Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AB4851_RS18445 Genome accession   NZ_CP163165
Coordinates   703385..704899 (-) Length   504 a.a.
NCBI ID   WP_414450969.1    Uniprot ID   -
Organism   Burkholderia sp. 22PA0099     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 698385..709899
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4851_RS18420 (AB4851_18405) - 699449..700084 (-) 636 WP_414445659.1 LON peptidase substrate-binding domain-containing protein -
  AB4851_RS18425 (AB4851_18410) rapZ 700119..701012 (-) 894 WP_414450968.1 RNase adapter RapZ -
  AB4851_RS18430 (AB4851_18415) hprK 701086..702054 (-) 969 WP_013699410.1 HPr(Ser) kinase/phosphatase -
  AB4851_RS18435 (AB4851_18420) - 702183..702739 (-) 557 Protein_630 PTS sugar transporter subunit IIA -
  AB4851_RS18440 (AB4851_18425) hpf 702866..703231 (-) 366 WP_414443114.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  AB4851_RS18445 (AB4851_18430) rpoN/rpoN1 703385..704899 (-) 1515 WP_414450969.1 RNA polymerase factor sigma-54 Machinery gene
  AB4851_RS18450 (AB4851_18435) lptB 705101..705877 (-) 777 WP_414443112.1 LPS export ABC transporter ATP-binding protein -
  AB4851_RS18455 (AB4851_18440) lptA 705874..706545 (-) 672 WP_414443111.1 lipopolysaccharide transport periplasmic protein LptA -
  AB4851_RS18460 (AB4851_18445) lptC 706564..707178 (-) 615 WP_414450970.1 LPS export ABC transporter periplasmic protein LptC -
  AB4851_RS18465 (AB4851_18450) - 707182..707721 (-) 540 WP_414450971.1 KdsC family phosphatase -
  AB4851_RS18470 (AB4851_18455) kdsD 707721..708704 (-) 984 WP_414443108.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 504 a.a.        Molecular weight: 55537.45 Da        Isoelectric Point: 4.9483

>NTDB_id=1028971 AB4851_RS18445 WP_414450969.1 703385..704899(-) (rpoN/rpoN1) [Burkholderia sp. 22PA0099]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAIAQNPLLENDEWLASPLRVGADGTLIPQAPPSSAPEPMHG
NGTSSSSSSDSSSERSDRDERNGDDYDSYGSDGGDSSQWNLEDYGRSSSASDDDDLPPLQVQEATTSLREHLSSQLRVTQ
AGPRDRALVMFLIESLDDDGYLGAALDEVMTDLPEELEVDVDELGAALALLHSFDPAGVGARSASECLRLQLLRLDPSPT
RSLSLDIVSQHLELLAARDFTRLRKHLKANDDELRDAHALIRSLEPFPGAAYGKSEADYVVPDIMVRKSGQNWIAELNPE
VVPKLRINHLYANILRNSRGDPSTGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFTHGEIAMRPLVLREIAD
TLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDQKSPLSDSRIAELLAEQGFV
VARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1515 bp        

>NTDB_id=1028971 AB4851_RS18445 WP_414450969.1 703385..704899(-) (rpoN/rpoN1) [Burkholderia sp. 22PA0099]
ATGAAAGCCAGCCTCCAACTTCGCCTGTCGCAGCACCTCGCGCTTACGCCCCAGCTTCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCATCGCCCAGAACCCGCTGCTCGAGAACGACGAGTGGCTCG
CGAGCCCGCTGCGCGTGGGTGCCGACGGCACGCTGATCCCGCAGGCCCCGCCGTCCTCCGCACCCGAGCCGATGCACGGC
AACGGCACGTCGAGCAGCAGCAGTTCGGATTCATCGAGCGAACGATCGGACCGCGACGAGCGTAACGGCGACGATTACGA
CTCCTATGGATCCGACGGCGGCGATTCGAGCCAGTGGAACCTGGAAGATTACGGCCGCTCGTCGAGCGCCTCCGATGACG
ACGATCTCCCGCCGCTGCAGGTGCAGGAAGCCACGACCTCGCTGCGCGAGCATCTTTCATCGCAGTTGCGCGTCACGCAG
GCCGGCCCGCGCGACCGCGCGCTCGTCATGTTCCTGATCGAATCGCTGGACGACGACGGCTATCTCGGCGCGGCGCTCGA
CGAGGTCATGACCGACCTGCCCGAAGAACTCGAAGTGGATGTGGACGAGCTGGGCGCCGCGCTCGCGCTGCTGCACAGCT
TCGATCCGGCCGGCGTCGGCGCGCGCTCGGCTTCCGAATGCCTGCGCCTGCAACTGCTGCGGCTCGATCCGTCCCCCACG
CGCTCGCTGTCGCTCGACATCGTCTCGCAGCATCTCGAACTGCTGGCCGCGCGCGACTTCACGCGGCTGCGCAAGCACCT
GAAGGCCAACGACGACGAGCTGCGCGACGCGCATGCGCTGATCCGCTCGCTCGAACCGTTCCCGGGCGCCGCCTACGGCA
AGTCCGAAGCCGACTACGTGGTGCCCGACATCATGGTCCGCAAGAGCGGCCAGAACTGGATCGCGGAGCTCAATCCGGAG
GTCGTGCCGAAGCTGCGCATCAATCATCTGTACGCCAACATCCTGCGCAACAGCCGCGGCGATCCGAGCACCGGATCGCT
GAAGCAGCAGCTCCAGGAAGCACGCTGGCTGATCAAGAATATCCAGCAGCGTTTCGAGACGATTCTGCGTGTCGCGCAGG
CCATCGTGGAGCGCCAGAAGAACTTTTTTACGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGAT
ACGCTGGGCCTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGTACTCTTGAATT
TAAGTACTTCTTCGGGTCGCACGTTTCGACAGACACGGGGGGCGCTGCCTCTTCCACGGCAATCCGCGCCCTCATCAAGC
AACTGATAGGAGCCGAAGACCAGAAATCTCCTCTATCGGACAGCCGAATAGCCGAACTGCTGGCGGAACAAGGGTTCGTG
GTCGCGCGTCGTACAGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTCAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(347-502)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(140-331)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.928

100

0.679

Multiple sequence alignment