Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AB4850_RS30495 Genome accession   NZ_CP163163
Coordinates   3487437..3488660 (+) Length   407 a.a.
NCBI ID   WP_414443126.1    Uniprot ID   -
Organism   Burkholderia sp. 22PA0106     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3482437..3493660
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4850_RS30465 (AB4850_30450) - 3483104..3483718 (+) 615 WP_414443120.1 50S ribosomal protein L25/general stress protein Ctc -
  AB4850_RS30470 (AB4850_30455) pth 3483805..3484410 (+) 606 WP_414443121.1 aminoacyl-tRNA hydrolase -
  AB4850_RS30475 (AB4850_30460) hisC 3484495..3485562 (+) 1068 WP_414443122.1 histidinol-phosphate transaminase -
  AB4850_RS30480 (AB4850_30465) - 3485697..3485966 (-) 270 WP_414443123.1 YfhL family 4Fe-4S dicluster ferredoxin -
  AB4850_RS30485 (AB4850_30470) coaD 3486032..3486532 (-) 501 WP_414443124.1 pantetheine-phosphate adenylyltransferase -
  AB4850_RS30490 (AB4850_30475) rsmD 3486644..3487258 (-) 615 WP_414443125.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  AB4850_RS30495 (AB4850_30480) pilA 3487437..3488660 (+) 1224 WP_414443126.1 signal recognition particle-docking protein FtsY Machinery gene
  AB4850_RS30500 (AB4850_30485) maiA 3488723..3489367 (-) 645 WP_414443127.1 maleylacetoacetate isomerase -
  AB4850_RS30505 (AB4850_30490) ybiB 3489543..3490523 (+) 981 WP_414443128.1 DNA-binding protein YbiB -
  AB4850_RS30510 (AB4850_30495) leuA 3491005..3492657 (+) 1653 WP_414443129.1 2-isopropylmalate synthase -
  AB4850_RS30515 (AB4850_30500) - 3492846..3493232 (-) 387 WP_414443130.1 nuclear transport factor 2 family protein -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 42634.82 Da        Isoelectric Point: 4.9435

>NTDB_id=1028965 AB4850_RS30495 WP_414443126.1 3487437..3488660(+) (pilA) [Burkholderia sp. 22PA0106]
MFSFFKRLIGSKADDASQDAPADEQTGADAPAVEAAPDAPRPAESRADATPATPAMPAAPAPVVAQPAAPVALAPQVAPP
AASTPTAPAPRADNTAVEIVPPPAPEPAAKKSWIARLRSGLSKTSSNLTNVFVSTKIDEDLYEELETALLMSDAGVDATE
YLLGALRERVKAQRLTDPQQVKAALRTLLVELLQPLEKSLMLGRAQPLVMMIAGVNGAGKTTSIGKLAKHLQSFDQSVLL
AAGDTFRAAAREQLAIWGERNNVTVVQQESGDPAAVIFDAVGAARARKIDVVMADTAGRLPTQLHLMEELRKVKRVVGKA
QADAPHEVLLVIDANTGQNALAQVKAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFSAEE
FADALLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=1028965 AB4850_RS30495 WP_414443126.1 3487437..3488660(+) (pilA) [Burkholderia sp. 22PA0106]
ATGTTCAGCTTCTTCAAACGATTGATCGGCTCGAAGGCCGACGACGCGTCACAAGACGCGCCGGCCGACGAGCAGACCGG
CGCCGACGCCCCGGCCGTCGAGGCCGCGCCCGACGCGCCGCGGCCTGCCGAGTCGCGTGCCGACGCAACACCGGCCACGC
CCGCCATGCCGGCCGCTCCGGCGCCTGTCGTGGCCCAGCCGGCCGCCCCGGTGGCTCTGGCGCCGCAAGTCGCGCCGCCC
GCGGCGAGCACGCCTACGGCCCCCGCACCGCGCGCCGACAACACCGCAGTCGAAATCGTCCCGCCGCCAGCGCCCGAACC
GGCCGCCAAGAAATCGTGGATCGCGCGGCTGCGCTCGGGCCTTTCCAAGACGAGCTCGAACCTGACGAACGTGTTCGTCA
GCACCAAGATCGACGAAGACCTCTACGAGGAACTCGAAACCGCGCTGCTGATGTCCGACGCCGGCGTGGACGCCACCGAA
TACCTGCTCGGCGCGCTGCGCGAGCGCGTCAAGGCACAGCGCCTGACCGATCCGCAGCAGGTCAAGGCGGCGCTGCGCAC
GCTGCTCGTCGAATTGCTCCAGCCGCTCGAGAAATCCCTGATGCTCGGCCGCGCGCAACCGCTCGTGATGATGATCGCGG
GCGTCAACGGCGCGGGCAAGACCACCAGCATCGGCAAGCTGGCCAAGCATCTGCAGAGCTTCGATCAATCGGTGCTGCTG
GCCGCCGGCGACACGTTCCGCGCCGCCGCACGCGAGCAGCTCGCGATCTGGGGCGAGCGCAACAACGTCACGGTGGTGCA
GCAGGAAAGCGGCGATCCGGCCGCGGTGATCTTCGATGCGGTGGGCGCCGCCCGCGCGCGCAAGATCGACGTGGTGATGG
CCGATACGGCCGGCCGCCTGCCGACGCAGTTGCATCTGATGGAAGAACTGCGCAAGGTCAAGCGCGTGGTCGGCAAGGCG
CAGGCCGACGCACCGCACGAGGTGCTGCTCGTGATCGACGCGAACACGGGCCAGAACGCGCTCGCACAGGTCAAGGCCTT
CGACGACGCACTCGGCCTGACGGGCCTGATCGTCACCAAGCTCGACGGCACCGCGAAGGGCGGCATCCTGGCCGCGATCG
CGCGCCAGCGGCCGGTGCCGGTGTACTTCATCGGCGTCGGCGAGAAGGTGGAGGACCTGCAACCGTTCAGCGCCGAGGAA
TTCGCGGACGCGCTGCTCGGCTGA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(126-186)

SRP54-type protein, GTPase domain (SRP54)

(208-407)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

52.927

100

0.555

Multiple sequence alignment