Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AB4670_RS16150 Genome accession   NZ_CP163382
Coordinates   3376538..3377338 (-) Length   266 a.a.
NCBI ID   WP_004738086.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain Y03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3371538..3382338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4670_RS16130 (AB4670_16120) - 3372603..3373301 (-) 699 WP_000058788.1 tetratricopeptide repeat protein -
  AB4670_RS16135 (AB4670_16125) hisS 3373330..3374622 (-) 1293 WP_000095254.1 histidine--tRNA ligase -
  AB4670_RS16140 (AB4670_16130) ispG 3374619..3375734 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  AB4670_RS16145 (AB4670_16135) - 3375756..3376547 (-) 792 WP_031959662.1 helix-turn-helix domain-containing protein -
  AB4670_RS16150 (AB4670_16140) pilF 3376538..3377338 (-) 801 WP_004738086.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  AB4670_RS16155 (AB4670_16145) rlmN 3377354..3378586 (-) 1233 WP_031959661.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  AB4670_RS16160 (AB4670_16150) ndk 3378709..3379140 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  AB4670_RS16165 (AB4670_16155) iscX 3379254..3379454 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  AB4670_RS16170 (AB4670_16160) - 3379554..3380105 (-) 552 WP_000669565.1 phosphatase PAP2 family protein -
  AB4670_RS16175 (AB4670_16165) - 3380130..3381428 (-) 1299 WP_272379168.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30094.44 Da        Isoelectric Point: 9.2307

>NTDB_id=1029481 AB4670_RS16150 WP_004738086.1 3376538..3377338(-) (pilF) [Acinetobacter baumannii strain Y03]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1029481 AB4670_RS16150 WP_004738086.1 3376538..3377338(-) (pilF) [Acinetobacter baumannii strain Y03]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAGCAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTACTTTAAGCGTGCAATTTCATCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCCTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

100

100

1
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726

Multiple sequence alignment