Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AACH75_RS01590 Genome accession   NZ_AP027933
Coordinates   330225..331664 (+) Length   479 a.a.
NCBI ID   WP_005336331.1    Uniprot ID   A0A0T6N7F3
Organism   Aeromonas veronii strain VA7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 325225..336664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH75_RS01560 (VAWG002_02970) mlaF 325802..326602 (-) 801 WP_005336343.1 phospholipid ABC transporter ATP-binding protein MlaF -
  AACH75_RS01565 (VAWG002_02980) - 326810..327805 (+) 996 WP_005336342.1 KpsF/GutQ family sugar-phosphate isomerase -
  AACH75_RS01570 (VAWG002_02990) kdsC 327805..328359 (+) 555 WP_101530526.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AACH75_RS01575 (VAWG002_03000) lptC 328356..328916 (+) 561 WP_005336338.1 LPS export ABC transporter periplasmic protein LptC -
  AACH75_RS01580 (VAWG002_03010) lptA 328903..329430 (+) 528 WP_040068817.1 lipopolysaccharide transport periplasmic protein LptA -
  AACH75_RS01585 (VAWG002_03020) lptB 329434..330159 (+) 726 WP_005336333.1 LPS export ABC transporter ATP-binding protein -
  AACH75_RS01590 (VAWG002_03030) rpoN/rpoN1 330225..331664 (+) 1440 WP_005336331.1 RNA polymerase factor sigma-54 Machinery gene
  AACH75_RS01595 (VAWG002_03040) hpf 331685..331972 (+) 288 WP_005336323.1 ribosome hibernation promoting factor -
  AACH75_RS01600 (VAWG002_03050) ptsN 331975..332421 (+) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AACH75_RS01605 (VAWG002_03060) rapZ 332467..333333 (+) 867 WP_005336320.1 RNase adapter RapZ -
  AACH75_RS01610 (VAWG002_03070) npr 333354..333626 (+) 273 WP_005336318.1 PTS phosphocarrier protein NPr -
  AACH75_RS01615 (VAWG002_03080) degS 333816..334940 (-) 1125 WP_005336297.1 outer membrane-stress sensor serine endopeptidase DegS -
  AACH75_RS01620 (VAWG002_03090) - 335050..336411 (-) 1362 WP_005336295.1 Do family serine endopeptidase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53701.64 Da        Isoelectric Point: 4.3442

>NTDB_id=102903 AACH75_RS01590 WP_005336331.1 330225..331664(+) (rpoN/rpoN1) [Aeromonas veronii strain VA7]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEAQETSPEAPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVALED
IQAAVTSDDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLDQYDPQTPWLNEAKEVILEHMELLGNRDYRTLARK
TKLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=102903 AACH75_RS01590 WP_005336331.1 330225..331664(+) (rpoN/rpoN1) [Aeromonas veronii strain VA7]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAACCCTCTGCTGGAGCAGGAAGAGAGCGAGG
CGCAAGAGACCAGTCCGGAAGCGCCGGTCGATGCCAGCCAGCTGGATTCCAGCGATGCCATGGCCCAGGAGCAGATGCCG
GACGAGTTGCCGGTGGATACCACCTGGGACGAGGTTTACTCTGCCGGCACCGCCCAGAGCGCGGGCCCCATCCATGATGG
GGAAGATAGCGTCTATCAGGGTGAAACCACCGAAAACCTGCAGGATTATCTGCTGTGGCAGATGCGGCTCACACCGTTCA
GCGATGTGGATGCCGCCATCGCGCTCGCCATCATCGATGCCATCGACGAATCCGGTTATCTGACCGTTGCGCTGGAGGAT
ATCCAGGCTGCCGTCACCAGTGATGACGTAGAGGTGGAACTCGATGAGGTCGAGGCGGTACTCAAGCGGGTGCAGCACTT
CGATCCGGTCGGTATTGCTGCCCGTTCTGTGCAGGAGTGTTTGCTGATCCAGCTGGATCAGTATGACCCGCAGACCCCCT
GGCTCAACGAGGCCAAGGAGGTGATCCTCGAGCACATGGAGCTGCTCGGCAACCGGGATTACCGCACCCTTGCCCGCAAG
ACAAAACTCAAGGAGAGCGACCTCAAGGAGGTGCTGGATCTGATCCAGCTGCTGGAGCCGCGCCCCGGCAACGGCATCAT
CCAGAGCGACTCCCAGTACGTGATCCCCGACGTCTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTCGAGCTCAATCCCG
ACTCTGCCCCCCGCATTCGGGTCAACGAGACCTATGCCGCCATGGCCCGCAACGCCCGCAGCAGCACCGACACCCAGTTT
ATTCGCTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTGGAAAGTCGCAATGACACCCTGCTGAAAGTGGCCAG
TTGCATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCGATGGTGCTCAATGACATCGCCG
AAGCGGTGGAGATGCATGAGTCGACCATCTCCCGGGTGACGACCCAGAAGTACATGCATACCCCCCGTGGTATCTTCGAA
TTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6N7F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.988

100

0.491

Multiple sequence alignment