169

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.367

100

0.481

Multiple sequence alignment